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Periodontal Ligament as a sensory apparaus : A morphological approach to functional property of periodontal nerve terminals

Research Project

Project/Area Number 05454488
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Morphological basic dentistry
Research InstitutionNIIGATA UNIVERSITY

Principal Investigator

TAKANO Yoshiro (1994)  Niigata University, School of Dentistry Professor, 歯学部, 教授 (90126425)

前田 健康 (1993)  新潟大学, 歯学部, 助教授 (40183941)

Co-Investigator(Kenkyū-buntansha) OHSHIMA Hayato  Niigata University, School of Dentistry Research Associate, 歯学部, 助手 (70251824)
高野 吉郎  新潟大学, 歯学部, 教授 (90126425)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1993: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywordsperiodontal ligament / PGP 9.5 / Ruffini ending / development / nerve injury / histochemistry / immunohistochemistry / serotonin / Periodontal ligamert / Ruffini ending / development / nerve injury / histochenistry / immunohistocheristry / serotonvn / チロクロームオキシダーゼ / 酸性フォスフォターゼ / ルフィン神経終末 / インプラント / PGP 9.5
Research Abstract

1. Double staining with non-specific cholinesterase and protein gene product 9.5 (PGP) clearly demonstrated that mechanoreceptors are restricted to the area near root apex while nociceptors are densely distributed throughout the whole length of periodontal ligament.
2. In histochemical studies, almost all mitochondria in the axon terminals of Ruffini endings showed intense non-specific cholinesterase. Terminal Scwann cells associated with periodontal Ruffini endings were positive for acid phosphatase.
3. The development and maturation process of Ruffini endings were investigated by use of immunohistocheistry and immunocytochemistry for PGP.These processed require the additions of functional stimuli such as tooth eruption and occlusal force.
4. The responses of periodontal nerves to experimentally induced occlusal trauma in rat molars were assessed by immunohistochemistry for PGP.By the second day after bite-raising, many Ruffini endings were swollen and their outline were unclear at light … More microscopic level. Under electron microscope, unusual long cytoplasmic projections extended through enlarged slits of Schwann cell covering, and the cavcolac of the Schwann sheaths had decreased in number. Furthermore, from days 2 to 4, thin nerve fibers on the pressure side of the periodontal ligament were oriented irregulary and had a prominet beated appearance. An increase in the beaded nerves occurred in between days 2-4 post elevation, and decreased over the time course. These results suggest celusal trauma induces specific changes in the distribution and shape of nerve terminals in the periodontal ligament.
5. For elucidation in the kinds of neuromodulater concerning jaw-reflexes, microinjection with HRP into masseter was done followed by immunocytochemistry for serotonin in cat trigeminal motor nucleus. The HRP-labelled motor neuron formed synapse with serotonin-positive nerve fibers. The results in this study suggest serotonin is one of neuromodulators for control of jaw-movement. Less

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] 澤田雅仁ほか: "インプラント周囲組織の経時的変化-特に神経線維の動態について-" 日本歯科補綴学会雑誌. 37. 144-158 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kannari K.et al.: "Sinusoidal vessels in the periodontal ligament of hamster incisors:Their distribution,structure and possible function." Arch.Histol.Cytol.56. 91-99 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Maeda t. et al.: "Cytochrome oxidase activity as a marker for periodontal sensory receptors." Archs.Oral Biol.38. 255-1993 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Maeda t. et al.: "Histochemical demonstration of acid phosphatase activity in periodontal Ruffini endigs in rat incisor." Archs.Oral Biol.38. 611-617 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakakura-Ohshima K.et al.: "Postnatal development of periodontal innervation in rat incisors:An immunohistochemical study using protein gene product 9.5 antibody." Arch.Histol.Cytol.56. 385-398 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Saito I.et al.: "Alteration of nerve growth factor-receptor expression in the periodontal ligament of the rat during expermental tooth movement." Archs.Oral Biol.38. 923-929 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Maeda T.et al.: "Evidence for the existence of intraepithelial nerve endings in the junctional epithelium of rat molars:An immunohistochemical study using protein gene product 9.5(PGP9.5)antibody." J.Periodont.Res.29. 377-385 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Maeda T.et al.: "Dense innervation in human radicular dental pulp as revealed by immunohistochemistry for protein gene product 9.5." Archs.Oral Biol.39. 563-568 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohshima H.et l.: "The relationship between odontobalsts and immunocompetent cells during dentinogenesis in rat incisors:An immunohistochemical study using OX6-monoclonal antibody." Arch.Histol.Cytol.57. 435-448 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 小林龍彰ほか: "ラット顎関節の神経支配-抗protein gene product9.5(PGP9.5)血清を用いた免疫組織化学的研究-" 日本口腔科学会雑誌. 43. 369-385 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawahara I.et al.: "Immunohistochemical characterization and Iocalization of MHC class II antigen presenting cells in the periodontal ligament of rat incisors." Connect.Tissue Res.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sawada M.et al.: "Histological investigation on chronological changes in peri-implant tissues, with special reference to responses of nerve fibers to implantation" J.Jpn.Prosthodont.Soc.37 (1). 144-158 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kannari K.et al.: "Sinusoidal vessels in the periodontal ligament of hamster incisors. Their distribution structure and possible function" Arch.Histol.Cytol.56 (1). 91-99 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Maeda T.et al.: "Cytochrome oxidase activity as a marker for periodontal sensory receptors" Archs Oral Biol.38 (3). 255-259 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Maeda T.et al.: "Histochemical demonstration of acid phosphatase activity in periodontal Ruffinien digs in rat incisors" Archs Oral Biol.38 (7). 611-617 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nakakura-Ohshima K.et al.: "Postnatal development of periodontal innervation in rat incisors : An immunohistochemical study using protein gene product 9.5 antibody" Arch Histol.Cytol.56 (4). 385-398 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Saito I.et al.: "Alteration of nerve growth factor-receptor expression in the periodontal ligament of the rat during experimental tooth movement" Archs Oral Biol.38 (11). 923-929 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Maeda T.et al.: "Evidence for the existence of intraepithelial nerve endings in the junctional epithelium of rat molars : An immunohistochemical study using protein gene product 9.5 (PGP9.5) antibody" J.Periodont.Res.29. 377-385 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Maeda T.et al.: "Dense innervation in human radicular dental pulp as revealed by immunohistochemistry for protein gene product 9.5" Archs.Oral Biol.39 (7). 563-568 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ohshima H et al.: "The relationship between odontobalsts and immunocompetent cells during dentinogenesis in rat incisors : An immunohistochemical study using OX6-monoclonal antibody" Arch.Histol.Cytol.57 (5). 435-447 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kobayashi T.et al.: "Innervation of rat temporomandibular joint -An immunohistiochemical study using protein gene product 9.t antibody-" J Jpn Stomatol.Soc.43 (3). 369-385 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawahara I.et al.: "Immunohistochemical characterization and localization of MHC class II antigen presenting cells in the periodontal ligament of rat incisors" Connect Tissue Res.(in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Maeda,T.Sodeyama,T.Hara,Y.Takano: "Evidence for the existence of intraepithelial nerve endings in the junctionaol epithelium of rat molars:an immunohistochemical study using protein gene product9./5(PGP9.5)antibody." Journal of Periodontal Research. 29. 377-385 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Maeda,S.Homma,Y.Takano: "Dense innervation of human radicular dental lpulp as revealed by immunocytochemistry for protein qene product9.5." Archives of Oral Biology. 39. 563-568 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Ohshima,I.Kawahara,T.Maeda,Y.Takano: "The relationship between odontoblasts and immunocompetent cells during dentinogenesis in rat incisors:an immunohistochemical stucy using OX^A-monoclonal antibody." Archives of Histology and Cytology. 57. 435-448 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Takano: "Histochemical aspects of calcium regulation by the enamel forming cells during matrix formation and maturation." Acta Anatomica Nipponica. 69. 106-122 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 高野吉郎: "エナメル質石灰化とその調節因子" 新潟歯学会雑誌. 24. 1-20 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Ohshima,O.Sato,I.Kawahara,T.Maeda,Y.Takano: "Responses of immunocompetent cells to cavity preparation in rat molars:An immunohistochemical study using OX6-monoclonal antibody." Connective Tissue Research. 31(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] I.Kawahara,Y.Takano: "Immunohistochemical characterization and localization of immunocompetent cell in the periodontal liqament of rat incisors." Connective Tissue Research. 31(in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] I.Kawahara,Y.Takano: "Segregated localization of immunocompetent cells and osteoclasts in the periodontal ligament of rat molar tooth." Archives of Histology and Cytology. (in press). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 澤田雅仁ほか: "インプラント周囲組織の経時的変化-特に神経線維の動態について-" 補綴誌. 37. 144-158 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Kannnari et al.: "Sinusoidal vessels in the periodontal ligament of hamster incisors:Their distribution,structure and possible function." Arch.Histol.Cytol.56. 91-99 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Maeda et al.: "Cytochrome oxidase activity as a marker for periodontal sensory receptors." Arch.Oral Biol.38. 255-259 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Maeda et al.: "Histochemical demonstration of acid phosphatase activity in periodontal Ruffini endings in rat incisors." Arch.Oral Biol.38. 611-617 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] I.Saito et al.: "Alteration of nerve growth factor-receptor expression in the periodontal ligament of the rat during experimental tooth movement." Arch.Oral Biol.38. 923-929 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Nakakura-Ohshima et al.: "Postnatal development of periodontal innervation in rat incisors:An immunohistochemical study using protein gene product 9.5 antibody." Arch.Histol.Cytol.56. 385-398 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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