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In situ Detection of Virus in Oral Cancer

Research Project

Project/Area Number 05454550
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Surgical dentistry
Research InstitutionTsurumi Univ.

Principal Investigator

SATO Junichi  Tsurumi University : Dental Medicine, Assistant Professor, 歯学部, 講師 (70187201)

Co-Investigator(Kenkyū-buntansha) HAMADA Yoshiki  Tsurumi University. Dental Medicine, Assistant, 歯学部, 助手 (70247336)
SETO Kanichi  Tsurumi University. Dental Medicine, Peofessor, 歯学部, 教授 (60064367)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1993: ¥5,300,000 (Direct Cost: ¥5,300,000)
KeywordsHPV. / Oral cancer / in situhybridization
Research Abstract

The sensitivity of human papilloma virus type 16 (HPV-16) DNA detection by DNA in situ hybridization using biotinylated probes (bio-DISH) was estimated by performing this technique on snapfrozen and peraffin embodied tissue of 10 oral squamous cell carcinomas containing increasing amounts of HPV-16 as determined by Southern blot hybridization. We examined the presence of human papillomavirus (HPV) genome in oral squamous cell carcinoma by employing the polymerase chain reaction. we could not detected HPV16DNA by these method in these tissue. But in situhybridization of this section, HPV16 DNA potitive stained carcinoma around cells were present morphologicaly in 2/10 cases.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

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