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Forensic and Serological Study of Identification of Suspects in Sexual Offenses.

Research Project

Project/Area Number 05454237
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Legal medicine
Research InstitutionFUKUI MEDICAL SCHOOL

Principal Investigator

KISHI Koichiro  Fukui Med.Sch.Dept.Legal Med.Prof., 医学部, 教授 (30169841)

Co-Investigator(Kenkyū-buntansha) MADANO Daita  Fukui Med.Sch.Dept.Legal Med.Assistant., 医学部, 助手 (00228074)
YASUDA Toshihiro  Fukui Med.Sch.Dept.Legal Med.Assist.Prof, 医学部, 助教授 (80175645)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1994: ¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1993: ¥4,000,000 (Direct Cost: ¥4,000,000)
KeywordsDNase / Genetic Polymorphism / Genetic Marker / Gene Mapping / Cloning / PCR / Semen / Sexual Offenses / フコシダーゼ / 個人識別 / 遺伝的多型形質 / DNase I型 / 等電点電気泳動法 / DAFO法 / 精液斑 / 混合体液斑
Research Abstract

In order to identify a suspect in sexual offenses, semen and semen-stains are one of the most important materials. At the present time, only two or three genetic markers in semen have been used for individual ization of them, although many more useful markers have been adopted for blood samples for the same purposes.
We found that genetic polymorphisms of deoxyribonuclease I (DNase I), transferrin (TF), alpha-1-antitrypsin (PI), and fucosidase (FUCA1) were the most appropriate for individualization tests from semen samples, because they have several excellent characteristics : nice gene frequencies, resistancy agaist denaturation, a large amount of their contents in semen, and highly sensitive and specific detection methods for their typing. Our research results about DNase I are as follows.
1. We discovered the genetic polymorphism of DNase I.DNase I is controlled by four co-dominat alleles, DNASE1^*1, ^*2, ^*3 and ^*4. The polymorphism was detected in Japanese, other Asiatics, Caucasians and Africans.
2. DNase I polymorphism were expressed in both body fluids (semen, urine, serum and saliva) and organs (pancreas, liver and kidney).
3. We elucidated the structural organization of the gene for DNase I and showed that the gene was ca. 3.2 kb long, it comprised 9 exons separated by 8 introns and its complete sequence was determined.
4. A regional localization of DNase I gene was assigned to 16p13.3 using the PCR.
5. All the four alleles of DNase I was generated by a point mutation (one nucleotide substitution) occured in the coding region. These substitutions resulted in the replacement of amino acid residues of the mature enzyme. Using two methods, mismatched PCR amplification and amplification refractory mutation system (ARMS), we succeeded in discriminating all of the four DNase I alleles.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (63 results)

All Other

All Publications (63 results)

  • [Publications] Yasuda,Toshihiro: "Postmortem concentrations of thiopental in tissues:a sudden death case" International Journal of Legal Medicine. 105. 239-241 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 安田 年博: "エンドヌクレアーゼアイソザイムの高感度活性染色法-Dried agarose film overlay(DAFO)法のDNase,RNaseアイソザイムの生化学的分析への応用" 生化学. 65. 39-42 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 澤崎 一三: "ペルオキシダーゼ標識抗体によるアガロースゲル中の免疫沈降物の増感法:ロケット免疫電気泳動法による尿トランスフェリンの定量" 科学警察研究所報告法科学編. 46. 15-21 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nadano,Daita: "Measurement of deoxyribonuclease I activity in human tissues and body fluids by a single radial enzyme-diffusion method" Clinical Chemistry. 39. 448-452 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nadano,Daita: "Activity measurement and multiplicity detection of human secretory-type ribonuclease based on polycytidylic acid/ethidium bromide fluorescence" Analytical Biochemistry. 212. 111-116 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuda,Toshihiro: "Survey of the association of deoxyribonuclease I polymorphism with disease" Human Heredity. 43. 205-208 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sawazaki,Kazumi: "Deoxyribonuclease I (DNase I) typing from semen stains:low enzyme activity in vaginal fluids does not interfere with seminal DNase I typing from mixture stains" Journal of Forensic Sciences. 38. 1051-1062 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuda,Toshihiro: "Human seminal deoxyribonuclease I (DNase I):purification,enzymological and immunological characterization and origin" Clinica et Chimica Acta. 218. 5-16 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tenjo,Etsuko: "Salivary deoxyribonuclease I polymorphism separated by polyacrylamide gel-isoelectric focusing and detected by the dried agarose film overlay method" Electrophoresis. 14. 1042-1044 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Iida,Reiko: "Kappa marker typing with high-performance Iiquid chromatography:identification of kappa marker specific tryptic peptide from the kappa light chain of immunoglobulin G" Journal of Chromatography. 622. 9-12 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuda,Toshihiro: "Two distinct secretory ribonucleases from human cerebrum:purification,characterization and relationships to other ribonucleases" Biochemical Journal. 296. 617-625 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuda,Toshihiro: "Use of antibodies against synthetic peptides for analysis of molecular multiplicity:human deoxyribonuclease I polymorphism" Electrophoresis. 15. 260-264 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuda,Toshihiro: "Duplication of 1F and 1A2 genes in the vitamin D-binding protein(GC)system" Human Genetics. 93. 361-362 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takeshita,Haruo: "Detection of deoxyribonucleases I and II (DNases I and II) activities in reproductive organs of male rabbits" International Journal of Biochemistry. 26. 1025-1031 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nadano,Daita: "Purification and characterization of human brain ribonuclease inhibitor" Archives of Biochemistry and Biophysics. 312. 421-428 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takeshita,Haruo: "Genetically polymorphic α-L-fucosidase (FUCA1) isozymes detected in blood plasma" Human Genetics. 94. 224-230 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuda,Toshihiro: "Structure of the human deoxyribonuclease I (DNase I) gene:identification of the nucleotide substituion thet generates its classical genetic polymorphism" Annals of Human Genetics. 59. 1-15 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuda,Toshihiro: "Molecular analysis of the third allele of human deoxyribonucleases I polymorphism" Annals of Human Genetics. 59. 139-147 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sawazaki,Kazumi: "Correlation of alpha-1-antitrypsin (PI) polymorphism between blood and semen" Japanese Journal of Legal Medicine. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuda,Toshihiro: "Chromosomal assignment of the human deoxyribonucleases I gene,DNASE1(DNL1),to 16p13.3 using the polymerase chain reaction" Cytogenetics and Cell Genetics. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yaduda,Toshihiro: "The molecular basis for genetic polymorphism of human deoxyribonucleaes I:identification of the nucleotide substitution that generates the fourth allele" FEBS Letters. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yasuda: "Postmortem concentrations of thiopental in tissues : a sudden death case" Int.J.Legal Med.105/4. 239-241 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yasuda: "The highly sensitive zymogram method for detection of endonuclease isozymes : application of dried agarose film overlay (DAFO) method for biochemical analysis of both DNase and RNase isozymes" Seikagaku. 65/1. 39-42 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Sawazaki: "Amplification of immunoprecipitates in agarose gels by peroxidase-labeled antibody : quantitaion of urinary transferrin using roket immunoelectrophoresis" Rep.Natl.Res.Inst.Police Sci.46/1. 15-21 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] D.Nadano: "Measurement of deoxyribonuclease I activity in human tissues and body fluids by a single radial enzyme-diffusion method" Clin.Chem.39/3. 448-452 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] D.Nadano: "Activity measurement and multiplicity detection of human secretory-type ribonuclease based on polycytidylic acid/ethidium bromide fluorescence" Anal.Biochem.212/1. 111-116 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yasuda: "Survey of the association of deoxyribonuclease I polymorphism with disease" Hum.Hered.43/4. 205-208 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Sawazaki: "Deoxyribonuclease I (DNase I) typing from semen stains : low enzyme activity in vaginal fluids does not interfere with seminal DNase I typing from mixture stains" J.Forensic Sci.38/5. 1051-1062 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yasuda: "Human seminal deoxyribonuclease I (DNase I) : purification, enzymological and immunological characterization and origin" Clin.Chim.Acta. 218/1. 5-16 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] E.Tenjo: "Salivary deoxyribonuclease I polymorphism separated by polyacrylamide gel-isoelectric focusing and detected by the dried agarose film overlay method" Electrophoresis. 14/10. 1042-1044 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] R.Iida: "Kappa marker typing with high-performance liquid chromatography : identification of kappa marker specific tryptic peptide from the kappa lightchain of immunoglobulin G" J.Chromatogr.622/1. 9-12 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yasuda: "Two distinct secretory ribonucleases from human cerebrum : purification, characterization and relationships to other ribonucleases" Biochem.J.296/3. 617-625 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yasuda: "Use of antibodies against synthetic peptides for analysis of molecular multiplicity : human deoxyribonuclease I polymorphism" Electrophoresis. 15/2. 260-264 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yasuda: "Duplication of 1F and 1A2 genes in the vitamin D-binding protein (GC) system" Hum.Genet.93/3. 361-362 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Takeshita: "Detection of deoxyribonucleases I and II (DNases I and II) activities in reproductive organs of male rabbits" Int.J.Biochem.26/8. 1025-1031 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] D.Nadano: "Purification and characterization of human brain ribonuclease inhibitor" Arch.Biochem.Biophys.312/2. 421-428 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Takeshita: "Genetically polymorphic alpha-L-fucosidase (FUCA1) isozymes detected in blood plasma" Hum.Genet.94/3. 224-230 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yasuda: "Structure of the human deoxyribonuclease I (DNase I) gene : identfication of the nucleotide substitution that generates its classical genetic polymorphism" Ann.Hum.Genet.59/1. 1-15 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yasuda: "Molecular analysis of the third allele of human deoxyribonuclease I polymorphism" Ann.Hum.Genet.59/2. 139-147 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Sawazaki: "Correlation of alpha-1-antitrypsin (PI) polymorphism between blood and semen" Jap.J.Legal Med.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yasuda: "Chromosomal assignment of the human deoxyribonuclease I gene, DNASE1 (DNL1), to dand 16p13.3 using the polymerase chain reaction" Cytogenet.Cell Genet.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yasuda: "The molecular basis for genetic polymorphism of fuman deoxyribonuclease I : identification of the nucleotide substitution that generates the fourth allele" FEBS Lett.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yasuda,Toshihiro: "Use of antibodies against synthetic peptides for analysis of molecular multiplicity:human deoxyribonuclease I polymorphism" Electrophoresis. 15. 260-264 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasuda,Toshihiro: "Duplication of 1F and 1A2 genes in the vitamin D-binding protein(GC)system" Human Genetics. 93. 361-362 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takeshita,Haruo: "Detection of deoxyribonucleases I and ll(DNases I and II)activities in reproductive organs of male rabbits" International Journal of Biochemistry. 26. 1025-1031 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nadano,Daita: "Purification and characterization of human brain ribonuclease inhibitor" Archives of Biochemistry and Biophysics. 312. 421-428 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takeshita,Haruo: "Genetically polymorphic α-L-fucosidase (FUCAl)isozymes detected in blood plasma" Human Genetics. 94. 224-230 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasuda,Toshihiro: "Structure of the human deoxyribonuclease I (DNase I)gene:identification of the nucleotide substitution that generates its classical genetic polymorphism" Annals of Human Genetics. 59. 1-15 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasuda,Toshihiro: "Molecular analysis of the third allele of human deoxyribonuclease I polymorphism" Annals of Human Genetics. 59. 139-147 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sawaaki,Kazumi: "Correlation of alpha-1-antitrypsin (PI)polymorphism between blood and semen" Japanese Journal of Legal Medicine. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasuda,Toshihiro: "Chromosomal assignment of the human deoxyribonuclease I gene,DNASE1(DNL1),to 16p13.3using the polymerase chain reaction" Cytogenetics and Cell Genetics. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yasuda,Toshihiro: "The molecular basis for genetic polymorphism of human deoxyribonuclease I:identification of the nucleotide substitution that generates the fourth allele" FEBS Letters. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Postmortem concentrations of thiopental in tissues:a sudden death case." International Journal of Legal Medicine. 105. 239-241 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 安田 年博: "エンドヌクレアーゼアイソザイムの高感度活性染色法:Dried agarose film overlay(DAFO)法のDNase,RNaseアイソザイムの生化学的分析への応用" 生化学. 65. 39-42 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 澤崎 一三: "ペルオキシダーゼ標識抗体によるアガロースゲル中の免疫沈降物の増感法:ロケット免疫電気泳動法による尿トランスフェリンの定量" 科学警察研究所報告 法科学編. 46. 15-21 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Daita Nadano: "Measurement of deoxyribonuclease I activity in human tissues and body fluids by a single radial enzyme-diffusion method." Clinical Chemistry. 39. 448-452 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Daita Nadano: "Activity measurement and multiplicity detection of human secretory-type ribonuclease based on polycytidylic acid/ethidium bromide fluorescence." Analytical Biochemistry. 212. 111-116 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Survey of the association of deoxyribonuclease I polymorphism with disease." Human Heredity. 43. 205-208 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kazumi Sawazaki: "Deoxyribonuclease I(DNase I)typing from semen stains:low enzyme activity in vaginal fluids does not interfere with seminal DNase I typing from mixture stains." Journal of Forensic Sciences. 38. 1051-1062 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Human seminal deoxyribonuclease I(DNase I):purification,enzymological and immunological characterization and origin." Clinica Chimica Acta. 218. 5-16 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Etsuko Tenjo: "Salivary deoxyribonuclease I polymorphism separated by polyacrylamide gel-isoelectric focusing and detected by the dried agarose film overlay method." Electrophoresis. 14. 1042-1044 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Reiko Iida: "Kappa marker typing with high-performance liquid chromatography:identification of kappa marker specific tryptic peptide from the kappa light chain of immunoglobulin G." Journal of Chromatography. 622. 9-12 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Toshihiro Yasuda: "Two distinct secretory ribonucleases from human cerebrum:purification,characterization and relationships to other ribonucleases." Biochemical Journal. 296. 617-625 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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