| Project/Area Number |
05454617
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
HIRATUSKA Toshiaki Asahikawa Medical College, Chemistry Associate Professor, 医学部, 助教授 (30041825)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1994: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1993: ¥5,200,000 (Direct Cost: ¥5,200,000)
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| Keywords | Muscle Contraction / Myosin Head / Flexible Regions / Energy Transduction |
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| Research Abstract |
The fluorescent labeling reagent 4-fluoro-7-sulfamoylbenzofurazan (ABDF) specifically labeled Cys-707 (SH1) of myosin subfragment-1 (S-1) . The hydrophobic fluorescent dye 3- [4- (3-phenyl-2-pyrazolin-1-y1) benzene-1-sulfonylamido] phenylboronic acid (PPBA) atoichiometrically bound to the ATP-binding pocket of S-1. These two fluorophores were useful as sensitive probes to examine the flexible regions of S-1. The data suggested a movement of SH1 associated with ATP hydrolysis. Furthermore, the experiments with PPBA clearly demonstrated that the ATP-binding pocket of S-1 assumes a tightened conformational state during ATP hydrolysis. Thus these two flexible regions are responsible for the energy transduction system in muscle contraction.
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