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Experimental analysiss of morphogenesis of hypothalamohypophyseal system

Research Project

Project/Area Number 05454024
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 生物形態・構造
Research InstitutionWaseda University

Principal Investigator

KIKUYAMA Sakae  Sch Educ Waseda Univ.Professor, 教育学部, 教授 (20063638)

Co-Investigator(Kenkyū-buntansha) MATSUDA K  Nippon Med School Research Associate, 助手 (60222303)
TAKAHASHI N  Human Sci.Res Inst Research Associate Waseda Univ., 人間総合科学研究所, 助手 (80267450)
山内 兄人  早稲田大学, 人間科学部, 教授 (10053357)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1994: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsHypothalamus / hypophysis / morphogenesis / Amphibia / neural ridge / neural plate / 下垂体中葉 / 背地反応 / プロラクチン / α-サブユニット / 視床下部-下垂体系 / 下垂体の発生・分化 / 成長ホルモン / POMC細胞 / 糖タンパクホルモン
Research Abstract

Using Bufo embryos of wild type (rich in melanin granules) and of albino (devoid of melanin granules) transplantation experiments were carried out to identify the origin of the pituitary cells. The anterior part of the neural ridge was found to be the almost exclusive source of the secretory cells in the epithelial pituitary. It was also found that the pituitary primordium and the anlage of the hypothalamus are closely apposed at the open neurula stage. Some of anterior neural ridge-originated cell groups were observed to migrate into the hypothalamus long after the formation of neural tube. One of them is known to be LHRH neurons. But there is another cell group which migrates together with the pituitary primordium and penetrates into the anterior hypothalamus when the pituitary primordium attaches to the infundibulum. Although these cells are not characterized, it is highly probable that they differentiate into hypothalamic neurons. Thus, we concluded that the hypothalamus and the hypophysis are in an intimate clonal relationship. In the hypophyseal primordium developed without contact with the hypothalamus, POMC cells did not appear. Without pituitary primordium, neither development of the median eminence nor extension of hypophysiotripic neurons occurred. This indicates that stimulatory signals that induce differentiation of the hypophysis and hypothalamus are bidirectional ; from hypothalamus to the hypophysis and vice versa.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Kikuyama S: "Development of ectopically transplanted primordium of epithelial hypophysis(anteriorneural ridge)in Bufo japonicus embryos" Journal of Experimental Zoology. 266. 216-220 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura K: "Communication between brain and pituitary during early development" Cellular Communication in Reproduction. 5-10 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Arai Y: "Origin and migration of LHRH neurons:experimental evidence" progress in Comparative Endocrinology. 17-20 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kikuyama S: "Prolactin and growth hormone cell function during metamorphosis with special reference to their gene expression" Progress in Comparative Endocrinology. 108-110 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kikuyama S: "Amphibian prolactin and control of its secretion" Perspectives in Comparative Endocrinology. 237-242 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura K: "Induction from posterior hvpothalamus is essential for the development of the pituitary proopiomelanocortin celle of the toad" Cell and Tissue Research. 279. 233-239 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kikuyama S,Inaco H,Jenks B,Kawamura K: "Development of ectopically transplanted primordium of epithelial hypophysis (anterior neural ridge) in Bufo japonicus embryos" J Exp Zool. 266. 216-220 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura K,Kikuyama S: "Communication between brain and pituitary during early development" Cellular Communication in Reproduction. 5-10 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Arai Y,Murakami S,Seki T,Akutsu S,Kikuyama S: "Origin and migration of LHRH neurons : experimental evidence" Progress in Coparative Endocrinology. 17-20 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kikuyama S,Takahashi N.Kato Y,Kobayashi T,Tanaka S,Yamamoto K: "Prolactin and growth hormone cell function during metamorphosis with special reference to their gene expresion" Progress in Comparative Endocrinology. 108-110 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamashita K,Kikuyama S: "Immunohistochemical atudy of ontogeny of pituitary prolactin and growth hormone" Zoological Science. 11. 149-152 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kikuyama S: "Amphibian prolactin and control of its secretion" Perspectives in Comparative Endocrinology. 237-242 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura K,Kikuyama S: "Induction from posterior hypothalamus is essential for the development of the pituitary proopiomelanocortin cells of the toad" Cell Tissue Research. 279. 233-239 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kikuyama,S: "Prolactin and growth hormone cell function during metamorphosis with special reference to their gene expression" Progress in Comparative Endocrinology (Varavudhi and Lorlowhakam eds). 108-110 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Arai,Y: "Origin and migration of LHRH neurons: experimental evidence" Progress in Comparative Endocrinology (Varavudhi and Lorlowhakam eds). 17-20 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamashita,K: "Immunohistochemical study of pituitary prolactin and growth hormone cells in Xenopus laevis" Zoological Science. 11. 149-152 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kikuyama,S: "Amphibian prolactin and control of its secretion" Perspectives in Comparative Endocrinology. 237-242 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawamura,K: "Induction from posterior hypothalamus is essential for the development of the pituitary proopiomelanocortin cells of the toad" Cell and Tissue Research. 279. 233-239 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawamura,K.Kikuyama,S.: "Communication between brain and pituitary during early amphibian development" Cellular communication in Reproduction.Journal of Endocrinology Ltd,Bristol. 5-10 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kikuyama,S.Inaco,H.Jenks,B.Kawamura,K.: "Development of the ectopically transplanted primordium of epithelial hypophysis(anterior neural ridge) in Bufo japonicus embryos" The Journal of Experimental Zoology. 266. 216-220 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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