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Bleomycin-induced gene expression in E.coli carrying blmA gene

Research Project

Project/Area Number 05454570
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biological pharmacy
Research InstitutionHIROSIMA UNIVERSITY

Principal Investigator

SUGIYAMA Masanori  School of Medicine, Professor, 医学部, 教授 (30106801)

Co-Investigator(Kenkyū-buntansha) INOUYE Yoshio  School of Medicine, Associate Professor, 医学部, 助教授 (00136053)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1994: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1993: ¥5,500,000 (Direct Cost: ¥5,500,000)
KeywordsBleomycim / Inducer / beta-lactamase / Antitumor agent / インデューサー
Research Abstract

A bleomycin (Bm)-producing microorganism, Streptomyces verticillus ATCC15003 possesses two Bm resistance genes designated blmA and blmB.When one of these genes, designatedblmA,was inserted into pUC18 (or pUC19) plasmid and introduced into Escherichia coli TG1, the transformed cells expressed high level-resistance to Bm. In the present study we show that E.coli harboring the blmA-encoding pUC vector overproduced a 30-kDa protein when cultured in the presence of Bm. The Bm-induced protein was identified as the beta-lactamase encoded by the ampicillin resistance gene on the plasmid. In addition, we found that although the Bm family such as pepleomycin, phleomycin, cleomycin and liblomycin gave rise to beta-lactamase induction, bleomycininc acid which lacks terminal amine moiety in Bm molecule, did scarcely. We propose a hypothesis that Bm, an antibiotic inhibitor of DNA synthesis, acts as a an inducer (or an activator) for the expression of a specific gene in the presence of blmA.On the other hand, we constructed a chimeric plasmid designatd as intelligent vector pMAB70, which carried blmA and a tyrosinase structual gene put under the control of beta-lactamase promoter. E.coli harboring pMAB70 produced the melanine pigment, when cultured in the presence of Bm.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (9 results)

All Other

All Publications (9 results)

  • [Publications] Sugiyama,M.: "Characterization by molecular cloning of two genes from Streptomyces verticillus encoding resistance to bleomycin21GC01:Gene" 151. 11-16 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sugiyama,M.: "Inactivation of bleomycin by an N‐acetyltransferase in the bleomycin‐producing strain Streptomyces verticillus" FEMS Microbiology Letters. 121. 81-86 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sugiyama,M.: "Reconbinant Microbes for Industrial and Agricultural Applications" Marcel Dekker,Inc.(Murooka,Y.and Imanaka,T.eds), 875 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sugiyama, M., Thompson, C.J., Kumagai, T., Suzuki, K., Deblaere, R., Villarroel, N.and Davies, J.: "Characterisation by molecular cloning of two genes from Streptomyces verticillus encoding resitance to blemycin" Gene. 151. 11-16 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sugiyama, M., Kumagai, T., Shinoya, M., Kimura, E.and Davies, J.E.: "Inactivation of bleomycin by an Nacetyltransferase in the bleomycin-producing strain Streptopmyces verticillus" FEMS Microbiol.Lett.121. 81-86 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sugiyama, M.: In Recombinant Microbes for industrial and agricultural applications. (Y.Murooka and T.Imanaka eds.) Antibiotics produced by Streptomyces host-vector systems. Marcel Dekker, Inc.(USA), 105-117 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sugiyama M.: "Characterization by molecular cloning of two genes from Streptomyces verticillus encoding resistance to bleomycin" Gene. 151. 11-16 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sugiyama,M.: "Inactivation of bleomycin by an N-acetyltransferase in the bleomycin-producing strain Streptomyces verticillus" FEMS Microbiology Letters. 121. 81-86 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sugiyama,M.: "Recombinant Microbes for Industrial and Agricultural Applications" Marcel Dekker Inc.(Murooka Y.and Imanaka,T.eds), 875 (1994)

    • Related Report
      1994 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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