| Project/Area Number |
05454444
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nagoya University |
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Principal Investigator |
MIZUTANI Shigehiko Nagoya University, School of Medicine OB & GY,Associate Professor, 医学部, 助教授 (00159162)
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| Co-Investigator(Kenkyū-buntansha) |
KURAUCHI Osamu Nagoya University, School of Medicine OB & GY,Lecture, 医学部, 講師 (80195528)
TOMODA Yutaka Nagoya University, School of Medicine OB & GY,Professor, 医学部, 教授 (60023769)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1995: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1994: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | placental leucine aminopeptidase (P-LAP) / oxytocin / vasopressin / somatostatin / angiotensin / angiotensinase / bradykinin / placental function / D比 / 胎盤性ロイシンアミノペプチダーゼ(P-LAP) / cDNA / オキシトシン / パゾブレシン / アミノペプチダーゼN(CD13) / スプレノペンチン / サイモペンチン / タフトシン / アミノペプチダーゼP / ヒト胎盤 / 妊娠の免疫調節 |
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| Research Abstract |
We tried to understand pathophysiology in pregnancy via interrelationship between bioactive peptide hormones and their degradation proteases in placenta.
1. Basic Research We showed the following evidences :
(1) We isolated a cDNA clone with 4084 base pairs encoding P-LAP from a human placental cDNA library. The deduced suquence contains a hydrophobic region near the N terminus, suggesting that the enzyme is a type II intergral membrane protein.
(2) The oxytocin and vasopressin degradation protease in human placenta is P-LAP.
(3) The somatostatin degradation protease in human placenta is P-LAP.P-LAP might be involved in the development of the fetus via regulation not only the concentration of somatostatin but also vasoactive peptides such as vasopressin in the feto-placental circulation.
(4) The initiating protease of angiotensin II degradation in human placental in aminopeptidase A and aminopeptidase N (AP-N).
(5) Placental AP-N (CD13) degrades immunomodulating peptides such as tuftsin, thymopentin and splenopentin. AP-N might be involved in the immunology of pregnancy via regulating the concentration of immunomodulating peptides.
2. Clinical Research We showed the following evidences :
(1) Aminopepidase P,which might degrade bradykinin, increases in pregnancy sera with advancing gestation.
(2) P-LAP in useful for monitoring post date pregnancy and premature delivery.
(3) We found the significant correlation between pulsed Doppler S/D ratio and P-LAP activity in normal pregnancy. The P-LAP activity in pregnancy sera might reflect the vascular resistance in umbilical artery via regulation the concentration of fetal vasopression.
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