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Molecular biological approach for a dignasis and me chanism of sepsis

Research Project

Project/Area Number 05454364
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General surgery
Research InstitutionKeio University

Principal Investigator

UEDA Masakazu  Keio Univ., Surglry Instructar, 医学部, 助手 (50142419)

Co-Investigator(Kenkyū-buntansha) KARAHASHI Tsuyoshi  Keio Univ., Surglry Instructar, 医学部, 助手 (20245509)
MURAYAMA Yosihiko  Keio Univ., Surglry Instructar, 医学部, 助手 (30239553)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1993: ¥5,300,000 (Direct Cost: ¥5,300,000)
KeywordsSepsis / Severe Infection Dis. / MRSA / Cytokine on RNA / endothelin / PCR / MRSE
Research Abstract

We established a rapid and sensitive diagnostic method for methicillinresistant Staphylococcus aureus (MRSA) bacteremia in postoperative patients using nested polymerase chain reaction.mecA and TSST-1 genes were not detected in the blood samples of any of the 6 normal volunteers, which were negative by blood culture.In all the 12 samples from which MRSA was isolated by blood culture, mecA and TSST-1 genes were detected.In one sample from which S.epidermidis colonies grow after blood culture , mecA was detected but TSST-1 gene was not.Of 28 samples with blood cultures negative for MRSA,2 samples were still positive for mecA and TSST-1 genes.In these 2 patients, MRSA was isolated from specimens other than blood by culture.Although it took at least 48hr to identify MRSA by blood culture method, the presence of mecA and TSST-1 genes was determined by nested PCR method within only 3-4 hr after blood sampling.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] Aiura: "Circulating levels and physiological role of atrial natriurotic peptide during endotoxin shock in the rat." Critical Care Medicine. in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 市東昌也: "mRNAレベルおよび血中濃度の変化からみた手術侵襲下におけるIl-1,TNFの活性化に関する検討" 外科と代謝・栄養. 28. 331-335 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 守瀬善一: "ラットエンドトキシンショックモデルにおける内因性血中ET-1上昇に対するET変換酵素阻害剤の効果" 外科と代謝・栄養. 28. 275-280 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 小林宏行: "エドバコマブ(E5)のグラム陰性菌敗血症に対する臨床第II相試験" 感染症学雑誌. 68. 81-115 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Morise: "Pathophysiologic role of endothelin-1 in renal funchion in rats with endotoxin shock." Surgery. 115. 199-204 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 石引久弥: "MRSA感染症と対策" 消化器外科. 16. 1103-1111 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 石引久弥: "外科臨床ハンドブック(1)周術期感染の対策" 中山書店, 275 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 北川雄光: "外科臨床の分子生物学・感染症のDNA診断" 中山書店, 295 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ishibiki et.al.: "MRSA infection and treatment" Gastroenterological surgery. 16. 1103 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ishibiki et.al.: "Treatment for perisurgical patients." Handbook of surgical clinic. 275 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kitagawa et.al.: "DNA diagnosis for infections disease." Molecular biology in surgical clinics.295 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Morise et.al.: "Pathophysiological role of endothelin-1 in renal function in rats with endotoxin shock." Surgery. 115. 199 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shito et.al.: "Activation of IL-1 and TNF genes by surgical stress" J.J.Surgical Met.Nut.28. 331 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Morise et.al.: "Effect of phophoramidon on endogenous ET-1 in rat endotoxin shock model" J.J.Surgical Met.Nut.28. 275 (1004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Aiura et.al.: "Circulating levels and physiological role of atreal natriuretic peptide during endotoxin shock in the rat." Critical Care Med.(in perss).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] AIURA: "Cirulating levels and physiological role of atrial natriuretic peptide during endotoxic shock in the rat." Critical Care Medicine. in press.

    • Related Report
      1994 Annual Research Report
  • [Publications] 市東 昌也: "mRNAレベルおよび血中濃度の変化からみた手術侵襲下におけるIL-l,TNFの活性化に関する検討" 外科と代謝・栄養. 28. 331-335 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 守瀬 善一: "ラットエンドトキシンショックモデルにおける内因性血中ET-1上昇に対するET変換酵素阻害剤の効果" 外科と代謝・栄養. 28. 275-280 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 若林 剛: "重症患者の病態生理-ホルモン・サイトカインの面から-" 集中治療. 6. 663-670 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 小林 宏行: "エドバコマブ(E5)のグラム陰性菌敗血症に対する臨床第II相試験" 感染症学雑誌. 68. 81-115 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Morise: "Pathophysiological role of endothelin-1 in renal function in rats with endotoxin shock" Surgery. 115. 199-204 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 北島 政樹: "外科臨床の分子生物学:分子生物学と臨床医学、外科の接点" 中山書店, 298 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 北川 雄光: "外科臨床の分子生物学:感染症のDNA診断" 中山書店, 298 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 上田政和: "消化器癌における分子生物学的パラメータの予後因子としての有用性21GC01:日本消化器外科学会雑誌" 26. 2494-2498 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 上田政和: "担癌生体の代謝変動" JJPEN. 15. 603-307 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 石引久弥: "MRSA感染症と対策" 消化器外科. 16. 1103-1111 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Morise: "Pathophysiologic role of endothelin-1 in renal function in endotosin rats" Surgery. 115. 199-204 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tanabe: "The effect of acute lung injury and coexisting disorders on plasma lecels of atrial notriuretic peptide" Critical Care Medicine. (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] 石引久弥: "外科臨床ハンドブック(1)周術期感染の対策" 中山書店, 275 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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