| Project/Area Number |
05454491
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Showa University |
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Principal Investigator |
YAMAGUCHI Akira Showa University, School of Dentistry, Assistant Professor, 歯学部, 助教授 (00142430)
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| Co-Investigator(Kenkyū-buntansha) |
KATAGIRI Takenobu Showa University, School of Dentistry, Research Associate, 歯学部, 助手 (80245802)
SUDA Tatsuo Showa University, School of dentistry, Professor, 歯学部, 教授 (90014034)
YOKOSE Satoshi Showa University, School of Dentistry, Research Associate, 歯学部, 助手 (90245803)
IKEDA Tohru Showa University, School of Dentistry, Associate Professor, 歯学部, 講師 (00211029)
YOSHIKI Shusaku Showa University, School of dentistry, Professor, 歯学部, 教授 (30085740)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥7,400,000 (Direct Cost: ¥7,400,000)
Fiscal Year 1994: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1993: ¥5,600,000 (Direct Cost: ¥5,600,000)
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| Keywords | osteoblast / chondrocyte / muscle / BMP / 培養細胞 / In situ hybridization |
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| Research Abstract |
The skeletal mesenchymal cells including osteoblasts, chondrocytes, muscle cells and adipocytes are believed to be derived from the common mesenchymal progenitors. Using various cell lines, multipotential mesenchymal progenitors capable of differentiating into these cell lineages have been demonstrated to be present in the skeletal tissues. The developmental potential of the progenitors becomes more restricted according to their maturation stages in the specialized cell lineages. Among the various growth factors, bone morphogenetic protein (BMP), which belongs to the transforming growth factor-beta (TGF-beta) superfamily, plays an important role in the regulation of the differentiation pathway of these mesenchymal cells. BMP-2 induces the undifferentiated mesenchymal progenitors to differentiate into osteoblasts, chondrocytes and adipocytes. BMP-2 also stimulates the committed osteo-chondroprogenitors to differentiate into more mature cell types, but inhibits myogenic cells to differentiate into myotubes. BMP-2 converts the differentiation pathway of C2C12 myoblasts into the osteogenic lineage associating with decreases in the expression levels of the muscle specific regulatory factors such as myogenin and MyoD.TGF-beta inhibits myotube formation in myogenic cell lines, but dose not stimulate osteoblast differentiation. The TGF-beta superfamily plays important roles in the differentiation process of the skeletal mesenchymal cells.
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