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S-modulin-like proteins in the retina.

Research Project

Project/Area Number 05454635
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionKeio UniversitySchool of Medicine

Principal Investigator

KAWAMURA Satoru  Keio University School of Medicine, Department of Physiology, Associate Professor, 医学部, 助教授 (80138122)

Co-Investigator(Kenkyū-buntansha) MIWA Naohumi  Keio University School of Medicine, Department of Physiology, Sssistant, 医学部, 助手 (40255427)
KUWATA Osamu  Keio University School of Medicine, Department of Physiology, Assistant, 医学部, 助手 (30255426)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥4,300,000 (Direct Cost: ¥4,300,000)
Fiscal Year 1994: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsS-Modulin / Rhodopsin / Visinin / Photoreceptor / Adaptation / Calcium / Recoverin / Phosphorylation / カルシウム結合蛋白質 / 網膜
Research Abstract

In the frog retina, we previously purified an S-modulin-like protein, s26. In the present project, we examined (1) the aminoacid sequence, (2) the tissue localization and (3) the function of s26. In addition, the possible function of S-modulin-like proteins found in other tissues are studied.
(1) The amino acid sequence of s26 is 66% identical to S-modulin. Surprisingly, s26 shows 77% identity, a higher homology to visinin, a cone specific calcium-biding protein in chick retina.
(2) The antibody raised to a peptide specific to s26 stained a photoreceptor layr of the frog retina.The exact location of the positive signal in the layr is now in progress.
(3) The function of s26 seems to be the same as that of S-modulin. This conclusion was drawn from the finding that s26 inhibited rhodopsin phosphorylation at high calcium concentrations as S-modulin does.
(4) Many S-modulin-like proteins have been found in brain, but their functions are titally unknown. We examined 7 of these proteins and found that all of the 7 proteins tested showed a similar effect as S-modulin.This finding strongly suggests that these proteins function as a calciumdependent regulator of a phoshorylation reaction.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (48 results)

All Other

All Publications (48 results)

  • [Publications] Kawamura,S.: "Rhodopsin phosphorylation as a mechanism of cGMP phosphodiesterase regulation by S-modulin." Nature. 362. 855-857 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura,S.: "Recoverin has S-modulin activity in frog rods." J.Biol.Chem.268. 14579-14582 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 河村 悟: "視細胞における明順応の分子機構" 科学. 63. 303-311 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura,S.: "Molecular aspects of photoreceptor adaptation in vertebrate retina." Int.Rev.Neurobio.35. 43-86 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 河村 悟: "S-モジュリン" 生体の科学. 44. 247-248 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 河村 悟: "視細胞の順応とカルシウム結合蛋白質" Clinical Calcium. 3. 44-48 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 河村 悟: "視細胞の順応調節蛋白質S-モジュリン" 医学の歩み. 169. 1110-1111 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 河村 悟: "視細胞機能発現のメカニズム:受容体からチャネルまで、「イオンチャネルとレセプター研究の最近の進歩」" 実験医学. 12. 1424-1429 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura,S.: "Molecular mechanism of light-adaptation in vertebrate photoreceptors." J.Photochem.Photobiol.23. 270-271 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura,S.: "Inhibition of rhodopsin phosphorylation by non-myristoylated recombinant recoverin." Biochem.Biophys.Res.Commun.203. 121-127 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura,S.: "Photoreceptor light-adaptation mediated by S-modulin,a member of a possible regulator family of protein phosphorylation in signal transduction." Neurosci.Res.20. 293-298 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura,S.: "Unsolved issues in S-modulin/recoverin study.(印刷中)" Behav.Brain Res.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura,S.: "S-modulin.In“Guidebook to the calcium-binding Proteins"" Oxford University press(印刷中),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura,S.: "Phototransduction,excitation and adaptation.In“Neurobiology and Clinical Aspects of the Outer Retina"" Chapman & Hall(印刷中),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S.: "Rhodopsin phosphorylation as a mechanism of cGMP phosphodies terase regulation by S-modulin." Nature. 362. 855-857 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S., Hisatomi, O., Kayada, S., Tokunaga, F., and Kuo, C.-H: "Recoverin has S-modulin activity in frog rods." J.Biol.Chem.268. 14579-14582 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S., Cox, J.A., and Nef, P.: "Inhibition of rhodopsin phosphory lation by nonmyr is toy lated recombinant recoverin." Biochem.Biophys.Res.Commun.203. 121-127 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S.: "Molecular aspects of photoreceptor adaptation in vertebrate retina." Internatl.Rev.Neurobiol.35. 43-86 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S.: "Molecular mechanism of Photoreceptor light-adaptation.(in Japanese)" Kagaku. 632. 303-311 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S.: "S-modulin. (in Japanese)" Seitaino-kagaku. 44. 247-248 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S.: "Photoreceptor adaptation and calcium-binding proteins. (in Japanese)" Clinical Calcium. 3. 1476-1480 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S.: "S-modulin, a regulatory protein of photoreceptor adaptation.(in Japanese)" Igakuno-ayumi. 169. 1110-1111 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S.: "Mechanism of photoreceptor function : from a receptor molecule to an ion channel. (in Japanese) , In "Recent Progress in the Study of Ion Channels and Receptors".ed.by Mishina, M.and Mikoshiba, K." Jikken-igaku. 12. 1424-1429 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S.: "Molecular mechanism of light-adaptation in vertebrate photoreceptors." J.Photochem.Photobiol.23. 270-271 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S.: "Photoreceptor light-adaptatoin mediated by S-modulin, a member of a possible regulator family of protein phosphorylation in signal transduction." Neurosci.Res.20. 293-298 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S.: "Phototransduction, excitation and adaptation." in Neurobiology of the vertebrate outer retina.ed.by Aecher, S., Djamgoz, M.and Vallerga, S.Chapman & Hall, London. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S.: "Unsolved issues in S-modulin/recoverin study." Behav.Brain Sci.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S.and Kaneko, A.: Molecular mechanism of vision : retina.(in Japanese)Frontier in molecular neurophysiology. Kouseisha (in press.),

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura, S.: S-modulin.in Guidebook to the calcium binding-Proteins.ed.by Celio, M.R.Oxfoed University Press, (in press.),

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Kawamura,S.: "Inhibition of rhodopsin phosphory lation by non-myristoy lated recombinant recoverin." Biochem.Biophys.Res.Commun.203. 121-127 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 河村 悟: "視細胞の順応調節蛋白質S-モジュリン" 医学の歩み. 169. 1110-1111 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 河村 悟: "視細胞機能発現のメカニズム:受容体からチャネルまで、" 実験医学. 12. 1424-1429 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawamura,S.: "Molecular mechanism of light-adaptation in vertebrate photoreceptors." J.Photochem.Photobiol.23. 270-271 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawamura,S.: "Photoreceptor light-adaptation mediated by S-modulin,a member of a possible regulator family of protein phosphorylation in signal transduction." Neurosci.Res.20. 293-298 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawamura,S.: "Unsolved issues in S-modulin/recoverin study." Behav.Brain Res.(印刷中).

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawamura,S.(分担): "S-modulin.In"Guidebook to the calcium-binding Proteins"" Oxford University Press(印刷中),

    • Related Report
      1994 Annual Research Report
  • [Publications] Kawamura,S.(分担): "Phototransduction,excitation and adaptation.In"Neurobiology and Clinical Aspects of the Outer Retina"" Chapman & Hall(印刷中),

    • Related Report
      1994 Annual Research Report
  • [Publications] Satoru Kawamura: "Rhodopsin phosphorylation as a mechanism of cGMP phosphodiesterase regulation by S-modulin." Nature. 362. 855-857 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Satoru Kawamura: "Recoverin has S-modulin activity in frog rods." Journal of Biological Chemistry22GD02:268. 14579-14582 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Satoru Kawamura: "Molecular aspects of photoreceptor adaptation in vertebrate retina." International Review of Neurobiology. 35. 43-86 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 河村 悟: "視細胞における明順応の分子機構" 科学. 63. 303-311 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 河村 悟: "S-モジュリン" 生体の科学. 44. 247-248 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 河村 悟: "視細胞の順応とカルシウム結合蛋白質" Clinical Calcium. 3. 1476-1480 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Satoru Kawamura: "Molecular mechanism of light-adaptation in vertebrate photoreceptors.21GC07:Journal of Photochemistry and photobiology" (in press).

    • Related Report
      1993 Annual Research Report
  • [Publications] 河村 悟: "視細胞の順応調節蛋白質S-モジュリン" 医学のあゆみ. (印刷中).

    • Related Report
      1993 Annual Research Report
  • [Publications] 河村 悟: "視細胞機構発現のメカニズム:受容体からチャネルまで" 「イオンチャネルとレセプター研究の最近の進歩」実験医学、増刊. (印刷中).

    • Related Report
      1993 Annual Research Report
  • [Publications] 河村 悟: "分子神経科学の最先端 分担執筆 視覚の分子構造-網膜-" 厚生社(印刷中),

    • Related Report
      1993 Annual Research Report
  • [Publications] Satoru Kawamura: Chapman & Hall,London(in press)27HC02:Neurobiology oh the vertebrate outer retina 分担-Phototransduction.excitation and adaptation.-,

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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