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Studies of the second principal sigma factor, sigma^<38> (the rpoS gene product) of RNA polymerase in stationary-phase Escherichia coli.

Research Project

Project/Area Number 05454069
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・応用生物化学
Research InstitutionUNIVERSITY OF TOKYO

Principal Investigator

TAKAHASHI Hideo  Institute of Molecular and Cellular Biosciences, Laboratory of Molecular Genetics and Breeding, Professor, 分子細胞生物学研究所, 教授 (90013333)

Co-Investigator(Kenkyū-buntansha) TANAKA Kan  Institute of Molecular and Cellular Biosciences, Laboratory of Molecular Genetic, 分子細胞生物学研究所, 助手 (60222113)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,500,000 (Direct Cost: ¥7,500,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsSigma factor / sigma^<38> / stationary phase / Escherichia coli / promoter / RNA polymerase / functional domain / 主要シグマ因子 / σ38 / プロモーター特異性
Research Abstract

This research was aimed to elucidate the relationships between structure and function of major-type sigma factors, especially a stationary phase-specific sigma^<38> (=rpoS gene product) of Escherichia coli. (1) RpoS protein was verified to be an RNA polymerase sigma factor by in vitro experiments performed using the re-constituted RNA polymerase holoenzyme containing the purified RpoS protein and named as sigma^<38> from its molecular mass (38 kDa). (2) E sigma^<38> recognized many of E sigma^<70>-type promoters whose-10 hexamer appeared to be crucial for the recognition. (3) Chimeric proteins constructed by recombining the rpoD and rpoS genes had a sigma activity and their recognition specificity was that from the expected functional domains of respective sigma protein. (4) Deletion derivatives of sigma^<38> missing a region of several amino acid residues between region 4.2 and the C-terminus showed higher sigma activity than the wild-type sigma^<38>. We proposed a new functional domain in the C-terminal region of major sigma proteins which may function as a modulator of sigma activity. (5) Deletion mutants of sigma^<38> completely missing the C-terminal portion after the region 4.2 still retained sigma activity, being consistent with the fact that sigma^<38> does not require a specific-35 hexamer sequence for its promoter recognition. (6) The rpoD gene products of cyanobacteria were demonstrated to have a sigma activities by in vitro trnscription experiments.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Araya,T.: "Molecular genetic analysis of the rpoD gene from Lactobacillus lactis." Biosci.Biotech.Biochem.57. 88-92 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka,K.: "Heterogeneity of the principal sigma factor in Escherichia coli: the rpoS gene product, σ^<38>,is a second principal sigma factor of RNA polymerase in stationary phase Escherichia coli" Proc.Natl.Acad.Sci.USA.90. 3511-3515 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujita,M.: "Organization and transcription of the principal sigma geme(rpoDA)of Pseudomanas aeruginosa PAO1: Involvement of a σ^<32>-like RNA polymerase in rpoDA gene expression." J.Bacteriol.175. 1069-1074 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakamoto,T.: "Characterization of DNA and multiple rpoD homologs of Microcystis (Synechocystis)strains." Int.J.System.Bacteriol.43. 844-847 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Utsumi,R.: "Stationary phase-specific expression of the fic gene in Escherichia coli K-12 iscontrolled by the rpoS gene product." FEMSMicrobiol.Lett.113. 273-278 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Abe,A.: "Regulation of spvR gene expression of Salmonella virulence plasmid pKDSC50 in Salmonella serovar Cholerasusi." Mol.Microbiol.,. 12. 779-788 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takayanagi,Y.: "Structkure of the 5'upstream region and the regulation of the rpoS gene of Escherichis coli." Mol.Gen.Genet.243. 525-531 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka,K.: "Cloning,analysis,and expression of rpoS gene of Pseudomonas aeruginosa." Gene. 150. 81-85 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Araya-Kojima,T.: "Identification and genetic analysis of Lactococcus lactis rpoD operon" Biosci.Biotech.Biochem.59. 73-77 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujita,M.: "Transcription of the principal sigma factor genes, rpoD and rpoS, in Pseudomons aeruginosa is controlled depending on the growth phase." Mol.Microbiol.13. 1071-1077 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka,K.: "Promoter determinants for Escherichia coli RNA polymerase holoenzyme containing σ^<38>(the rpoS gene product)." Nucleic Acids Res.23. 827-834 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Utsumi,R.: "Mutational analysis of the fic promoter recognized by RpoS (σ^<38>)in iEscherichia coli." Biosci.Biotech.Biochem.59. 1573-1575 (199)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shinkawa,H.: "Purification and characterization of RNA polymerase holoenzy(Eσ^B)from vegetative-phase mycelia of Streptomyces griseius." J.Bichem.118. 488-493 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsinoremas,N.F.: "A sigma factor that modies the circadian expression of a subset of genes in cyanobacteria." EMVO Journal,. (in press.). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ishibashi, N.: "Molecular genetic analysis of the rpoD gene from Lactobacillus lactis." Biosci.Biotech.Biochem.57. 88-92 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka, K.: "Heterogeneity of the principal sigma factor in Escherichia coli : the rpoS gene product, sigma^<38>, is a second principal sigma factor of RNA polymerase in stationary phase Escherichia coli." Proc.Natl.Acad.Sci.USA. 90. 3511-3515 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujita, M.: "Organization and transcription of the principal simga gene (rpoDA) of Pseudomanas aeruginosa PAO1 : Involvement of a sigma^<32>-like RNA polymerase in rpoDA gene expression." J.Bacteriol.175. 1069-1074 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Sakamoto, T.: "Characterization of DNA and multiple rpoD homologs of Microcystis (Synechocystis) strains." Int.J.System.Bacteriol.43. 844-847 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Utsumi, R.: "Stationary phase-specific expression of the fic gene in Escherichia coli K-12 is controlled by the rpoS gene product." FEMSMicrobiol.Lett.113. 273-278 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Abe, A.: "Regulation of spvR gene expression of Salmonella virulence plasmid pKDSC50 in Salmonella serovar Cholerasusi." Mol.Microbiol.12. 779-788 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takayanagi, Y.: "Structure of the 5'upstream region and the regulation of the rpoS gene of Escherichia coli." Mol.Gen.Genet.243. 525-531 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka, K.: "Cloning, analysis, and expression of rpoS gene of Pseudomonas aeruginosa." Gene. 150. 81-85 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Araya-Kojima, T.: "Identification and genetic analysis of Lactococcus lactis rpoD operon." Biosci.Biotech.Biochem.59. 73-77 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Fujita, M.: "Transcription of the principal sigma factor genes, rpoD and rpoS,in Pseudomonas aeruginosa is controlled depending on the growth phase." Mol.Microbiol.13. 1071-1077 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka, K.: "Promoter determinants for Escherichia coli RNA polymerase holoenzyme containing sigma^<38> (the rpoS gene product)." Nucleic Acids Res.23. 827-834 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Utsumi, R.: "Mutational analysis of the fic promoter recognized by RpoS (sigma^<38>) in Escherichia coli." Biosci.Biotech.Biochem.59. 1573-1575 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shinkawa, H.: "Purification and characterization of RNA polymerase holoenzyme (Esigma^B) from vegetative-phase mycelia of Streptomyces griseus." J.Biochem.118. 488-493 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsinoremas, N.F.: "A sigma factor that modifies the circadian expression of a subset of genes in cyanobacteria." EMBO Journal. (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tanaka,K.: "Promoter determinants for Escherichia coli RNA polymerase holoenzyme containing σ38(rpoS gene product)." Nucleic Acids Res.23. 827-834 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Utsumi,R.,: "Mutational analysis of the fic promoter recognized by rpoS(σ38)in Escherichia coli." Biosci.Biotech.Biochem.59. 1573-1575 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shinkawa,H.: "Purification and characterization of RNA polymerase holoenzyme(EσB)from vegetative-phase mycelia of Streptomyces griseus." J.Biochem.118. 488-493 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tsinoremas,N.F.: "A sigma factor that modifies the circadian expression of a subset of genes in cyanobacteria." EMBO,J.(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Abe,A.: "Regulration of spvR gene expression of Salmonella virulence plasmid pKDSC50 in Salmonella serover Chlorelasuis." Mol.Microbiol.,. 12. 779-788 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takayanagi,Y.: "Structure of the 5 upstream region and the regulation of the rpoS gone of Escherichia coli." Mol.Gen.Genet.,. 243. 523-531 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nimura,K.: "Identification of the dnaK gene family in Synechococcus sp.PCC7942" Biochem.Biophys.Res.Commu.201. 466-471 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tanaka,K.: "Cloning,analysis,and expression of rpoS gene of Pseudomonas aeruginosa PA01" Gene. 150. 81-85 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nimura,K.: "Sequence analysis of the third dnaK homolog gene in Synechococcus sp.PCC7942." Biochem.Biophys.Res.Commu.201. 848-854 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tanaka,K.: "Promoter determinants for Escherichia coli RNA polymerase holoenzyme containing σ38(the rpoS gene product)." Nucleic Acids Res.(in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Tanaka,K.: "Heterogeneity of the principal sigma factor in Escherichia coli:the rpoS gene product,σ38,is second principal sigma factor of RNA polymerase in stationaryphase E.coli." Proc.Natl.Acad.Sci.USA. 90. 3511-3515 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Fujita,M.: "Organization and transcription of the principal sigma gene(rpoDA)of Pseudomonas aeruginosa PA01:Involvement of a σ32-like RNA polymerase in rpoDA gene expression." J.Bacteriol.175. 1069-1074 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sakamoto,T.: "Characterization of DNA and multiple rpoD homologs of Microcystis(Synechocystis)strains." Int.J.System.Bacteriol.43. 844-847 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Araya,T.: "Molecular genetic analysis of the rpoD gene from Lactococcus lactis." Biosci.Biotech.Biochem.57. 88-92 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Utsumi,R.: "Stationary phase-specific expression of the fic gene in Escherichia coli K-12 is controlled by the rpoS gene product(σ38)." FEMS Microbiol.Lett.113. 273-278 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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