| Project/Area Number |
05454125
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | OSAKA PREFECTURE UNIVERSITY |
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Principal Investigator |
TAKAMORI Yasuhiko OSAKA PREFECTURE UNIVERSITY,AGRICULTURE,PROFESSOR, 農学部, 教授 (50090460)
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| Co-Investigator(Kenkyū-buntansha) |
MATUYAMA Satoshi OSAKA PREFECTURE UNIVERSITY,AGRICULTURE,RESEARCH ASSOCIATE, 農学部, 助手 (10254442)
OHASHI Fumihoto OSAKA PREFECTURE UNIVERSITY,AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (10126013)
KUBO Kihei OSAKA PREFECTURE UNIVERSITY,AGRICULTURE,ASSOCIATE PROFESSOR, 農学部, 助教授 (40117619)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥4,600,000 (Direct Cost: ¥4,600,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1993: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | companion animals / RT-PCR / fragile sites / oncogenes / tumor markers / feline genomic library / prohibitin / c-kit / 乳腺腫瘍 / 肥満細胞腫 / ホモロジー / クローニング / ネコ乳癌 / c-kit遺伝子 / 癌抑制遺伝子 / 遺伝子マッピング / 染色体脆弱部位 / ネコゲノムDNAライブラリー / RT‐PCR |
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| Research Abstract |
The fragile sites in feline chromosomes were investigated after the treatment of feline fibroblasts with FdU_+ caffeine or aphidicolin. Five novel aphidicolin-sensitive sites were detected at Alp, A2q, C2q, D3q and D4p. Only two FdU-sensitive sites were newly identified at E1p and F1q. The results suggest that there are very few fragile sites in feline chromosomes as compared with human chromosomes. In order to map the feline oncogenes, an EMBL3 genomic library was constructed using feline liver DNA.For the DNA probe, a part of feline prohibitin cDNA was amplified by the RT-PCR methods and cloned into pBluescript KS (+). Using a genomic clone of isolated from the library, the prohibitin gene was mapped at B4q by the FISH method. The nucleotide sequence of the part of feline prohibitin cDNA was compared with those from bovine, equine, rabbit and canine liver. The sequence is highly conserved among the mammals examined. The result suggests that this part of prohibitin is essential for the function of the protein.
The expression of c-kit gene was investigated in various canine tumors as well as normal spleen (positive control) by RT-PCR.The expression was observed in mastocytomas and a myeloma tissue, while no amplification was observed in a mammary tumor, a lymphoma, and a neoplasm in kidney. The results suggest the usefulness of both prohibitin and c-kit probes as the tumor markers.
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