| Project/Area Number |
05454520
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
SUEDA Takeshi Kagoshima University, Department of Periodontology, Professor, 歯学部, 教授 (30013890)
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| Co-Investigator(Kenkyū-buntansha) |
MACHIGASHIRA Miho Kagoshima University, Department of Periodontology, Instructor, 歯学部, 助手 (80253897)
KUBO Kohji Kagoshima University, Department of Periodontology, Instructor, 歯学部, 助手 (00234477)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1994: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1993: ¥3,800,000 (Direct Cost: ¥3,800,000)
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| Keywords | Subgingival Plaque / LPS / Gingival Fibroblasts / Periodontal Fibroblasts |
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| Research Abstract |
Since lipopolysaccharide (LPS), outer membrane and vesicle appear to be a common factors to all gram negative bacteria that are predominant in the gingival sulcus of the sites of periodontal disease and they have well documented biological effects, they have receipt prominent attention. The purpose of this study was to compare the biological activity under the standardized condition.
We studied the effects of LPS,outer membrane and vesicle on cultured human fibroblasts. The stimulators were extracted from P.gingivalis, P.intermedia, A.actinomycetemcomitans derived from periodontal patients. Fibroblasts were obtained from healthy gingiva and healthy periodontal ligament of the same individual. The proliferation and the alkaline phosphatase activity, an osteoblastic characteristic, were compared between these two types of cell.
The results were as follows :
The proliferation was varied regularly on both cells according to the stimulated concentration. Particularly after the stimulation of P.intermedia regularity is most clear among the three bacteria and after the stimulation of vesicle is most clear among the three stimulators. It was showed similar regularity according to the stimulated concentration on both cells of alkaline phosphatase activity. Cultured fibroblasts derived from periodontal ligament have higher alkaline phosphatase activity than that of gingival fibroblasts. After stimulation, the fibroblasts were examined by electron microscopy. The cells showed a irregularity of the surface of outer membrane at many locations.
In this study, we could clearly compare the biological activity under the standardized condition. In the future, the correlation with biological activities will be defined more clearly.
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