| Project/Area Number |
05454461
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Osaka University |
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Principal Investigator |
DOI Katsumi Osaka University, Dept.of Otolaryngology, Assistant Professor, 医学部, 講師 (40243224)
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| Co-Investigator(Kenkyū-buntansha) |
SAKAGAMI Masafumi Hyogo Medical College, Dept.of Otolaryngology, Professor, 医学部, 教授 (10170573)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1993: ¥3,300,000 (Direct Cost: ¥3,300,000)
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| Keywords | Meniere's disease / endolymphatic hydrops / water channel / endothelin receptor / mineralocorticoid receptor / forskolin / CAMP / 内耳 / Isk / mineralcorticorid / endothelin / water channel / vasopressin / adenylate cyclase / 分子生物学 / ホルモンレセプター / K^+チャンネル |
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| Research Abstract |
To determine the molecular mechanism underlying the formation of endolymphatic hydrops in Meniere's desease, the expressions of several receptors and channels in the rat inner ear were examined by using molecular biological techniques including RT-PCR (reverse transcription-polymerase chain reaction), DNA sequencing, in-situ hybridization, and immunohistochemistry. The expressions of endothelin receptor, mineralocorticoid type I receptor, beta-adrenergic receptor, and water channels were identified. Adenylate cyclase-cyclic AMP system should be responsible for the acute endolymphatic hydrops by modulating the transport of Cl ion between the endolymphatic and perilymphatic spaces. Water channels, AQP2 and AQP3 may also be critical factors in the development of endolymphatic hydrops, because these highly water-permeable molecules are present in the lumen of the endolymphatic sac, where endolymph is believed to be absorbed relative rapidly.
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