• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular Mechanisms of Spontaneous Mutagenesis Caused by Oxygen Radicals

Research Project

Project/Area Number 05454639
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Molecular biology
Research InstitutionNara Institute of Science and Technology (1994)
The University of Tokyo (1993)

Principal Investigator

HISAJI Maki  Nara Institute of Science and Technology, バイオサイエンス研究科, 教授 (20199649)

Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥5,800,000 (Direct Cost: ¥5,800,000)
Fiscal Year 1994: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥3,800,000 (Direct Cost: ¥3,800,000)
KeywordsEsherichia coli / Molecular Biology / Mutator / Mismatch repair / DNA replication
Research Abstract

8-Oxo-dGTP (8-oxo-7,8-dihydrodeoxyguanosine triphosphate) is a potent mutagenic substrate for DNA synthesis, and organisms possess enzymes specifically hydfolyzing 8-oxo-dGTP to the monophosphate form. The accumulatiom of 8-oxo-dGTP in the nucleotide pool induces G : C*T : A transversion as well as A : T*C : G transversion, and Escherichia coli cells possess mechanisms for preventing such mutations. From analyzes of forward mutations induced in cells lacking 8-oxo-dGTPase (MutT protein) and/or repair enzymes that suppress mutations caused by 8-oxoguanine DNA (MutM and MutY proteins), cooperative functions of these proteins in control of the spontaneous mutagenesis became evident. In mutator strains lacking MutT and/or MutM proteins, 8-oxoguanine of DNA increased to a concentration expected from the increased frequency of mutation.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Pascale Belguise-Valladier: "Effect of single DNA lesions on in vitro replication with DNA polymerase III holoenzyme:Comparison with other polymereses" J.Mol.Biol. 236. 151-164 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takayasu Itoh: "Roles of transcription repair in alkylation mutagenesis" Matation Res.314. 273-285 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tatsurou Tajiri: "Functional Cooperation of MutT,MutM and MutY proteins in preventing mutations caused by spontareous oxidation of quanine nucleotide in Escherichia coli." Mutation Res.(1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Pascale Belguise-Valladier, Hisaji Maki, Mutsuo Sekiguchi and Robert P.P.Fuchs: "Effect of single DNA lesions on in vitro replication with DNA polymerase III holoenzyme : Comparison with other polymerases." J.Mol.Biol.236. 151-164 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Takayasu Itoh, Takanori Nakamura, Hisaji Maki and Mutsuo Sekiguchi: "Roles of transcription and repair in alkylation mutagenesis." Mutation Res.314. 273-285 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Tatsurou Tajiri, Hisaji Maki and Mutsuo Sekiguchi: "Functional Cooperation of MutT,MutM and MutY proteins in preveting mutations caused by spontaneous oxidation of guanine nucleotide in Escherichia coli." Mutation Res.(in press, ). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Pascale Belguse-Valladier: "Effect of single DNA lesions on in vitro replication with DNA polymerase III holoenjume:Coucoparison with other polymevases." J.Mol.Biol. 236. 151-164 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Takayasu Itoh: "Roles of trakscription and repair in alkylation mutagenesis." Mutation Res.314. 273-285 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sakumi,K.: "Cloning and expression of cDNA for a human enzyme that hydrolyzes 8-oxo-dGTP,a mutagenic substrate for DNA synthesis." Journal of Biological Chemistry. 268. 23524-23530 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Belguise-Valladier,P.: "Effect of single DNA lesions on in vitro replication with DNA polymerase III holoenzyme." Journal of Molecular Biology. (発表予定). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ito,T.: "Roles of transcription and repair in alkylation mutagenesis." Mutation Research. (発表予定). (1994)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi