| Project/Area Number |
05454416
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Anesthesiology/Resuscitation studies
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| Research Institution | UNIVERSTY OF TOKYO |
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Principal Investigator |
HANAOKA Kazuo Univ.of Tokyo (Faculty of Medicine), Anesthesiology, Professor, 医学部(病), 教授 (80010403)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAMURA Hideo Univ.of Tokyo (Faculty of Medicine), Anesthesiology, Professor Emeritus, 医学部(病), 名誉教授 (60009884)
IDE Yasuo Univ.of Tokyo (Faculty of Medicine), Anesthesiology, Instructor, 医学部(病), 助手 (60193463)
SUMIDA Toshinobu Univ.of Tokyo (Faculty of Medicine), Anesthesiology, Instructor, 医学部(病), 助手 (80187806)
TAGAMI Megumi Tokyo University Branch Hospital, Anesthesiology, Associated Professor, 医学部(分), 助教授 (90107657)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1993: ¥5,500,000 (Direct Cost: ¥5,500,000)
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| Keywords | spinal cord / single-unit activity / dorsal horn / wide dynamic range (WDR) cell / hyperventilation / pinching stimulus / PaCO_2 / extracellular microelectrode measurement / wide dynamic range / 脊髄後角単一細胞 / Wide dynamic range neuron / 自発発射 / 誘発発射 / ペアン鉗子ピンチ法 / Paco_2 |
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| Research Abstract |
The purpose of this study is to investigate the effects of hyperventilation on the wide dynamic range (WDR) cell of the dorsal horn of the feline lumbar spinal cord.
Under enflurane anesthesia, cats were prepared with midcollicular decerebration and lumbar laminectomy. The spinal cord was transected at T12-L1. WDR cells, responding primarily to noxious peripheral stimuli, were sampled with a microelectrode at the depth of 1500-2000 um from the cord dorsum. Spontaneous activity and noxiously evoked activity by pinching the receptive field on the hind paw were measured.
Two series of experiments were carried out to characterize the effects of hyperventilation. In the first, following the control period with PaCO_2 of 35-45 mHg, respiratory rate and tidal volume were adjusted to make a level of hypocapnia as low as 25-30 and 20-25 mmHg. The recovery of cell activity was followed when normocapnia was restored. In the second, after activities of WDR cells were well suppressed by hypocapnia of
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PaCO_2 20-25mmHg, naloxone 0.1mg/kg or phentolamine 0.5mg/kg with normal saline 1.0 ml was given intravenously. Changes of firings of WDR cells were investigated. Retuening to normocapnia, recovery of firings was followed.
Hypocapnia with PaCO_2 of 25-30 mmHg and 20-25 mmHg suppressed the activity of WDR cell significantly. At PaCO_2 of 25-30 mmHg, spontaneous activity was suppressed for about 20 % and evoked activity for 27 %. Ar PaCO_2 of 25-30 mmHg the spontaneous activity was suppressed for. about 50 % and evoked activity for 33 %. The results suggest that hyperventilation has suppressive effects on single-unit activity of WDR cell. Both naloxone and phentolamine significantly antagonized the suppressive effects of hyperventilation upon the activity of WDR cell.
Our results suggest that hyperventilation has suppressive effects on single-unit activity of WDR cell and the mechanisms of those suppressive effects are related to not only endogeneous opiate but also adrenergic pain modulating system. Less
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