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Cloning of genes encoding putative taste receptor proteins

Research Project

Project/Area Number 05454627
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionHOKKAIDO UNlVERSITY

Principal Investigator

KURIHARA Kenzo  Hokkaido Univ., Fac.Professor, 大学院・薬学研究科, 教授 (00016114)

Co-Investigator(Kenkyū-buntansha) SHOUJI Takayuki  Hokkaido Univ., Fac.of Pharmaceut.Sci.Instructor, 大学院・薬学研究科, 助教授 (00241349)
KASHIWAYANAGI Makoto  Hokkaido Univ., Fac.of Pharmaceut.Sci.Associate Professor, 大学院・薬学研究科, 助教授 (20169436)
MATSUOKA Ichiro  Hokkaido Univ., Fac.of Pharmaceut.Sci.Associate Professor, 大学院・薬学研究科, 助教授 (40157269)
MIYAKE Michihisa  Hokkaido Univ., Fac.of Pharmaceut.Sci.Associate Professor, 大学院・薬学研究科, 助教授 (30133771)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥7,000,000 (Direct Cost: ¥7,000,000)
Fiscal Year 1994: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywordsguanlyly cyclase / G-protein coupled receptor / taste / cloning / putative taste receptor / rat / cattle / olfactory receptor / 味覚受容体 / GTP結合蛋白質 / cDNA / ゼブラフィッシュ / アミノ酸 / リポソーム / 苦味物質 / 甘味物質 / ウシ味覚器 / ペプチド受容体 / cAMP / IP3
Research Abstract

Using reverse transcription-polymerase chain reaction and degenerate oligonucleotide primers, we amplified novel members of two different subfamily of G-protein coupled receptor (GCR) superfamily from bovine taste tissue. Type A receptor clones composed of multiple cDNA clones had significant similarity with putative olfactory receptor subfamily, while a single type B clone had significant similarity with peptide receptor subfamily. Type A clones were expressed in renal as well as in taste tissue.
A number of studies have shown that cGMP may play some roles in taste transduction. For example, guanylyl cyclase is reported to be localized at microvilli of taste cells. To identify the structure of guanylyl cyclase in taste tissue, cDNA fragments encoding guanylyl cyclase catalytic domain wre amplified from rat and bovine tongue epithelium using degenerate oligonuclotide primers and reverse transcription-polymerase chain reaction. Three novel clones, two membrane type guanylyl cyclases (RAT GC-1, BOV GC-3) and one soluble type guanylyl cyclase (RAT GC-2). RAT GC-1 was distributed over various rat tissues in addition to taste tissue. BOV GC-3 was similar to but distinct from recent cloned olfactory specific guanlylyl cyclase. Rat GC-2 was identified as rat homologue of alpha2 subunit of the soluble guanylyl cyclase.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report

Research Products

(28 results)

All Other

All Publications (28 results)

  • [Publications] I.Matsuoka: "Cloning of G-protein-coupled receptors from bovine taste tissue" Olfaction and Taste. XI. 68-72 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Ugawa: "Enhancing effects of salts on canine taste responses to amino acids and umami substances" Olfaction and Taste. XI. 361-364 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Nakamura: "Taste receptor proteins directly extracted by liposome from intact epithelium of bullfrog tongue" Biochem.Biophys.Res.Commun.201. 415-422 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Ugawa: "Enhancement of canine taste responses to umami substances by salts" Am.J.Physiol.26. R944-R949 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 松岡 一郎: "嗅覚・味覚のシグナル伝達機構と受容体" 生化学. 60. 150-154 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 柏柳 誠: "味細胞および嗅細胞における化学物質の認識・識別機構" 油化学. 43. 896-905 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] I.Matsuoka: "Identification of novel guanylyl cyclases from chemosensory tissues of rat and cattle" Biochem.Biophy.Res.Commun.216. 242-248 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 柏柳 誠: "嗅覚・味覚系におけるGTP結合蛋白質共役型受容体" 実験医学. 1.14. 180-182 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 松岡 一郎: "G蛋白質共役型受容体スーパーファミリー" 生体の科学. 46. 545-549 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] I.Matsuoka, T.Mori, J.Aoki and T.Sato: "Cloning of G-protein-coupled receptors from bovine taste tissue, Olfaction and Taste" XI. 68-72 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Ugawa and K.Kurihara: "Enhancing effects of salts on canine taste responses to amino acids and umami substances, Olfaction and Taste" XI. 361-364 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Nakamura, K.Tsujii, Y.Katsuragi, K Kurihara and J.Sunamoto: Biochem.Biophys.Res.Commun.201. 415-422 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Ugawa and K.Kurihara: "Enhancement of canine taste responses to umami substances by salts" Am.J.Physiol.26. R944-R949 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] I.Matsuoka: "Signal transduction mechanism and receptors in olfactory and gustatory organs" Seikagaku. 66. 150-154 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M., Kashiwayanagi and K.Kurihara: "Mechanism of discrimination of chemical stimuli in gustatory and olfactory cells" Yukagaku. 43. 896-905 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] I.Matsuoka, T.Mori, T.Sato, M.Sakai and K.Kurihara: "Identification of novel guanylyl cyclases from chemosensory tissues of rat and cattle" Biochem.Biophy.Res.Commun.216. 242-248 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Kashiwayanagi, I.Matsuoka and K.Kurihara: "G-protein coupled receptors in olfactory and gustatory systems" Jikkenigaku. 1.14. 180-182 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] I.Matsuoka, M.Kashiwayanagi and K.Kurihara: "G-protein coupled receptor superfamily" seitainokagaku. 46. 545-549 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Kashiwayanagi: "A large contribution of a cyclic AMP-independent pathway to turtle olfactory transduction" Journal of General Physiology. 103. 957-974 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Ugawa: "Enhancement of canine taste responses to umami substances by salts" American Journal of Physiology. 266. 944-949 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Kashiwayanagi: "Odor discrimination in single turtle olfactory receptor neuron" Neuroscience Letter. 170. 233-236 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Nakamura: "Taste receptor proteins directly extracted by liposome from intact epithelium of bullfrog tongue" Biochemical Biophysical Research Commurication. 201. 415-422 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Kashiwayanagi: "Discrimination of odorants in the non-olfactory system:analysis of responses of the frog gustatory system to odorants by multidimensional scaling" Comparative Biochemistry and Physiology. 108A. 479-484 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Tohru Ugawa and Kenzo Kurihara: "Large enhancement of canine taste responses to amino acids by salts." Am.J.Physiol.264. R1071-R1076 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ichiro Matsuoka,Tetsuya Mori,Junko Aoki,Taiji Sato and Kenzo Kurihara: "Idetification of novel members of G-protein coupled receptor superfamily expressed in bovine taste tissue." Biochem.Biophys.Res.Comm.194. 504-511 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Yoshihisa Katsuragi and Kenzo Kurihara: "Specific inhibitor for bitter taste." Nature. 365. 213-214 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ichiro Matsuoka,Tetsuya Mori,Junko Aoki and Taiji Sato: "Olfaction and Taste XI,ed.by K.Kurihara,N.Suzuki and H.Ogawa" Springer, 700 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Tohru Ugawa and Kenzo Kurihara: "Olfaction and Taste XI,ed.by K.Kurihara,N.Suzuki and H.Ogawa" Springer, 700 (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-03-31   Modified: 2016-04-21  

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