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Biochemical and molcular biological stuides on the mechanisms of induction of mitochondrial biogenesis in higher plants

Research Project

Project/Area Number 05454132
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Applied molecular and cellular biology
Research InstitutionFaculty of Biotechnology, Fukui Prefectural University

Principal Investigator

ASAHI Tadashi  Fukui Prefectural University, Faculty of Biotechnology, Professor, 生物資源学部, 教授 (10023392)

Co-Investigator(Kenkyū-buntansha) IWASAKI Yukimoto  Fukui Prefectural University, Faculty of Biotechnology, Associate Professor, 生物資源学部, 助教授 (20193732)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1995: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1993: ¥4,500,000 (Direct Cost: ¥4,500,000)
Keywordscatalase genes / cytocrome c oxidase / heterotrimeric G protein / F_1ATPase / microbody / mitochondria / seed germination / ヘテロ三重体Gタンパク質 / 遺伝子発現調整 / F_0F_1ATPase / カタラーゼ / シトクロムcオキシダーゼ / 植物遺伝子
Research Abstract

The biogenesis of mitochondria is induced during germination of seeds, such as castor bean seeds, and in cut-wounded blant tissues. In order to elucidate the molecular mechanisms of the induction of biogenesis, we first isolated genes for mitochondrial proteins including some subunits of cytochome c oxidase and F_1 ATPase and analyzed the structure of the genes. Next, we examined how the genes were expressed in germinating seeds and wounded tissues but obtained no valuable data. Therefore, we turned our attention to microbodies, of which the biogenesisi was induced in response to seed germination and wounding of tissues just like mitochondria and investigated the bio-synthesis, induced in response to the stresses, of catalase, a marker enzyme of micro-bodies. We isolated two catalase genes from castor bean. The genes were located in a chromosome at a short distance and had similar structures but were expressed in different organ-specific manners. Both genes were, however, expressed in similar manners during seed germination ; namely, the expression was induced in response to germination. Then, we prepared fusion genes of 5' upstream regions of one of the catalase genes with E.coli beta-glucuronidase (GUS) gene, which were transferred to tobacco plant, and analyzed the regulatory functions of the regions through assay of GUS activity. The results showed the presence of a cis-element for the expression during seed germination in the regions, in addition to the elements for tissue-specific expression and the expression during seed formation. We are now conducting a series of experiments for identification of the cis-element. We also studied heterotrimeric G proteins in the plasma membrane in order to elucidate the signal transduction of tissue-wounding stress to nuclear gene, and obtained clones carrying cDNAs for the alpha and beta subunits from rice. We are now investigating the structure and function of the G protein.

Report

(3 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Tetsuya Kimura: "The presequence of aprecursor to the δ-subunit of sweet potato mitochondrial F_1ATPase is not sufficient for the transport of β-glucuronidase into mitochondria of tobacco, rice and yeast cells" Plant Cell Physiol.34. 345-355 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsuyoshi Nakagawa: "The nuclear gene for subunit Vc of sweet potato cytochrome c xidase" Plant Cell Physiol.34. 621-626 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaharu Suzuki: "Isolation and characterization of two tightly linked catalase genes from castor bean that are differentially regulated" Plant Mol. Biol.25. 507-516 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaharu Suzuki: "Differential regulation of the expression in transgenic tobacco of the gene for β-glucuronidase under the control of the 5′-upstreaem regions of two catalase genes from castor bean" Plant Cell Physiol. 36. 273-279 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Atsushi Ishikawa: "Molecular cloning and characterization of a cDNA for the α subnit of a G protein from rice" Plant Cell Physiol.36. 353-359 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yukimoto Iwasaki: "Molecular cloning and characterization of a cDNA for a rice protein that contains seven repetitive segments of the Trp-Asp forty-amino-acid repeat (WD-40 repeat)" Plant Cell Physiol.36. 505-510 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaharu Suzuki: "Different sets of cis-elements contribute to the expression of a catalase gene from castor bean during seed formation and postembryonic development in transgenic tobacco" Plant Cell Physiol.36. 1067-1074 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Atsushi Ishikawa: "Molecular cloning and characterzation of a cDNA for the β subunit of a G protein from rice" Plant Cell Physiol.37(in press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 鈴木雅晴: "カタラーゼ遺伝子の操作による耐冷・耐病植物の育成" バイオサイエンスとインダストリー. 54. 26-30 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tetsuya Kimura, Shin Takeda, Junko Kyozuka, Tadashi Asahi, Ko Shimamoto and Kenzou Nakamura: "The presequence of a precursor to the delta-subunit of sweet potato mitochondrial F_1ATPase is not sufficient for the transport of beta-glucuronidase (GUS) into mitochondria of sweet potato, rice and yeast cells" Plant Cell Physiol.34. 345-355 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Tsyyoshi Nakagawa, Masayoshi Maeshima, Kenzo Nakagawa and Tadashi Asahi: "The nuclear gene for subunit Vc of sweet potato cytochrome c oxidase" Plant Cell Physiol.34. 621-616 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaharu Suzuki, Takeshi Ario, Tsukaho Hattori, Kenzo Nakamura and Tadashi Asahi: "Isolation and characterization of two tightly linked catalase genes from castor bean that are differentially regulated" Plant Mol.Biol.25. 507-516 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaharu Suzuki, Ryoma Miyamoto, Tsukaho Hattori, Kenzo Nakamura and Tadashi Asahi: "Differential regulation of the expression in trans-genic tobacco of the gene for beta-glucuronidase under the control of the 5'-upstream regions of two catalase genes from castor bean" Plant Cell Physiol.36. 273-279 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Atsushi Ishikawa, Hitoshi Tsubouchi, Yukimoto Iwasaki and Tadashi Asahi: "Molecular cloning and characterization of a cDNA for the alpha subunit of a G protein from rice" Plant Cell Physiol.36. 353-359 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yukimoto Iwasaki, Masayasu Komano, Atsushi Ishikawa, Takuji Sasaki and Tadashi Asahi: "Molecular cloning and characterization of cDNA for a rice protein that contains seven repetitive segments of the Trp-Asp forty-amino-acid repeat (WD repeat)" Plant Cell Physiol.36. 505-510 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaharu Suzuki, yasuhiro Koide, Tsukaho Hattori, Kenzo Nakamura and Tadashi Asahi: "Different sets of cis-elements contribute to the expression of a catalase gene from castor bean during seed formation and postembryonic development in transgenic tobacco" Plant Cell Physiol.36. 1067-1074 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Atsushi Ishikawa, Yukimoto Iwasaki and Tadashi Asahi: "Molecular cloning and characterization of a cDNA for the beta subunit of a G protein from rice" Plant Cell Physiol.37 (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaharu Suzuki, and Tadashi Asahi: "Breeding of chilling and disease-resistant plants by manipulation of catalse gene" Bioscience and Industry. 54 (in Japanese). 26-30 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masaharu Suzuki: "Differential regulation of the expression in transgenic tobacco of the gene for β-glucuronidase under the control of the 5′ -upstreaem regions of two catalase genes from castor bean" Plant Cell Physiol.36. 273-279 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Atsushi Ishikawa: "Molecular cloning and characterization of a cDNA for the α subunit of a G protein from rice" Plant Cell Physiol.36. 353-359 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yukimoto Iwasaki: "Molecular cloning and characterization of cDNA for a rice protein that contains seven repetitive segments of the Trp-Asp forty-amino-acid repeat (WD-40 repeat)" Plant Cell Physiol.36. 505-510 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masaharu Suzuki: "Different sets of cis-elements contribute to the expression of a catalase gene from castor bean during seed formation and postembryonic development in transgenic tobacco" Plant Cell Physiol.36. 1067-1074 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Atsushi Ishikawa: "Molecular cloning and characterization of a cDNA for the β subunit of a G protein from rice" Plant Cell Physiol.37(in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 鈴木雅晴: "カタラーゼ遺伝子の操作による耐冷・耐病植物の育成" バイオサイエンスとインダストリー. 54. 26-30 (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] Tetsuya Kimura: "The presequence of a precursor to the delta-subunit of sweet potato mitochondrial F_1ATPase is not sufficient for the transport of beta-glucuronidase (GUS) into mitochondria of tobacco, rice and yeast cells" Plant Cell Physiol. 34. 345-355 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tsuyoshi Nakagawa: "The nuclear gene for subunit Vcof sweet potato cytochrome c oxidase" Plant Cell Physiol. 34. 621-626 (1993)

    • Related Report
      1994 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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