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Comparative endocrinological study on hormone receptors related to reproductive function

Research Project

Project/Area Number 05454021
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 生物形態・構造
Research InstitutionUniversity of Tokyo

Principal Investigator

KAWASHIMA Seiichiro  Univ, of Tokyo, Grad.Sch of Sci.Professor, 大学院・理学系研究科, 教授 (20011554)

Co-Investigator(Kenkyū-buntansha) OKA Yoshitaka  Univ, of Tokyo, Grad.Sch of Sci.Assistant, 大学院・理学系研究科, 助手 (70143360)
PARK Min kyun  Univ, of Tokyo, Grad.Sch of Sci.Assor, Professor, 大学院・理学系研究科, 助教授 (00228694)
MORI Takao  Univ, of Tokyo, Grad.Sch of Sci.Professor, 大学院・理学系研究科, 教授 (80011659)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1994: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsHormone receptor / cDNA cloning / RT-PCR reaction / Hypothalamus / Pituitary / Gonad / Comparative endocrinology / In situ hybridization / 転写制御
Research Abstract

This project aims to clarify the regulatory mechanism of peptide hormone receptors relating reproductive function. The results obtained are as follows.
(1)The whole base sequence of cDNA encoding gonadotropin-releasing hormone (GnRH) was analyzed. The expression of mRNA for GnRH receptors in the pituitary is found to be limited to LH and FSH cells. Furthermore, the expression in the ovarian follicles both maturing and atreticindicates the role of GnRH in follicular maturation and atresia.
(2)Gonadotropin receptor cDNAs were analyzed in various animal species. Sequencing of extracellular domain was completed in the quail, first discovery in avian species. The determination of epitopes of raised antibody for the extracellular domain was partly completed.
Regulation of receptor number by artificial manipulation of photoperiod in wild birds showed the results as expected.
(3)Radioreceptor assay revealed that the uterus contains specific prolactin receptors preferentially in the myometrial cells. The prolactin binding capacity of the uterus was elevated by prolactin and tended to increase by estrogen.
(4)Immunohistochemical study in dwarf gourami revealed that some GnRH neurons showed isotocin immunoreactivity, indicating functional correlation between GnRH and isotocin.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Kawashima,S.et al.: "Photoperiodic regulation of follicle-stimulating hormone binding to......21GC01:General and Comparative Endocrinology" 92. 250-259 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kudo,A.et al.: "Isolation of rat gonadotropin-releasing hormone receptor cDNA having different 5′-noncoding sequence." Zoological Science. 10. 863-867 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Akazome,Y.et al.: "Characterization of cDNA-encoding N-terminal region of the quail........" General and Comparative Endocrinology. 95. 222-231 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawashima,S.and K.Takagi: "Role of sex steroids on the survival,neuritic outgrowth of neurons,and....." Hormones and Behavior. 28. 305-312 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Matsuda,M.et al.: "Enhanced cell proliferation by hyper-prolactinemia in both exocrine and......" European Journal of Endocrinology22GD05:130. 187-194 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Koga,H.et al.: "In situ detection of gonadotropin-releasing hormone(GnRH)receptor mRNA........" Journal of Experimental Zoology. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawashima, S., K.Tsutsui, R.N.Saxna and S.Ishii: "Photoperiodic regulation of follicle-stimulating hormone binding to gonads and of plasma gonadotropin concentration in Indian weaver birds." General and Comparative Endocrinology. 92. 250-259 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kudo, A., M.K.Park, and S.Kawashima: "Isolation of rat gonadotropin-releasing hormone receptor cDNA having different 5'-noncoding sequence." Zoological Science. 10. 863-867 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Akazome, Y., M.K.Park, T.Mori and S.Kawashima: "Characterization of cDNA-encoding N-terminal region of the quail lutropin receptor." General and Comparative Endocrinology. 95. 222-231 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawashima, S.and K.Takagi: "Role of sex steroids on the survival, neuritic outgrowth of neurons, and dopamine neurons in cultured preoptic area and hypothalamus." Hormones and Behavior. 28. 305-312 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Matsuda, M., T.Mori, M.K.Park and S.Kawashima: "Enhanced cell proliferation by hyperprolactinemia in both exocrine and endocrine pancreas in mice." European Journal of Endocrinology. 130. 187-194 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kogo, H., A.Kudo, M.K.Park, T.Mori and S.Kawashima: "In situ detection of gonadotropin-releasing hormone (GnRH) receptor mRNA expression in the rat ovarial follicles." Journal of Experimental Zoology. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Akazome,Y.et al: "Characterization of cDNA-encoding N-terminalregion of the quail lutropin receptor." Grneral and Comparative Endocrinology. 95. 222-231 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawashima,S.et al.: "Role of sex steroids on the survival,neuritic outgrowth of neurons,and dopamine neurons in cultured preoptic area…" Hormones and Behavior. 28. 305-312 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kudo,A,et al.: "In situ hybridigation study of gonadotropin-releasing hormone(GnRH)receptor mRNA in female…" Journal of Reproduction and Development. 40. 149-158 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Maejima,K.,et al.: "Immunohistochemical double-labeling study of gonadotropin-releasing hormone(GnRH)-immunoreactive…" Neuroscience Research. 20. 189-193 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Matsuda,M.,et al.: "Enhanced cell proliferation by hyperprolactinemia in both exocrine and endocrine pancreas in mice." European Journal of Endocrinology. 130. 187-194 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kogo,H.,et al.: "In situ detection of gonadotropin-releasing hormone(GnRH)receptor mRNA expression in the rat…" Journal of Experimental Zoology. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 川島 誠一郎: "老化とホルモン" 東京大学出版会, 152 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 川島 誠一郎: "動物のホルモン" 裳華房, 192 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawashima,S.,et al.: "Photoperiodic regulation of follicle-stimulating hormone binding to gonads and of plasma gonadotropin concentrations in------." General and Comparative Endocrinology. 92. 250-259 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kudo,A.,et al.: "Isolation of rat GnRH receptor cDNA having different 5′-noncoding sequence." Zoological Science. 10. 863-876 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Takagi,K.,et al.: "Culture of rat brain preoptic area neurons:effects of sex steroids." International Journal of Developmental Neurosci.11. 63-70 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Singtripop,T.,et al.: "Prolactin binding sites in normal uterus and the uterus with adenomyosis in mice." Zoological Science. 10. 353-360 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ohta,N.,et al.: "Hormonal modulation of prolyl endopeptidase and dipeptidyl peptidase IV activities in the mouse uterus and ovary." Acta Endocrinologica. 127. 262-266 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shiraishi,K.,et al.: "Developmental changes of testicular gonadotropin receptors and serum gonadotropin levels in two strains of mice." Zoological Science. 10. 497-504 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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