| Project/Area Number |
05454318
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
内分泌・代謝学
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| Research Institution | Kanazawa University |
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Principal Investigator |
MIYAMORI Isamu Kanazawa University, School of Medicine, Associate Professor, 医学部, 助教授 (40142278)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEDA Yoshiyu Kanazawa University, College of Medical Technics, Assistant professor, 医療技術短期大学部, 講師 (90242544)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥700,000 (Direct Cost: ¥700,000)
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| Keywords | Aldosterone / Molecular Biology / Hyperplasia / Angiotensin / 血管内皮細胞 / P450aldo / アンジオテンシンII / 血管平滑筋 |
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| Research Abstract |
In the current study, we identified that aldosterone, a potent mineralocorticoid which is synthesized exclusively in the adrenal cortex, is also produced in the vascularture. In the first year, we undertook a perfusion experiment of the rat mesenteric arteries and identified an aldosterone like immunoreactivity in the HPLCseparated perfusate. This fraction was further analyzed by GCMS which confirmed that immunoreactive peak was identical to aldosterone molecule. In the second year, we performed gentic expression of P450aldo (aldosteone synthetase) mRNA in human endothelical cells. P450aldo mRNA was detected at a concentration of 1/50 compared with the adrenal gland. We hypothesized that vascular aldosterone may contribute to the vascular remodelling and to the pathogensis of hypertension in autocrine/paracrine fashion
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