| Project/Area Number |
05454645
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | KYOTO UNIVERSITY |
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Principal Investigator |
UEDA Yukihiko (1994) Kyoto Univ.Dept.Medicine Assistant Professor, 医学部, 助手 (70252434)
井本 敬二 (1993) 京都大学, 医学部, 助教授 (00176512)
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| Co-Investigator(Kenkyū-buntansha) |
HAKAMATA Yasuhiro Kyoto Univ.Dept.Medicine Assistant Professor, 医学部, 助手 (30261233)
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| Project Period (FY) |
1993 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1994: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥4,400,000 (Direct Cost: ¥4,400,000)
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| Keywords | ryanodine receptor / calcium release channel / distribution gene expression / immunosuppresssive agent FK506 / human lymphocyte / カルシウムチャンネル / cDNAクローニング / チャンネルの活性化 |
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| Research Abstract |
The neuronal Ca^<2+>signal is induced by a rise in the intracellular free Ca^<2+> concentration and is thought to be important for higher brain function., To define the contribution of neuronal Ca^<2+> signalling via the ryanodine receptor (RYR) , we examined RYR type specific gene expression in rabbit brain by in situ hybridization histochemistry. Skeletal type RYR was highly expressed in cerebellar Purkinje cells. Cardiac type RYR was predominantly expressed throughout nearly the entire brain. Brain type RYR was high in the hippocampal CA1 pyramidal layr, caudate, putamen and dorsal thalamus.
Cloning and sequence analysis of cDNA showed that the brain type of RYR is expressed in human Jurkat T-lymphocyte cells. Fura-2 measurements revealed that the RYR in T-cells functions as a ryanodine-sensitive, caffeine-insensitive Ca^<2+> release channel. Furthermore, ryanodine stimulated proliferation and altered growth pattern of cultured human T-cells when added together with FK506.
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