| Project/Area Number |
05454351
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General surgery
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
TANI Tohru Shiga university of Medical Science First Department of Surgery, Assistant professor, 医学部, 助手 (20179823)
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| Co-Investigator(Kenkyū-buntansha) |
ENDO Yoshihiro Shiga university of Medical Science First Department of Surgery, Assistant profe, 医学部, 助手 (40263040)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1996)
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| Budget Amount *help |
¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1995: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1994: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1993: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Sepsis / Toxic Shock Syndrome Toxin-1 (TSST-1) / Peptidoglycan / glucan / Gram positive infection / グラム陽性菌 / MRSA / anti TSST-1抗体 / Gram positive bacteria / Sepsis / Toxic shock syndrom toxin-1(TSST-1) / TSST-1 |
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| Research Abstract |
The aim of this study is the establishment of rapidly diagnostic method for the gram positive bacterial sepsis. Toxic Shock Syndrome Toxin-1 (TSST-1) is one of the exotoxins produced by Staphylococcus aureus. TSST-1 has a strong potency to induce cytokines and causes toxic shock syndrome. We could establish to detect TSST-1 by ELISA method. Its normal range was less than 30pg/ml in human plasma. There was no cross reaction between Staphylococcal enterotoxin A,B and C.TSST-1 positive was defined more than 100pg/ml in plasma sample. In clinical study, TSST-1 positive rate was 65% in severe sepsis patients. Besides, endotoxin positive rate was 85%. Therefore immunosuppressive situation, it was suggested that polymicrovial infection might frequently occur.
While peptidoglycan, one of the wall compornents of microorganisms, could be detected using Silkworm Larvae plasma. Its normal range was less than 100pg/ml in human plasma. Besides, this method can detect beta-glucan (fungus compornent). Peptidoglycan positive is defined more than 100pg/ml in plasma sample. In clinical study, peptideglycan positive rate was 82.1% in severe sepsis. Combinative use of these methods including limulus endotoxin assay, contributes the early and repid diagnosis of not only both systemic gram positive and negative bacterial sepsis but also fungal inflection.
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