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Mechanism of Energy Coupling between Catalysis (ATP Synthesis and Hydrolysis) and H^+ Transport in ATP Synthase

Research Project

Project/Area Number 05454630
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionOsaka University

Principal Investigator

FUTAI Masamitsu  Osaka Univ., ISIR-SANKEN,Professor, 産業科学研究所, 教授 (50012646)

Co-Investigator(Kenkyū-buntansha) OKA Toshihiko  Osaka Univ., ISIR-SANKEN,Research Associate, 産業科学研究所, 助手 (40263321)
IWAMOTO Atsuko  Osaka Univ.ISIR-SANKEN,Research Assistant, 産業科学研究所, 教務職員 (70252715)
MORIYAMA Yoshinori  Osaka Univ., ISIR-SANKEN,Research Associate, 産業科学研究所, 助手 (10150658)
田村 茂彦  大阪大学, 産業科学研究所, 助手 (90236753)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1994: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1993: ¥4,200,000 (Direct Cost: ¥4,200,000)
KeywordsATP / ATP Synthase / ATP Synthesis / ATP hydrolysis / ATPase / H^+輸送 / 酵素 / 解媒中心 / 共役機構
Research Abstract

The ATP synthase (FoFl, H^+ATPase) catalyzes ATP synthesis of hydrolysis coupling with proton translocation. Escherichia coli enzyme is similar to those found in inner mitochondrial or chloroplast thylakoid membranes, and has contributed greatly to the understanding of this complicated enzyme. The ctalytic site of the enzyme is in the beta subunit or at the interface between the alpha and beta subunits of the membrane extrinsic Fl sector. The proton pathway is fomed from the a b, and c subunit of the membrane intrinsic Fo sector. The gamma, delta, and epsilon subunits of the Fl are required funcionally and structrually to connect the catalyic subunits to the Fo sector. The mechanism of ATP hydrolysis can be studied using purified (Fl-ATPase).
In this project we defined, by mutational analysis of the E.Coli enzyme, the catalytic site in the beta subunit and the active role (s) of the gamma subunit in the energy coupling between the chemical (ATP synthesis/hydrolysis) and osmotic reaction (proton translocation).
Results obtained for the beta subunit catalytic site were : (1) betaLys-155 and betaThr-156 in the glycine-rich sequence (Cly-Cly-Ala-Cly-Val-Cly-Lys-Thr, residues 149-156) are essential for catalysis ;
(2) betaClu 181 and betaArg-182 in the conserved GER sequence are essential catalytic residues ;
(3) betaCly-149 is close to betaCly-172, betaSer-174, betaClu-192 and betaVal-198. From these results, amodel of the catalytic site is proposed that is consistent with the inhibitor sensitvities of the mutant enzmes. This model is consistent with that obtained from recent X ray structure of Fl. Genetic studies suggested that the gamma subunit plays a role in regulation energy coupling (coupling between catalysis and proton transport) : gammaMet-23, gammaArg-242, and the region between gammaCln-269 and gammaVal-280 are close to each other and interract for efficient energy coupling.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (53 results)

All Other

All Publications (53 results)

  • [Publications] A.Yamamoto.Y.Moriyama,Y.Tashiro,M.Futai: "Inhibition of intracellular transport of newly synthesiged prolactim of bafilomycin A,in a pituitary tumor cell bire,CH3 cells" Eur.J.Cell.Biol.62. 127-139 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Moriyama,T.Manabe,Y.Tashiro and M.Futai: "ATP-dependent uptake anti-neoplastic agents by acidic organelles" J.Biochem.115. 213-218 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Omote,M.-Y,Park,M.Maeda and M.Futai: "The α/β subunit intcraction in H^+-ATPasc(ATP synthasc):an Escherichia coil α subunil mutation(αArg-296→Cys)rcstorcd coupling cllicicncy to the dclctcrious β subunit mutant(βScr-174→Phc)" J.Biol.Chem.269. 10265-10269 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Mushiake,Y.Etani,M.Hasebe,M.Futai,M.Maeda: "Genes of memberes of the GATA-binding protein family (GATA-GT1 and GATA-GT2)together with H^+K^+-ATPase are specifically transcribed in gastric parietal cells" FEBS Lett.340. 117-120 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Futai,A.Iwamoto,H.Omote and M.Maeda: "Catalysis and energy coupling of H^+-ATPase(ATP Synthase):Molecular biolgical approaches" Biochimica et Biophysica Acta. 1187. 165-170 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.-Y,Park,H.Omote,M.Maeda,M.Futai: "Conscrvcd Glu-181 and Arg-182 residucs of Escherichia coil H^+-ATPasc(ATP synthase)β sudunit are csscntial for catalysis:Propcrtics of 33 mutants botwccn βGlu-161 and βLys-201 rcsiducs" J.Biochem.116. 1139-1145 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Iwamoto,Y,Orita,M.Maeda and M.Futai: "N-Ethylmalemide-sensitive mutant(βVal-153→Cys)Escherichia coil F1 ATPase:Cross-Linking of the mutant β subunit with the α subunit." FEBS Lett.352. 243-246 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Uyama,Y.Moriyama,M.Futai and H.Michibata: "Immunological detection of a vacular-type H^+-ATPase in vanadocytes of the ascidian ascidia sydneiensis samea." J.of Experi.Zool.207. 148-154 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yoshimori,Y,Moriyama,M.Futai,Y,Tashiro: "Acidification of phagosomes and degradation of rod outer segments in rat retinal pigment epithelium" Invest,Ophthalmol.Vis.Sci. 35. 568-579 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Moriyama,V.Patel and M.Futai: "Quinnacrine mustard and lipophilic entions inhibitory to both vacuolar H^+-ATPase and FoF1-ATP synthase" Biochem.Biophyis.Res.Common. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S,Ueno,M.Maeda,M.Futai F.Izumi and M.Kawamura: "Functional consequances of the substitution of disulfidc-bondcd scgmcnt,Cys127-Cys150,locatcd in the extraccllular domain of the Na,K-ATPase β subunit:Arg148 is essential for the functional expression of Na,K-ATPase." in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Maeda,T,Nishi,T,Oka,T,Masaya,M.Futai: "The rat intrinsic factor gene:Its 5′-upstream region and chief cell-specific transcription." in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y,Moriyama,A,Yamamoto,M.Maeda,M.Futai: "Microvesicles Isolated from Bovine Posterior Pituitary Accumulate Norepinephrine" in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Ito.M.Imai.Y.Moriyama and M.Futai: "Solution structure of FoF_1-ATPase.Utilisation des neutrons et du rayonnement synchrotron en biologie:diffusion centrale,duffusion duffuse et diffusion inelastique." St Nizier du Moucherotte,France, (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Futai,A.Iwamoto and M.Maeda: "Molecular Aspects of Enzyme Catalysis(H^+-ATPase:catalysis and H^+ translocation)" ‘VCH Publishers,N.Y., (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] R.K.Nakamoto and M.Futai: "The FoF_1 ATP synthase:structure involved in catalysis,transport and coupling." Biomembranes V,(in press),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yosimori, A.Yamamoto, Y.Moriyama, Y.Tashiro, M.Futai: "Inhibition of intracellular transport of newly synthesiged prolactim of bafilonycin A,in a pituiary tumor cell bire, CH3 cells" Eur.J.Cell.Biol. 62. 127-139 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Moriyama, T.Manabe, Y.Tashiro and M.Futai: "ATP-dependent uptake anti-neoplastic agents by acidic organelles" J.Biochem. 115. 213-218 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] H.Omote, M.-Y.Park. M.Maeda and M.Futai: "The alpha/beta subunit intcraction in H^+-ATPasc (ATP synthasc) : an Escherichia coil alpha subunit muation (alphaArg-296*Cys) restored coupling eficiency to the delcterious beta saubunit mutant (betaSer-174*Phc)" J.Biol.Chem. 269. 10265-10269 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Mushiake, Y.Etani, M.Hasebe, M.Futai, M.Maeda: "Genes for members of the GATA-binding protein family (GATA-GT1 and GATA-GT2) together with H^+K^+-ATPase are specifically transcribed in gastric parietal cells" FEBS Lett. 340. 117-120 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Futai, A.Iwamoto, H.Omote and M.Maeda: "Catalysis and energy coupling of H^+-ATPase (ATP Syntase) : Molecular biological approaches" Biochimica et Biophysica-Acta. 1187. 165-170 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M-Y.Park, H.Omote, M.Maeda, M.Futai: "Conserved Clu-181 and Arg-182 rcsiducs of Escherichia coli H^+-ATPasc (ATP synhasc) beta stubunit arc esscntial for catalysis : Propctics of 33 mutants betwccn betaClu-161 and betaLys-201 residucs" J.Biochem. 116. 1139-1145 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Uyama, Y.Moriyama, M.Futai and H.Michibata: "Immunological detection of a vacuolar-type H^+-ATPase in vanadocyteds of the ascidian ascidia sydneiensis samea" J.of Experi.Zool. 207. 148-154 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yoshimori, Y.Moriyama, M.Futai, Y.Tashiro: "Acidification of phagosomes and degradation of rod outer segments in rat retinal pigment epithelium" Invest.Ophthalmol.Vis.Sci. 35. 568-579 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Moriyama, V.Patel and M.Futai: "Quinnacrine mustard and lipophilic entions inhibitory to both vacuolar H^+-ATPase and FoFl-ATP synhase" Biochem.Biophyis.Res.Common. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Ueno, M.Maeda, M.Futai, F.Izumi and M.Kawamura: "Functional consequanccs of the substiution of disulidc-bondcd scgent, Cys127-Cys150, locatcd in the extraccllular domain of the Na, K-ATPase beta subunit : Arg 148 is csscnital for the funcional expression of Na, K-ATPasc" in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Maeda, T.Nishi, S.Mushiake, T.Oka, T.Masaya, M.Futai: "The rat intrnsic factor gene : Its 5'-upsteam region and chief cell-specific transcripition" in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Moriyama, A.Yamamoto, K.Tomochika, M.Maeda, M.Futai: "Microvesicles Isolated form Bovine posterior Pituitary Accumulate Norepinephrine" in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] A.Iwamoto, Y.Orita, M.Maeda, and M.Futai: "N-Ethylmaleimide-sensitive mutant (betaVal-153*Cys) Escherichia coli Fl ATPase : Cross-Linking of the mutant beta subunit with the alpha subunit" FEBS Lett. 352. 243-246 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Ito, M.Imai, Y.Moriyama and M.Futai: "Solution structure of FoFl ATPase, Utilisation des neutrone et du rayonnement synchrotron en biologie : diffusion centale, duffusion duffuse et diffusion inelatique" St Nizier du Moucherotte, France. Nov.21-25 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Futai, A,Iwamoto and M.Maeda: "H^+-ATPase : catalysis and H^+transolcation Molecular Aspects of Enzyme Catalysis (Ed.by T.Fukui and K.Soda)" 211-228/VCH. Publishers. (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] R.K.Nakamoto and M.Futai: "The FoFl ATP synthase : structure involved in catalysis, transport and coupling" Biomembranes V. in press.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Uyama,Y.Moriyama,M.Futai and H.Michibata: "Immunological detection of a vacuolar‐type H^+ATPase in vanadocytes of the ascidian ascidia sydneiensis samea." J.of Experi.Zool.207. 148-154 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Yoshimori,Y.Moriyama,M.Futai,Y.Tashiro: "Acidification of phagosomes and degradation of rod outer segments in rat retinal pigment epithelium" Invest.Ophthalmol.Vis.Sci.35. 568-579 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Moriyama,V.Patel and M.Futai: "Quinnacrine mustard and lipophilic entions inhibitory to both vacuolar H^+ATPase and FoFl‐ATP synthase" Biochem.Biophyis.Res.Common. (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Ueno,M.Maeda,M.Futai,F.Izumi and M.Kawamura.: "Functional conscquanccs of the substitution of disultidc‐bondcd scgmcnt,Cys127‐Cys150,locatcd in the extraeellular domain of the Na,K‐ATPase β subunit:Atg148 is csscntial for the functional expression of Na,K‐ATPasc." (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Maeda,T.Nishi,S.Mushiake,T.Oka,T.Masaya,M.Futai.: "The rat intrinsic factor gene:Its 5′‐upstream region and chief sell‐specific transcription." (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Moriyama,A.Yamamoto,K.Tomochika,M.Maeda,M.Futai: "Microvesicles Isolated from Bovine Posterior Pituitary Accumulate Norepinephrine" (in press).

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Yoshimori,A.Yamamoto,Y.Moriyama,Y.Tashiro,M.Futai: "Inhibition of intracellular transport of newly synthesiged prolactim of bafilomycin A,in a pituitary tumor cell bire,CH3 cells" Eur.J.Cell.Biol.62. 127-139 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Moriyama,T.Manabe,Y.Tashiro and M.Futai: "ATP‐dependent uptake anti‐neoplastic agents by acidic organelles." J.Biochem.115. 213-218 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Omote,M.‐Y.Park,M.Maeda and M.Futai: "The α/β subunit interaction in H^+‐ATPase(ATP synthase):an Escherichia coil α subunit mutation(αArg‐296→Cys)restored coupling efficiency to the delcterious β subunit mutant(βSer‐174→Phc)" J.Biol.Chem.269. 10265-10269 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Mushiake,Y.Etani,M.Hasebe,M.Futai,M.Maeda.: "Genes for members of the GATA‐binding protein family (GATA‐GT1 and GATA‐GT2)together with H^+K^+‐ATPase are specifically transcribed in gastric parietal cells" FEBS Lett.340. 117-120 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Futai,A.Iwamoto,H.Omote and M.Maeda: "Catalysis and energy coupling of H^+‐ATPase(ATP Synthase):Molecular biological approaches" Biochimica et Biophysica Acta. 1187. 165-170 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M‐Y.Park,H.Omote,M.Maeda,M.Futai: "Conserved Glu‐181 and Arg‐182 residucs of Escherichia coli H^+‐ATPase(ATP synthase)β subunit are essential for catalysis:Properties of 33 mutants between β Glu‐161 and β Lys‐201 residues" J.Biochem.116. 1139-1145 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Ito.M.Imai,Y.Moriyama and M.Futai: "St Nizier du Moucherotte,France" Solution structure of FoF_1‐ATPase.Utilisation des neutrons et du rayonnement synchrotron en biologie:diffusion centrale,duffusion duffuse et diffusion inelastique., (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Futai,A.Iwamoto and M.Maeda.: "Molecular Aspects of Enzyme Catalysis(Ed.by T.Fukui and K.Soda)" H^+‐ATPase:catalysis and H^+ translocation, (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] R.K.Nakamoto and M.Futai: "Biomembranes V" The F_0F_1 ATP synthase:structure involved in catalysis,transport and coupling., (in press)

    • Related Report
      1994 Annual Research Report
  • [Publications] ロバート 中本 他2名: "The γ Subunit of the Escherichia coli ATP synthase:mutations in the carboxyl-terminal region restore energy coupling to the amino-terminal mutant γ Met-23→Lys" The Journal of Biological Chemistry.268. 867-872 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 岩本 昌子 他3名: "Domains near ATP phosphate in the catalytic site of H^+-ATPase:model proposed from mutagenesis and inhibitor studies" The Journal of Biological Chemistry.268. 3156-3160 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 田村 茂彦 他3名: "Gastric DNA binding proteins recognize upstream sequence motifs of parietal cell-specific genes" Proc.Natl.Acad.Sci.U.S.A.90. 10876-10880 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 二井 將光 他6名: "The atp2 operon of the green bacterium Chlorobium limicola" Biochim.Biophhys.Acta.1172. 267-273 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 城ノ内 昌宜 他2名: "The α subunit of ATP synthase (F_0F_1):The Lys-175 and Thr-176 residues in the conserved sequence (Gly-X-X-X-X-Gly-Lys-Thr/Ser) are located in the domain required for stable subunit-subunit interaction" J.Biochem. 114. 171-176 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 表 弘志 他3名: "The α/β subunit interaction in H^+-ATPase (ATP synthase):an Escherichia coil α subunit mutation (αArg-296→Cys) restored coupling efficiency to the deleterious β subunit mutant (βSer-174→Phe)" The Journal of Biological Chemistry. (in press). (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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