| Project/Area Number |
05454641
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
OHSHIMA Yasumi Kyushu Univ., Dept.of Biology, Professor, 理学部, 教授 (90037606)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1994: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1993: ¥4,200,000 (Direct Cost: ¥4,200,000)
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| Keywords | mRNA / nuclear export / fission yeast / in situ hybridization / cosmid library / poly-A / ts mutants / in situ hybridization / コスミドライブライ- / オリゴdT / ディゴキシゲニン |
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| Research Abstract |
Approximately 1,000 temperature-sensitive mutants for growth in the fission yeast S.pombe were screened by in situ hybridization with oligo dTlabeled with digoxigenin. Eleven mutants were found to accumulate poly-A RNA in the nucleus at the restrictive temperature, five of which (AZ60, S22, S2, AK-H1, AK-H6) were better candidates as mRNA export mutants. At least three complementation groups were found for mutations in the five strains. Cosmid clones which complements the temperature-sensitive defects in each of S22 and AK-H6 were isolated and are being analyzed
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