• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of genes determining sexual dimorphism in estrogen-responsiveness of the hypothalamus

Research Project

Project/Area Number 05454117
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionThe University of Tokyo

Principal Investigator

NISHIHARA Masugi  The University of Tokyo, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (90145673)

Co-Investigator(Kenkyū-buntansha) SHIOTA Kunio  The University of Tokyo, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (80196352)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,100,000 (Direct Cost: ¥6,100,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsHypothalamus / Estrogen / Sex differentiation / Multiunit activity / Luteinizing hormone / Gonadotropin-releasing hormone / cDNA subtraction / Gene expression / DNAサブトラクション
Research Abstract

This study was performed to elucidate brain regions where gonadal steroid estrogen acts by electrophysiological method, and also to elucidate genes of which expression was activated by estrogen by means of subtracted cDNA method. Using mRNAs extracted from the hypothalamus of either neonatal or adult ovariectomized female rats with or without estrogen treatment, cDNA subtraction was carried out. In the hypothalamus of neonatal rats, 52 clones were isolated as estrogen-responsive genes. A part of clones were sequenced and revealed to include a gene of a kind of cellular growth factors. Further, expression of this gene was shown to be rather restricted in the ventromedial nucleus of the hypothalamus. This gene probably plays an important role in sexual differentiaton of the brain at this phase. On the other hand, 32 clones were isolated in adult rats. One of which was found to be a homologue of a gene of a transcription factor, and might be involved in inducing gonadotropin surge and sex behavior. Thus, we could succeeded in identification of two genes involved in estrogen actions on the brain. We are now investigating the function of the products of these genes.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] HirasawaM 他: "The rostral ventrolateral medulla mediates suppression of the circulatory system by the ventromedial nucleus of the hypothalamus" Brain Research. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] NishimuraF 他: "Changes in chemical sensitivity of rat ventromedial hypothalamic neurons by food deprivation" Physiology & Behavior. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] HirasawaM 他: "Neural activity in the VMH associated with suppression of the circulatory system in rats" Physiology & Behavior. (印刷中).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] NishimuraF 他: "Excitability of neurons in the ventromedial nucleus in rat hypothalamic slices:Modulation by amino acids at cerebrospinal fluid levels" Brain Research. 691. 217-222 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] SuzukiM 他: "Hypothalamic gonadotropin-releasing homone gene expression during rat estrous cycle" Endocrine Journal. 42. 789-796 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] YamanouchiK 他: "Molecular cloning of cDNA for inhibin α-subunit from equine ovary" The Journal of Veterinary Medical Science. 57. 905-909 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hirasawa M,et al.: "The rostral ventrolateral medulla mediates suppression of the circulatory system by the ventromedial nucleus of the hypothalamus" Brain Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishimura F,et al.: "Changes in chemical sensitivity of rat ventromedial hypothalamic neurons by food deprivation" Physiology & Behavior. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hirasawa M,et al.: "Neural activity in the VMH associated with suppression of the circulatory system in rats" Physiology & Behavior. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nishimura F.et al.: "Excitability of neurons in the ventromedial nucleus in rat hypothalamic slices : Modulation by amino acids at cerebrospinal fluid levels" Brain Research. 691. 217-222 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzuki M,et al.: "Hypothalamic gonadotropin-releasing hormone gene expression during rat estrous cycle" Endocrine Journal. 42. 789-796 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamanouchi K,et al.: "Molecular cloning of cDNA for inhibin alpha-subunit from equine ovary" The Journal of Veterinary Medical Science. 57. 905-909 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hirasawa M 他: "The rostral ventrolateral medulla mediates suppression of the circulatory system by the ventromedial nucleus of the hypothalamus" Brain Research. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nishimura F 他: "Changes in chemical sensitivity of rat ventromedial hypothalamic neurons by food deprivation" Physiology & Behavior. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hirasawa M 他: "Neural activity in the VMH associated with suppression of the circulatory system in rats" Physiology & Behavior. (印刷中). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nishimura F 他: "Excitability of neurons in the ventromedial nucleus in rat hypothalamic slices:Modulation by amino acids at cerebrospinal fluid levels" Brain Research. 691. 217-222 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suzuki M 他: "Hypothalamic gonadotropin-releasing hormone gene expression during rat estrous cycle" Endocrine Joumal. 42. -789-796 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nishihara M,Sano A,他: "Cessation of the electrical activity of gonadropin-releasing hormone pulse generator during the steroid-induced surge of luteinizing hormone in the rat" Neuroendocrinology. 51. 513-519 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nishihara M,Mori Y,他: "In vivo electrophysiological monitoring of the GnRH pulse generator in rats and goats" Methods in Neurosciences. 20. 114-126 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ikeda A,Matsuyama S,他: "Changes in endogenous growth hormone secretion and onset of puberty in transgenic rats expressing human growth hormone gene" Endocrine Journal. 41. 523-529 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Narita K,Nishihara M,他: "Conominant regulation of running activity and metabolic change by the ventromedial nucleus of the hypothalamus" Brain Research. 642. 230-296 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Mitsushima D,他: "Attenuation of the expression of circadian rhythms by chronic outpus from the VMH in rats" Physiololy and Behavior. 56. 891-899 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.T.O'Byne,M.Nishihara 他: "Ovarian Control of Gonadotropin Hormone-Releasing Hormone Pulse Generator Activity in the Rhesus Monkey:Duration of the Associated Hypothalamic Signal." Neuroendocrinology. 57. 588-592 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Narita,T.Yokawa,M.Nishihara,M.Takahashi: "Interaction between Excitatory and Inhibitory Amino Acids in the Ventromedial Nucleus of the Hypothalamus in Inducing Hyper-Running." Brain Research. 603. 243-247 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] R-Z.Lu,S.Matsuyama,M.Nishihara,M.Takahashi: "Developmental Expression of Activin/Inhibin βA,βB and α Subunits,and Activin receptor-IIB Genes in Preimplantation Mouse Embryos." Biology of Reproduction. 49. 1163-1169 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Nishihara,Y.Mori,M-J Yoo,M.Takahashi: "In vivo Electrophysiological Monitoring of the GnRH Pulse Generator in Rats and Goats." Methods in Neuroscience. 20. 114-126 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Nishihara,A.Sano,F.Kimura: "Cessation of the Electrical Activity of Gonadotropin-Releasing Hormone Pulse Generator during the Steroid-Induced Surge of Luteinizing Hormone in the Rat." Neuroendocrinology. 58(in press). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Narita,M.Nishihara,M.Takahashi: "Concomitant Regulation of Running Activity and Metabolic Change by the Ventromedial Nucleus of the Hypothalamus." Brain Research. (in press). (1994)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi