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MOLECULAR MECHANISM OF HYPERPOLARIZATION OF ON-BIPOLAR CELLS IN RETINA

Research Project

Project/Area Number 05454634
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionHIMEJI INSTITUTE OF TECHNOLOGY

Principal Investigator

TSUDA Motoyuki  HIMEJI INSTITUTE OF TECHNOLOGY,FACULTY OF SCIENCE,PROFESSOR, 理学部, 教授 (60045458)

Co-Investigator(Kenkyū-buntansha) NIKKAWA Satoshi  HIMEJI INSTITUTE OF TECHNOLOGY,FACULTY OF SCIENCE,ASSISTANT PROFESSOR, 理学部, 助手 (90244681)
NAKAGAWA Masashi  HIMEJI INSTITUTE OF TECHNOLOGY,FACULTY OF SCIENCE,ASSISTANT PROFESSOR, 理学部, 助手 (00212085)
IWASA Tatsuo  HIMEJI INSTITUTE OF TECHNOLOGY,FACUTLY OF SCIENCE,ASSOCIATE PROFESSOR, 理学部, 助教授 (00133926)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,500,000 (Direct Cost: ¥6,500,000)
Fiscal Year 1994: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥4,600,000 (Direct Cost: ¥4,600,000)
KeywordsRETINA / GULTAMATE RECEPTOR / SECOND NEURON / HYPERPOLARIZAION / BIPOLAR CELL / G-PROTEIN / SYNAPSE / RECEPTOR
Research Abstract

Light reduce the release of glutamate from photoreceptor cell, resulting in opening of cation channels on on-bipolar cell and subsequent depolarization of the cell. In this respect, retinal onbipolar cell is a novel neuron which hyperpolarizes on contact with neurotransmitter molecules. Electrophysiological studies showed that cGMP act as the second messenger mediating the action of glutamate through the G protein that striking similarities to transduction in photoreceptors. In order to elucidate molecular mechanism of hyperpolarization of on-bipolar cell, we studied signal coupling proteins of on-bipolar cell in bovine retina.
GTP-binding protein (G protein) linking to metabotropic glutamate receptor of bovine retinal on-bipolar cell was studied by use of pharmacologically selective ligands, 2-amino-4-phosphonobutyric acid (APB) on bacteria toxin-catalyzed ADP-ribosylation and GTP S-binding. In contrast to the electrophysiological finding reported, G-protein coupling to APB-sensitive glutamate receptor served as a substrate for pertussis toxin but did not for cholera toxin. Several glutamate analogues effective on on-bipolar cell, as well as APB,increased GTP S binding to retinal membranes devoid of rod outer segments. The enhancement of GTP S binding by APB was completely abolished when the membranes were pretreated with pertussis toxin and NAD.These results suggest that, in retinal on-bipolar cell, the G-protein which couples metabotropic glutamate receptor to hyperpolarizing response of the cell is sensitive to pertussis toxin.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] S.Masuda,et al.: "Infrared Studies of Octopus Rhodopsin:Existence of a Long‐lived Intermediate and the States of the Carboxylic Group of Asp‐81 in Rhodopsin" FEBS Lett.317. 223-227 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Masuda,et al.: "Infrared Studies of Octopus Rhodopsin and Lumi‐rhodopsin," J.Mol.Struct.297. 29-34 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yoshioka,et al.: "Biophysical Aspect of Information Flow from Receptor to Channel," NY Acad.Sci.Annals. 707. 328-337 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kikkawa,et al.: "GTP‐binding proteins couples with metabotropic glutamate receptor in bovine retinal on‐bipolar cell.," Biochem.Biophys.Res.Commun.195. 374-379 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kikkawa,et al.: "GTP‐binding proteins coupling to glutamate receptors on bovine retinal membranes.," NY Acad.Sci.Annals. 707. 557-560 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Liang,et al.: "The pKa of the Protonated Schiff base of Gecko Cone and Octopus Visual Pigments." Biophys.J.,. 67:2. 848-854 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Tsuda,: "Regulation of Signal Coupling Proteins in Octopus Photoreceptors.“Frontiers of Photobiology".,(A.Shima et al.Ed.)" Elsevier Science Publishers., 568 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 津田基之: "ロドプシン、「レセプター-基礎と臨床」" 朝倉書店、, 944 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Masuda, E.H.Morita, M.Tasumi, T.Iwasa and M.Tsuda: "Infrared Studies of Octopus Rhodopsin : Existence of a Longlived Intermediate and the States of the Carboxylic Group of Asp81 in Rhodopsin and its Photoproducts." FEBS Lett.317. 223-227 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Masuda, E.H.Morita, M.TasumiT.Iwasa, M.Tsuda: "Infrared Studies of Octopus Rhodopsin and Lumi-rhodopsin" J.Mol.Struct. 297. 29-34 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] T.Yoshioka, H.Inoue, K.Ichikawa, M.Sakakibara, M.Tsuda, H.Suzuki: "Biophysical Aspect of Information Flow from Receptor to Channel" NY Acad.Sci.Annals. 707. 328-337 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kikkawa, M.Nakagawa, T.Iwasa, and M.Tsuda: "GTP-binding proteins couples with metabotropic glutamate receptor in bovine retinal on-bipolar cell." Biochem.Biophys.Res.Commun.195. 374-379 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Kikkawa, M.Nakagawa, T.Iwasa, and M.Tsuda: "GTP-binding proteins coupling to glutamate receptors on bovine retinal membranes." NY Acad.Sci.Annals. 707. 557-560 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Liang, J., Steinberg, G., Livnah, N., Sheves, M., Ebrey, T., Tsuda, M.(1994): "The pKa of the Protonated Schiff base of Gecko Cone and Octopus Visual Pigments." Biophys.J.67 : 2. 848-854

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Tsuda, T.Iwasa, M.Nakagawa, S.Kikkawa and T.Tsuda: "Regulation of Signal Coupling Proteins in Octopus Photoreceptors." "Frontiers of Photobiology". , Elsevier Science Publishers.189-194 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Masuda,et al.: "Infrared Studies of Octopus Rhodopsin:Existence of a Long-lived Intermediate and the States of the Carboxylic Group of Asp-81 in Rhodopsin" FEBS Lett.317. 223-227 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Masuda,et al.: "Infrared Studies of Octopus Rhodopsin and Lumi-rhodopsin," J.Mol.Struct.297. 29-34 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Yoshioka,et al.: "Biophysical Aspect of Information Flow from Receptor to Channel," NY Acad.Sci.Annals. 707. 328-337 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Kikkawa,et al.: "GTP-binding proteins couples with metabotropic glutamate receptor in bovine retinal on-bipolar cell.," Biochem.Biophys.Res.Commun.195. 374-379 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Kikkawa,et al.: "GTP-binding proteins coupling to glutamate receptors on bovine retinal membranes.," NY Acad.Sci.Annals. 707. 557-560 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] Liang,et al.: "The pKa of the Protonated Schiff base of Gecko Cone and Octopus Visual Pigments." Biophys.J.,. 67:2. 848-854 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Tsuda,: "Regulation of Signal Coupling Proteins in Octopus Photoreceptors.“Frontiers of Photobiology".,(A.Shima et al.Ed.)" Elsevier Science Publishers., 568 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 津田基之: "ロドプシン、「レセプター-基礎と臨床」" 朝倉書店、, 944 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Masuda,et al: "Infrared Studies of Octopus Rhodopsin" FEBS Lett.317,. 223-227 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Masuda,et al: "Infrared Studies of Octopus Rhodopsin and Lumi‐rhodopsin," J.Mol.Struct.297,. 29-34 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Kikkawa,et al: "GTP‐binding Proteins Couples with Metabotropic Glutamate Receptor in Bovine Retinal On‐bipolar Cell.." Biochem.Biophys.Res.Commum.195,. 347-379 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Tsuda,et al: "Frontiers of Photobiology″." Elsevier Science Publishers., 568 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 津田 基之: "レセプター-基礎と臨床" 朝倉書店, 944 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2019-02-28  

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