| Project/Area Number |
05454519
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
HARA Yoshitaka Nagasaki University, School of Dentistry Associate Professor, 歯学部, 助教授 (60159100)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIMURA Atsutoshi Nagasaki University, School of Detistry Research Associate, 歯学部, 助手 (70253680)
OZAKI Yukio Nagasaki University, School of Dentistry Research Associate, 歯学部, 助手 (60204187)
TANI Masahiko Nagasaki University, School of Dentistry Research Associate, 歯学部, 助手 (70188374)
ICHIMARU Eiji Nagasaki University, School of Dentistry Research Associate, 歯学部, 助手 (60223111)
KATO Ihachi Nagasaki University, School of Dentistry Professor, 歯学部, 教授 (30005087)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1994: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1993: ¥5,900,000 (Direct Cost: ¥5,900,000)
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| Keywords | Bone resorption / T cell / Nude mouse / SCID mouse / T細胞 / 病理組織学的検索 |
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| Research Abstract |
Periodontal disease are caused by bacterial infection and some immune cells locally infiltrated against it. There cells secrete cytokines which have been reported to act not only as regulatory substances for immune responses, but mediators of tissue destruction. In fact, there have been many reports which suggest the relation between some cytokines secreted from T cell, a mediator of immune responses, and alveolar bone resorption. However, it has been unclear that T cells truly relate with periodontal tissue destruction. So in first study, alveolar bone resorption induced by every 48 h injections of E.coli endotoxin was investigated in normal mice, T cell deficient nude mice and SCID mice congenitally lacking both T and B cells. In addition, CD4 and/or CD8 depleted mice by the administration of anti-CD4 and/or anti-CD8 monoclonal antibodies were used in this study. In normal mice, alveolar bone resorption was observed after 4 times injection, and after 7 times in nude mice and 10 times in SCID mice. T cell depleted mice, especially CD4 depleted mice, also revealed the weaker and delayd bone resorption. These findings strongly suggested the relation between T cells and bone resorption. So in second experiment, mice transfered with T cells were established and injected with endotoxin as same as in first study. After 4 times injection, these mice showed the weak but obvious bone resorption similarly in normal mice. Based on these results, T cells closely related the bone resorption induced by endotoxin and further study should be investigated on cytokine production by T cells.
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