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Functional deficiency of pulmonary surtactant in the newborn infant

Research Project

Project/Area Number 05454346
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Embryonic/Neonatal medicine
Research InstitutionSaitama Medical School

Principal Investigator

OGAWA Yunosuke  Saitama Medical School Professor, 医学部, 教授 (90080126)

Co-Investigator(Kenkyū-buntansha) ITAKURA Yukino  Saitama Medical School Assistant, 医学部, 助手 (70223071)
KANEKO Kouji  Saitama Medical School Assistant, 医学部, 助手 (30224596)
ARAKAWA Hiroshi  Saitama Medical School Assistant, 医学部, 助手 (90271238)
NAKAMURA Toshihiko  Saitama Medical School Assistant, 医学部, 助手 (30255137)
SHIMUZU Hiroshi  Saitama Medical School Lecturer, 医学部, 講師 (90260843)
小山 典久  埼玉医科大学, 医学部, 講師 (00178394)
大浜 洋一  埼玉医科大学, 医学部, 助手 (10233248)
高崎 二郎  埼玉医科大学, 医学部, 助手 (30197082)
江口 秀史  埼玉医科大学, 医学部, 講師 (30176763)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1995: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1994: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥3,200,000 (Direct Cost: ¥3,200,000)
KeywordsPulmonary surfactant / Functional deficiency / Newborn infant / Meconuum aspiration syndrome / Hemorrhagic lung edema / Airway aspirate / Tracheobronhial lavage / Pulmonary surfactant protein A / 活性阻害 / 最小表面張力 / サーファクタントアポ蛋白A / ARDS / 呼吸窮迫症候群 / 気道洗浄
Research Abstract

Pulmonary surfactant dificiency is represented by respiratory distress syndrome of the newborn infant. The similar pathophysiology should be observed where the pulmonary surfactant is inactivated by certain inhibitors. We therefore performed both animal and clinical experiments in order to clarify the pathophysilogy of functional deficiency of pulmonary surfactant and affempted to estalish the straategy for the treatment.
We first demonstrated the strong inhibitory activity of meconium on pulmonary surfactant, and succeeded in the kproduction of rabbit model of meconium aspiration syndrome. Trials of bronchoalveolar lavage with surfactant solution revealed that the bronchoalveolar lavage with surfactant solution followed by the surfactant repacement showed the best results in the removal of meconium from the airway as well as in pulmonary funciton. Clinical trials on the sick newborn infants showed the definitely promising results in the establishment of therapeutic procedure of meconiu … More m aspiration syndrome.
Then we searched for the procedure to detect functional deficiency of pulmonary surfactant. The stable microbubble rating, most widely used for the deteciton of surfactant deficiency in the neonate, was tested for functional deficiency with the addition of the serum to the sample of tracheobrochial aspirate. The miumum surface tension measured by the pulsating bubble surfactometer could detect the inhibitory activity of the serum proteins, but the stable microbubble rating was unable even when the definite amounts of inhibitor were added.
On the contrary, studies with tracheobronchial aspirates from the patients with hemorrhagic lung edema, another representative condition suspected as functional deficiency of pulmonary surfactant, revealed that the ratios of disaturated phophatidvlcholine (DSPC) to albumin and of surfactant associated protein A (SP-A) to albumin were reversely correlated to the inhibitory activity estimated by the minimum surface tension of the sample added with 1mg of artificial surfactant (S-TA) Clinical oservation also showed that the ratio of pre-and post- surfactant replacement ventilatory index indicates the inhibitory activity because the ratio wellcorrelates with the minumum surface tension of tracheobronchial aspirate samples added with the same volume of standard surfactant solution. Less

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] 板倉敬乃、大浜洋一、小山典久、江口秀史、小川雄之亮: "家兎胎便吸引症候群モデルにおける人工サーファクタント洗浄補充の呼吸機能に対する効果" 日本未熟児新生児学会雑誌. 5. 333-338 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 板倉敬乃、大浜洋一、小山典久、江口秀史、小川雄之亮: "家兎胎便吸引症候群モデルにおける人工サーファクタント洗浄補充の呼吸機能に対する効果" 日本未熟児新生児学会雑誌. 5. 333-338 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ohama,Y.,Itakura,Y.,Koyama,N.,Eguti,H.,Ogawa,Y.,: "Effect of surfactant Lovage in a rabbit model of meconium aspiration syndrome." Acta Paediatrica Japonica,. 36. 236-238 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 大浜、洋一、小山典久: "肺サーファクタントによる気道洗浄療法" 周産期医学. 25. 973-975 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 陳颯英,網塚貴介,大浜洋一,清水浩,江口秀史,小川雄之亮,小山典久,: "肺表面活性物質に及ぼす血清の影響について-Stable microbubble Stable microbubble ratingとpulsating bubble sarfactometerによる検討-" 日本未熟児新生児学会雑誌. 7. 285-289 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yunosuke Ogawa,Yoichi Ohama,Yukino Itakura,Hiroshi Shimizu,Koji Kaneko: "Bronchial Iavage with surfactant solution for the treatment of meconium aspiration syndrome" Journal of Japanese Medical Society for Biological Interface. 26(In press). (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Itakura Y,Ohama Y,Koyama N,Eguchi H,Ogawa Y.: "The effect of surfactant lavage and supplement on the pulmonary function in rabbit model of meconium aspirationi syndrome" J Jpn Soc Newborn Premature Med. 5 (2). 333-338 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ohama Y,Eguchi H,Ogawa Y.: "Inhibitory fraction (S) of meconuum against pulmonary surfactant" J Jpn Med Soc Biol Interface. 25 (1,2). 161-162 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ohama Y,Itakura Y,Koyama N,Eguchi H,Ogawa Y.: "Effect of surfactant lavage in a rabbit model of meconium aspiration syndrome" Acta Paediatr Jpn. 36. 236-238 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ohama Y,Ogawa Y.: "Airway lavae therapy with artificial surfactant solution" Perinatal Medicine. 25 (7). 973-975 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Chen S,Amizuka T,Ohama Y,Shimizu H,Koyama N, Eguchi H,Ogawa Y.: "Effects of serum on surface activity evaluated by stabel microbuble rating and pulsating bubble surfactometer" J Jpn Soc Newborn Premature Med. 7 (2). 285-289 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ogawa Y,Ohama Y,Itakura Y,Shimizu H,Kaneko K.: "Bronchial lavage with surfactant solution for the treatment of meconium aspiration syndrome" J Jpn Med Soc Biol Interface. 26 (Supplement) (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Amizuka T,Shimizu H,Chen S,Eguchi H,Niida Y. : Ogawa Y.: "Tracheal aspirates from patients with hemorrhagic lung edema exhibit inhibitory effect on the surface tension of surfactant-TA" J Jpn Med Soc Biol Interface. 27 (1,2) (in press). (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ogawa,Y.: "Target diseases for the newly developed technology in respir atory care" Pediatric Pulmonology. 11. 102-103 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 大浜洋一,小川雄之亮: "肺サーファクタントによる気道洗浄療法" 周産期医学. 25. 973-975 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 陳颯英,網塚貴介,大浜洋一,清水浩 江口秀史,小川雄之亮 小山典久,: "肺表面活性物質に及ぼす血清の影響について -Stable microbubble ratingとpulsating bubble surfactometerによる検討-" 日本未熟児新生児学会雑誌. 7. 285-289 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yunosuke Ogawa, Yoichi Ohama, Yukino Itakura, Hiroshi Shimizu, Koji Kaneko: "Bronchial lavage with surfactant solution for the treatment of meconium aspiration syndrome" Journal of Japanese Medical Society for Biological Interface. 26(In press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小川雄之亮: "新生児学 第9章 新生児疾患 2.呼吸器" メディカ出版, 37 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 小川 雄之亮: "新生児呼吸障害-古い疾患の新しい問題-" 日本新生児学会雑誌. 30. 62-65 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小川 雄之亮: "未熟児の慢性肺障害" NEW MOOK小児科. 5. 95-104 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 金子広司・小川雄之亮: "呼吸窮迫症候群(RDS)" NICU. 7. 178-182 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ohama,Y.,Itakura,Y.,Koyama,N.,Eguchi,H.,Ogawa,Y.,: "Effct of surfactant lavage in a rabbit model of meconium aspiration syndrome" Acta Paediatrica Japonica. 36. 236-238 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小山典久 中村利彦 大浜洋一 高崎二郎、他: "出生後因子からみた慢性肺疾患の病因と病態" 日本未熟児新生児学会雑誌. 6. 62-68 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 大浜洋一、江口秀史、小川雄之亮: "胎便中の肺サーファクタント阻害因子に関する検討" 日本界面医学会雑誌. 25. 161-162 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小山典久,高崎二郎,田中太平,江口秀史,神谷賢二,小川雄之亮: "新生児における成人型呼吸窮迫症候群(ARDS)" 周産期医学. 23. 540-544 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小山典久,高崎二郎,田中太平,江口秀史,神谷賢二,小川雄之亮: "新生児のARDS-病態を中心に" 日本小児呼吸器疾患学会雑誌. 4. 64-66 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 大浜洋一,小川雄之亮: "人工サーファクタントを用いた気管内洗浄療法" NICU. 6. 951-955 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 板倉敬乃,大浜洋一,小山典久,江口秀史,小川雄之亮: "家兎胎便吸引症候群モデルにおける人工肺サーファクタント洗浄補充の呼吸機能に対する効果" 日本未熟児新生児学会雑誌. 5. 333-338 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tanaka,T.,Itakura,Y.,Ohama,Y.Koyama,N.,Ogawa,Y.: "Leukotrienes in bronchial lavage fluid from infants with various neonatal respiratory diseases" Proceedings of the 2nd World Congress of Perinatal Medicine. 698-704 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ohama,Y.,Itakura,Y.,Tanaka,T.,Koyama,N.,Ogawa,Y.: "Effect of surfactant lavage in a rabbit model of meconium aspiration syndrome" Proceedings of the 2nd World Congress of Perinatal Medicine. 1129-1132 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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