| Project/Area Number |
05454277
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Osaka Medical College |
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Principal Investigator |
KAWAMURA Keishiro The 3rd dept.ofInt.Med, Osaka Medical College, 医学部, 教授 (00026832)
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| Co-Investigator(Kenkyū-buntansha) |
DEGUCHI Hirofumi The 3rd dept.ofInt.Med, Osaka Medical College, 医学部, 講師 (90131341)
KITAURA Ysushi The 3rd dept.ofInt.Med, Osaka Medical College, 医学部, 講師 (50084950)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥4,100,000 (Direct Cost: ¥4,100,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1993: ¥3,400,000 (Direct Cost: ¥3,400,000)
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| Keywords | dilated cardiomyopathy / myocardial biopsy / myocarditis / coxsackie B3 virus / polymerase chain reaction / in situ hybridization / PCR-SSCP / in situ PCR / 生検心筋 / in situ PCR / 電顕ISH / 心筋炎動物 / エンテロウイルス |
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| Research Abstract |
To study the viral etiology of dilated cardiomyopathy (DCM), we examined for the presence of enteroviral RNA in myocardial biopsies from patients with DCM,myocarditis (MC), or other cardiac diseases, and myocardial tissues of animal models of coxsackie B3 virus (CVB3) myocarditis, using polymerase chain reaction (PCR) and in situ hybridization (ISH).
For the clinical study, myocardial biopsies from 154 patients were examined for the presence of enteroviral genome in NC,NS and POL region by RT-PCR.The positive incidence was 20% and there was no significant difference among DCM,MC and other cardiac diseases. The genome-positive biopsies did not always show histologic findings of MC.By PCR-SSCP method enteroviral genomes were differentiated for their genotypes, and most of them were not corresponded with those of coxsackievirus group B.
In animal models of coxsackie B3 virus myocarditis, the viral genome was detected up to the 28th day of inoculation in C3H/He mice, whereas in A/J mice it was detected until the 56th day. Both strains of mice had severe and acute myocarditis and A/J mice left active myocarditis longer period that that in C3H/He mice. In hamsters with CVB3 MC the genome was present in the heart at the 6th month and some had developed DCM-like features.
In ISH the genome was located in some myocardial cells in and around the myocarditic lesions at the acute stage. By in situ PCR the genome was detected more sensitively but the method was not apropriate for histological examination because the myocardial tissue was damaged. Electron microscopic ISH disclosed ultrastructural localization of the viral genome in myocardial tissues of the animals.
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