| Project/Area Number |
05454411
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
KUBO Toshikazu Kyoto Prefectural University of Medicine, Orthopaedic Surg, Associated Prof., 医学部, 助教授 (20178031)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1994: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1993: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | Osteoarthritis / Heat shock protein / Hydrostatic pressure / PCR / Cytokine / Black mouse / 免疫組織化学 / in situ hybridization |
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| Research Abstract |
1. Localization of heat shock protein in osteoarthritic cartilage : by immunohistochemistry, in normal and mild osteoarthritic cartilage, HSP70 was only localized in the chondrocytes of the superficial layr where safranin O staining was reduced. In moderate and severe osteoarthritic cartilage, the chondrocytes with positive staining were recognized to deeper layrs. The distribution of positively stained chondrocytes was correlated to the severity of osteoarthritis (OA).
2. Influence of hydrostatic pressure (HP) on chondrocytes : Chondrocyte like cells (HCS2/8) were subjected to HP treatment ranging from 0Mpa to 50Mpa. Expression of bFGF and IGF-I mRNA was enhanced by HP as shown by PCR analysis. Expression of TGF beta1 mRNA was enhanced after 5Mpa HP and reduced after the 50 Mpa HP.Expression of HSP70 mRNA was markedly enhanced after 50 Mpa HP.Incorporation of 35S sulfate after 5 Mpa HP treatment increased significantly compared with the control. However, it was reduced after 50 Mpa HP treatment.
3. Analysis of spontaneous osteoarthritic mouse by PCR : Six-month-old C57 black mouse (Silberberg) which is developing spontaneous OA were used. Total RNA was extracted from the proximal tibial cartilage and PCR for various cytokines and HSPs was performed. Statistical analysis indicated that IL-6, IFN-gamma and HSPs (47,70,86) were strongly expressed in comparison with control mouse.
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