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Basic and clinical researchers for molecular diagnosis of abnormalities of testicular function

Research Project

Project/Area Number 05454433
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionOsaka University

Principal Investigator

NAMIKI Mikio  Osaka University, Dept of Urology, Associate Professor, 医学部, 助教授 (70155985)

Co-Investigator(Kenkyū-buntansha) MATSUMIYA Kiyomi  Osaka University, Dept of Urology, Assistant Professor, 医学部, 助手 (90243237)
OKUYAMA Akihiko  Osaka University, Dept of Urology, Professor, 医学部, 教授 (20093388)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1994: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1993: ¥4,500,000 (Direct Cost: ¥4,500,000)
Keywordssex maldevelopment / male infertility / androgen / receptor / FSH / 精巣機能 / mRNA / FSH / AR
Research Abstract

It is very important for diagnosis and treatment of sex maldevelopment and testicular disorders such as male intertility to clarify the control mechanism of human testicular function. In the present research, the following results were obtained by experiments with molecular biological techniques.
1. A minute deletion of Y chromosome was detected in the DNA analysis of idiopathiv interfile men. AZF should be included with the deleted vegion. Molecular cloning has narrowed down the region to Yg11.23.
2. DNA analyzes of 4 cases of complete type of testicular feminization revealed that one-point mutation was detected in exon B,C,D and H in each case, respectively. In 3 cases of 35 idiopathic infertile men, one-point mutation(at exon B,C and D,respectively) was also detected.
3. AR mRNA and FSH-R mRNA expressed in undescended testes were less than normal testes, whereas expression of inhibin mRNA was increased in undescended testes.Because, AR mRNA and FSH mRNA expressed in testes of infertile men were decreased, it is suspected that some male infertility could be caused by abnormality of testicular AR or FSH-R.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Shingo Takaba et al: "Detection of renin gene expression in human testis by reverse transcriptase-polymerase chain reaction" Moleculay Andrology. 5. 77-80 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nobuyuki Kondoh et al: "Defection of aberrations in androgen recetor gene by analysis of PCR-SSCP" Urological Research. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shingo Takada et al.: "Detection of renin gene expression in human testis by reverse transcriptase-polymerase chair reaction" Molecular Andrology. Vol 5. 77-80 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nobuyuki Kondoh et al.: "Detection of aberrations in androgen receptor gene by analysis of PCR-SSCP" Urological Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shirgo Takada et al.: "Detection of renin gene expression in human testis by reverse transcriptase-polymerase chain reaction" Molecular Andrology. 5. 77-80 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Nobuyuki Kondoh: "Detection of aberrations in androgen receptor gene by analysis of PCR-SSCP" Urological Research. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Mikio Namiki et al: "Hormonal regulation of human testicular inhibin α and βB subunit messenger RNA_s" Journal of Urology. 150. 1007-1009 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shigeo Nagafuchi: "A minute deletion of the Y chromosome in men with azoospermia" Journal of Urology. 150. 1155-1157 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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