• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular and Pathological Analysis of Anticoagulant Heparan Sulfate Proteoglycan from Endothelial Cell

Research Project

Project/Area Number 05454330
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Hematology
Research InstitutionNagoya University

Principal Investigator

SAITO Hidehiko  Nagoya University School of Medicine, Professor, 医学部, 教授 (20153819)

Co-Investigator(Kenkyū-buntansha) 松岡 明  名古屋大学, 医学部, 医員
KOJIMA Tetsuhito  Nagoya University School of Medicine, Assistant Professor, 医学部, 助手 (40161913)
TANIMOTO Mitsune  Nagoya University School of Medicine, Assistant Professor (10240805)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥5,900,000 (Direct Cost: ¥5,900,000)
KeywordsEndothelial cell / Heparan Sulfate / Proteoglycan / Ryudocan / cDNA / Immuno-histochemistry / Inhibition radioimmuno assay / 血管内皮 / alternative polyadenylation / ヒト染色体20q12
Research Abstract

We have isolated a cDNA of the human ryudocan core protein encoding a 2,610 bp transcript, which potentially codes for a 198 amino acid protein. Comparison of the deduced core proteina between the human and the rat ryudocan revealed that they have high structural conservation, particularly in the NH_2 and COOH terminus regions of the putative mature core protein, which might serve important roles for biological function of ryudocan. A major 2.7kb transcript was detected in all tissues tested, with relatively high levels of expression observed in mRNA from lung, liver, skeletal muscle and kidney. A minor 1.9 kbtranscript was also observed in some of tissues, which would be caused by alternative polyadenylation. Human ryudocan gene has localized on the chromosome 20q12 by fluorescence in situ hybridization. Immuno-histochemical analysis using a specific polyclonal antibody against human ryudocan core protein revealed that ryudocan was expressed in trophoblasts of placental villi and in endothlial cells of neovesseles, but not in those of normal vesseles. Isolation of the human ryudocan core protein cDNA will allow us to study for the regulation of ryudocan expression and the role of this molecule in human endothelial cell function

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Hidehiko Saito et al.: "Human Ryudocan Core Protein:Molecular Cloning and Chavacteriqation of the cDNA,and Chromosomal Localiqation of the Gene." Biochem.Biophys.Res.Commun.190. 814-822 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hidehiko Saito et al.: "Human Ryudocan Core Protein : Molecular Cloning and Charaterization of the cDNA,and Chromosomal Localization of the Gene" Biochem.Biophys.Res.Commun. 190. 814-822 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hidehiko Saito et al.: "Human Ryudocan Core Protein:Molecalar Cloning and Charactevigatin of the cDNA,and Chromasonal Lacol:gatien of the Gene" Biochen.Biophys.Res.Commun.190. 814-822 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kojima,T.: "Human ryudocan core protein:molecular cloning and charcterization of the cDNA,and chromosomal localization of the gene." Biochem Bioph Res Comm. 190. 814-822 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamazaki,T.: "A phenotypically neutral dimorphism of protein S:the substitution of Lys 155 Glu in the second EGF domain predicted by and a to G base exchange in the gene." Thromb Res. 70. 395-403 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Matsushita,T.: "Construction and its expression of a new retroviral vector containing a human blood coagulation factor IX cDNA." Thromb Res. 69. 387-393 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamamoto,K.: "Impaired secretion of the elongated mutant of protein C(protein C-Nagoya):Molecular and cellular basis for hereditary protein C deficiency." J Clin Invest. 90. 2439-2446 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yamamoto,K.: "Homozygous protein C deficiency:indentification of a novel missense mutation that causes impaired secretion of the mutant protein C." J Lab Clin Med. 119. 87-95 (1992)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sugiura,I.: "Three distinct point mutations of the von Willebrand factor gene in four patients with type IIA von Willebrand disease." Thromb Haemost. 67. 612-617 (1992)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi