| Project/Area Number |
05454458
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Hyogo College of Medicine |
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Principal Investigator |
TSUJI Yoshiyuki Hyogo College of Medicine, Obstetrics and Gynecology, Associate Professor, 医学部, 助教授 (60148658)
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| Co-Investigator(Kenkyū-buntansha) |
FURUYAMA Jyunichi Hyogo College of Medicine, Genetics, Professor, 医学部, 教授 (30068431)
SHIMA Tomoko Hyogo College of Medicine, Genetics, Associate Professor, 医学部, 助教授 (10172868)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,200,000 (Direct Cost: ¥6,200,000)
Fiscal Year 1994: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1993: ¥4,400,000 (Direct Cost: ¥4,400,000)
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| Keywords | FISH / Mosaicism / Abortion / Chromosome / Placenta / DNA / Chromoscme / 流産 / 染色体異常 |
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| Research Abstract |
Twenty seven spontaneous abortions and seven normal placental villi were examined for chromosome karyotype mosaicisms by using fluorescent in situ hybridization (FISH).
The chromosome specific digoxin labeled probes, D17 Z1 and D4Z1 were applied to collagenase digested placental villi cells. There were no significant differences among profile of signal numbers on cell nucleus in comparing between spontaneous abortus and normal villi. However there were two cases of abortus villi that contained about 10% cell that bear three signals on every nuclei, in contrast only 1-2% of cells have three signals in another cases. This was confirmed by using two chromosome probes, D17Z1 and D4Z1. Both probe revealed 3 signals cell containing about 10% of each cases. This result suggest that some spontaneous abortion have the low frequent triploid mosaicism in the villi cells.
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