• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Genetical Improvement of Microbial Ability to Degrade Armatic Pollutants and Its Application to Celanup of Polluted Environments

Research Project

Project/Area Number 05454616
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 環境保全
Research InstitutionOkayama University of Science

Principal Investigator

KIYOHARA Hohzoh  Fac.Engineering, Okayama University of Science, Professor, 工学部, 教授 (50068904)

Co-Investigator(Kenkyū-buntansha) HATTA Takashi  Research Inst.Technol., Okayama University of Science, Asistant Professor, 技術科学研究所, 講師 (00218497)
TAKIZAWA Noboru  Fac.Engineering, Okayama University of Science, Associate Professor, 工学部, 助教授 (50179579)
YAMANKA Kei  Fac.Engineering, Okayama University of Science, Professor, 工学部, 教授 (30035951)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1995: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1994: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1993: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsBioremediation / Polycyclic aromatic hydrocarbons / Polychlorinated aromatics / Pseudomonas putida / Pseudomonas aeruginosa / Rhodococcus / Gene analysis / Dechlorinase / Pseudomoans pickettii
Research Abstract

For application of genetically engineered bacteria to cleanup of polluted environments, bacterial genes involved in the degradation of aromatic hydrocarbons (PAHs) and chlorinated armatics were cloned and analyzed. The following results were obtained ; (1) In Pseudomonas putida OUS82 and P.aeruginosa PaK1, all genes encoding enzymes metabolizing naphthalene to salycylate were highly similar to those i the nah operon of plasmid NAH7. Induction of the ability of OUS82 to degrade PAHs was regulated natively, but that of PaK1 was constitutive. While, genes for naphthalene dihydrodiol dehydrogenase in Rhodococcus sp. CIR2 and 3,4-dihydroxyphenanthrene dioxygenase in Alcaligenes faecalis AFK2 were different from the corresponding genes of NAH7. (2) Two enzymes, two components of the dechlorinase and the ring-cleaving dioxygenase, involved in the degradation of 2,4,6-trichlorophenol in P.pickettie DTP0602 were isolated and characterized from the E.coli cells carrying cloned genes, and the fist structures of the proteins were made clear. (3) In the metabolisms of biphenyl, bebzene, toluene, and ethylbenzene in Rhodococcus sp.RHA1, a single dioxygenase was involved in the ring cleavage of thesefour armatics and two different hydrolases mediated the hydrolysis of the ring-fission products.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Kiyohara,H. et al.: "Cloning and characterization of a chromosomal gene cluster,pah,that encodes the upper pathway for phenanthrene and nhaphtnalene utilization by Pseudomonas putida OUS82" J. Bacteriology. 177. 2439-2443 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takizawa,N. et al.: "Identification and characterization of genes encoding polycyclic aromatic hydrocarbon dioxygenase and polycyclic aromatic hydrocarbon dihydrodiol dehydrogenas in Pseudomonas putia OUS82" J. Bacteriology. 177. 2444-2449 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 浄原法蔵: "微生物による有機科学汚染物質の浄化" 農林水産技術研究ジャーナル. 18,No.3. 23-29 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takizawa,N. et al.: "Alocus of Pseudomonas pickettii DTP0602,had,that encodes 2,4,6-trichloro-4-dechlorinase with hydroxylase activity,and hydroxylation of various chlorophenols by the enzyme" J.Ferment.Bioeng. 80. 318-326 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seto,M. et al.: "Multiple PCB teansformation system in Gram-positivebacterium,Rhodococcus sp.RHAI" Appl. Environment. Microbiol.61. 4510-4513 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seto,M. et al.: "A novel transformation of biphenyls by Rhodococcus sp. strain RHAl" Appl. Environment. Microbiol.61. 3353-3358 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masai,E. et al.: "Characterization of biphenyl catabolic genesof Gram-positive polychlorinated biphenyl degrader Rhodococcus sp.strain RHA1" Appl. Environment. Microbiol.61. 2079-2085 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 浄原法蔵: "多環性芳香族を分解する細菌の素顔、in地球を守る生き物たち一環境微生物とバイオレメディエーション" 技報堂出版(編集:児玉徹、大竹久夫、矢木修身), 238(33-40) (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kiyohara, H.et al.: "Cloning and chracterization of a chromosomal gene cluster, pah, that encodes the upper pathway for phenanthrene and naphthalene utilizaton by Pseudomonas putida OUS82" J.Bacteriology. 177. 2439-2444 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takizawa, N.et al.: "Identificaiton and characterization of genes encoding polycyclic aromatic hydrocarbon dioxygenase and polycyclic aromatic hydrocarbon dihydrodiol dehydrogenas in Pseudomonas putida OUS82" J.Bacteriology. 177. 2444-2449 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Takizawa, N.et al.: "A locus of Pseudomonas pickettii DTP0602, had, that enocdes 2,4,6-trichloro-4-dechlorinase with hydroxylase activity, and hydroxylation of various chlorophenols by the eyzyme" J.Ferment.Bioeng.80. 318-326 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seto, M.et al.: "Multiple PCB teansformation system in Gram-positivebacterium, Rhodococcus sp.RHA1" Appl.Environment.Microbiol.61. 4510-4513 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Seto, M.et al.: "A novel trasformation of biphenyls by Rhodococcus sp.strain RHA1" Appl.Environment.Microbiol.61. 3353-3358 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Masai, E.et al.: "Characterization of biphenyl catabolic genesof Gram-positive polychlorinated biphenyl degader Rhodococcus sp.strain RHA1" Appl.Environment.Mcirobiol.61. 2079-2085 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kiyohara, H. et al.: "Cloning and characterization of a chromosomal gene cluster, pah, that encodes the upper pathway for phenanthrene and naphthalene utilization by Pseudomonas putida OUS82" J. Bacteriology. 177. 2439-2443 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takizawa, N. et al.: "Identification and characteization of genes encoding polycyclic aromatic hydrocarbon dioxygenase and polycyclic aromatic hydrocarbon dihydrodiol dehydrogenas in Pseudomonas putida OUS82" J. Bacteriology. 177. 2444-2449 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 浄原法蔵: "微生物による有機化学汚染物質の浄化" 農林水産技術研究ジャーナル. 18,No.3. 23-29 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Takizawa, N. et al.: "A locus of Pseudomonas pickettii DTP0602, had, that encodes 2,4,6-trichloro-4-dechlorinase with hydroxylase activity, and hydroxylation of various chlorophenols by the enzyme" J. Ferment. Bioeng. 80. 318-326 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Seto, M. et al.: "Multiple PCB teansformation system in Gram-positivebacterium, Rhodococcus sp. RHA1" Appl. Environment. Microbiol.61. 4510-4513 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Seto, M. et al.: "A novel transformation of biphenyls by Rhodococcus sp. strain RHA1" Appl. Environment. Microbiol.61. 3353-3358 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Masai, E. et al.: "Characterization of biphenyl catabolic genesof Gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1" Appl. Environment. Microbiol.61. 2079-2085 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 浄原法蔵: "多環性芳香族を分解する細菌の素顔、in 地球を守る生き物たち-環境微生物とバイオレメディエーション" 技報堂出版(編集:児玉 徹、大竹久夫、矢木修身), 238(33-40) (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kiyohara,H. et al: "Cloning and characterization ofa chromosomal gene cluster,pah,that encodes the upper pathway for phenanthrene and naphthalene utilization by Pseudomonas putida OUS82" Journal of Bacteriology. 177. 2439-2443 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takizawa,N. et al.: "Identification and characterization of genes encoding poiycyclic aromatic hydrocarbon dioxygenase and polycyclic aromatic hydrocarbon dihydrodiol dehydrogenas in Pseudomonas putida OUS82" Journal of Bacteriology. 177. 2444-2449 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 浄原法蔵: "微生物による有機化学汚染物質の浄化" 農林水産技術研究ジャーナル. 18,No.3. 23-29 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Kiyohara et al.: "Cloning and Characterization of a Chromosomal Gene Cluster,pah,That Encodes the Upper Pathway for Phenanthrene and Naphthalene Utilization by Pseudomona putida OUS82" Journal of Bacteriology. 176,(in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] N.Takizawa et al.: "Idenfication and Characterization of Genes Encoding Polycyclic Aromatic Hydrocarbon Dixygenase,pahA,and Polycyclic Aromatic Hydrocarbon Dihydrodiol Dehydrogenase,pahB,in Pseudomonas putida OUS82" Journal of Bacteriology. 176,(in press).

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi