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Specific active immunotherapy with anti-idiotype monoclonal antibody in lung cancer patients

Research Project

Project/Area Number 05454381
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Thoracic surgery
Research InstitutionCHIBA UNIVERSITY

Principal Investigator

FUJISAWA Takehiko  Chiba Univ.Sch.Med., Associate professor, 医学部, 助教授 (80110328)

Co-Investigator(Kenkyū-buntansha) IIZASA Toshihiko  Chiba Univ.Sch.Med., Research Instructor, 医学部, 助手 (10272303)
SAITOH Yukio  Chiba Univ.Sch.Med., Research Instructor, 医学部, 助手 (60261905)
SHIBA Mitsutoshi  Chiba Univ.Sch.Med., Research Instructor, 医学部, 助手 (20162620)
BABA Masayuki  Chiba Univ.Sch.Med., Research Instructor, 医学部, 助手 (00143305)
YAMAGUCHI Yutaka  Chiba Univ.Sch.Med., Professor, 医学部, 教授 (80009448)
MITSUNAGA Shin-ichri  Chiba Univ.Sch.Med., Research Instructor
川野 裕  千葉大学, 医学部附属病院, 助手 (40251160)
田宮 敬久  千葉大学, 医学部附属病院, 助手 (10251167)
斎藤 博明  千葉大学, 医学部, 助手 (40186953)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1995: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1993: ¥4,400,000 (Direct Cost: ¥4,400,000)
KeywordsLUNG CANCER / SURGERY / ANTI-IDIOTYPE ANTIBODY / IMMUNOTHERAPY / ADJIVANT THERAPY / CELLULAR IMMUNITY / HUMORAL IMMUNITY / ANTIGEN SPECIFICITY / 血管浸潤 / リンパ管浸潤 / 免疫能 / 腫瘍 / モノクローナル抗体
Research Abstract

Internal image anti-idiotype antibodies are expected to enhance anti-cancer effector mechanisms in vivo. We established hybridomas producing anti-idiotype monoclonal antibodies against a human monoclonal antibody (HuMoAb) 4G12 that reacts strongly with lung squamous cell carcinomas. Two clones reacted with 4G12 HuMoAb, but not with 3H12 IgM HuMoAb, human IgM,human serum or fetal calf serum. These two Ab2 antibodies (IgG1 kappa), 2B12 and 2H1 demonstrated 91.5% and 90.3% inhibition in their reactivity with radiolabelled 4G12 on PC10 cells, indicating that 2B12 and 2H1 antibodies were of Ab2 beta type. In crisscross inhibition assay, the binding of 2B12 or 2H1 to 4G12 was not inhibited by 2H1 or 2B12. Thus 2B12 and 2H1 were thought to recognize the different epitopes on the antigen binding sites. Antisera against 2B12 and 2H1 demonstrated specific reactivity to PC10 cells, confirming that 2B12 and 2H1 express internal images of lung squamous cell carcinoma recognized by the 4G12 antibody.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] 斎藤博明,他: "ヒト抗腫瘍モノクローナル抗体に対す抗イデァオタイプ抗体の作製" 臨床胸部外科. 14. 313-316 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 藤澤武彦,他: "肺癌患者の加齢に伴う免疫能低下と免疫賦活の意義" 日本呼吸器外科学会雑法. 8. 565-570 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 藤澤武彦: "抗イディオタイプモノクローナル抗体による肺非小細胞癌の診断と治療に関する検討" 病理生理. 14. 195-160 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 藤澤武彦,他: "肺癌の診断と治療の基本戦略-組織型病期の診断は治療、予後にどう関連するか" Medical Practice. 11. 820-827 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 藤澤武彦,他: "肺非小細胞,癌切除例における肺内転移と予後に関する検討" 肺癌. 35. 247-252 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takehiko Fujisawa, et al.: "Blood and lymphatic Uessel inucsion asprognostic factors for patients with primany resected nonsmall cell carcinoma of the lung with in trmpulmoncry metastasy" Cancer. 76. 2464-2470 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Huriaki Saito, et al.: "Establishment of anti-idiotype monoclonal antibodies to human anti lung cancer monoclonal antibody (4G12)." Japanese Annals of Thoracic Surgery. 14-4. 313-316 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takehiko Fujisawa, et al.: "Decrease of immunity associated with aging and the significance of immune activation in lung cancer patients" Japanese Journal of Chest Surgery. 8-5. 565-570 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takehiko Fujisawa, et al.: "Strategy of diagnosis and treatment of lung cancer." Medical Practice. 11-5. 820-827 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takehiko Fujisawa: "Diagnosis and treatment of non-small cell carcinomas using anti-idiotype monoclonal antibody" Biopathology. 14-2. 159-160 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takehiko Fujisawa, et al.: "Prognosis in primary, resected non-small cell carcinoma cases with intrapulmonary metastasis" Japanese Journal of Lung Cancer20GD05 : 35-3. 247-252 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takehiko Fujisawa, et al.: "Blood and lymphatic vessel invasion as prognostic factors for patients with primary resected nonsmall cell carcinoma of the lung with intrapulmonary metastasis" Cancer. 76. 2464-2470 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 斎藤博明他: "ヒト抗肺癌モノクローナル抗体に対する抗イディオタイプ抗体の作製" 臨床胸部外科. 14. 313-316 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 藤澤武彦他: "肺癌患者の加齢に伴う免疫能低下と免疫賦活の意義" 日本呼吸器外科学会雑誌. 8. 565-570 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 藤澤武彦: "抗イディオタイプモノクローナル抗体による肺非細胞癌の診断と治療に関する検討" 病態生理. 14. 159-160 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 藤澤武彦他: "肺癌の診断と治療の基本戦略-組織型、病期の診断は治療.予後にどう関連するか" Medical Pracfice. 11. 820-827 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 藤澤武彦他: "肺非小細胞癌切除例における肺内転移と予後に関する検討" 肺癌. 35. 247-252 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takehiko Fujisawa et al: "Blood and lymphqtic vessel invasion as prognostic factors for potients with primaryresected nonsmall cell carcinoma of the long with intrapulmonary inetastasis" Concer. 76. 2464-2470 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 斉藤博明: "ヒト抗肺癌モノクローナル抗体に対する抗イディオタイプ抗体の作製" 臨床胸部外科. 14. 313-316 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 藤澤武彦: "抗イディオタイプ・モノクローナル抗体による肺非小細胞癌の診断と治療に関する検討" 病態生理. 14. 159-160 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 藤澤武彦: "肺癌の診断と治療の基本戦略" Medical Practice. 11. 820-827 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 藤澤武彦: "肺癌の加齢に伴う免疫能低下と免疫賦活の意義" 日本呼吸器外科学会雑法. 8. 565-570 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 藤澤武彦: "高齢者肺癌に対する外科治療の問題点" 呼吸. 12. 470-474 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 藤澤武彦: "老人者肺癌-診断と治療の進歩" 現代医療. 25. 2277-2281 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 深澤敏男: "特異的免疫療法を目的としたヒト抗肺癌monoclonal抗体(4G12)に対する抗idiotype抗体(Ab2β)の研究" 日本外科学会雑誌. 93 (総会抄録号). 237 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 斎藤博明: "ヒト抗肺癌モノクローナル抗体に対する抗イディオタイプモノクローナル抗体の作製" 日本胸部外科学会雑誌. 41. 1803 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2018-02-02  

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