| Project/Area Number |
05454543
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Surgical dentistry
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
SHINOHARA Masanori KYUSHU UNIVERSITY,DENTISTRY Assistant Prof., 歯学部, 講師 (90117127)
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| Co-Investigator(Kenkyū-buntansha) |
TAKENOSHITA Yasuharu KYUSHU UNIVERSITY,DENTISTRY Asociate Prof., 歯学部, 助教授 (50117157)
KUBO Shuro KYUSHU UNIVERSITY,DENTISTRY Research Asociate, 歯学部, 助手 (00205121)
NAKAMURA Seiji KYUSHU UNIVERSITY,DENTISTRYAssistant Prof., 歯学部, 講師 (60189040)
岡 増一郎 九州大学, 歯学部, 教授 (50038866)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1993: ¥3,100,000 (Direct Cost: ¥3,100,000)
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| Keywords | Lymphcyte Subset / Adhesion Molecules / T Cell Receptor Repertoire |
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| Research Abstract |
1) The TCR repertoires of TIL in oral SCC were more restricted than PBMC,but still heterogeneous. Careful comparative study revealed that certain V beta-positive T cells were frequently increased in TIL.Although they were polyclonal with different CDR3 sequences, the preferential usage of J beta gene segnents was also observed. Taken together, it is sugestthat the unique T cell subpopulations are amplified possibly as a consequence of antigen-driven selection.
2) Using the PCR-based method, cytokine mRNA expression of T cells in oral SCC was examined.Th1type cytokines such as IL-2 and IFN-gamma were more frequently detected that Th2 type cytokines such as IL-4 and IL-5. Furthermore, IFN-gamma were detected more often in patients with marked lymphocyte infiltration. These results suggest that CD4_+ Th1 cells, especially IFN-gamma producing CD4_+ T cells, may participate in active immune responses against oral SCC.
3) Extracellular matrix, MMP-1, -2, -3, -9, TIMP-1, -2, and VLA-1-6 are significantly involved in the invasion and metastasizing processes of tumour cells, and that the detection of athese factors is valuable and objective to further evaluate the invasion and metastatic potential of oral SCC,in addition to the conventional histologic grading.
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