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Study on the pathogenesis of cortical delayd neuronal death after transient cerebral ischemia

Research Project

Project/Area Number 05454657
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Nerve anatomy/Neuropathology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KUROIWA Toshihiko  Tokyo Medical and Dental University, MRI,Associate Prof., 難治疾患研究所, 助教授 (80129832)

Co-Investigator(Kenkyū-buntansha) OKEDA Riki  Tokyo Medical and Dental University, MRI,Professor, 難治疾患研究所, 教授 (70013977)
HORIKAWA Junsei  Tokyo Medical and Dental University, MRI,Associate Prof., 難治疾患研究所, 助教授 (50114781)
TANIGUCHI Ikuo  Tokyo Medical and Dental University, MRI,Professor, 難治疾患研究所, 教授 (60014255)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥5,400,000 (Direct Cost: ¥5,400,000)
KeywordsCerebral Cortex / Cerebral Ischemia / Delayd Neuronal Death / Succinate Dehydrogenase / 神経細胞壊死 / エネルギー代謝障害 / ATP / エネルギー代謝不全 / 脳梗塞
Research Abstract

Various mechanisms such as impaired protein synthesis and microcirculatory disturbance have been proposed to explain the mechanism of delayd neuronal death. We examined regional differences in the activity of a mitochondrial respiratory enzyme, succinate dehydrogenase, in normal and postischemic gerbils using the quantitative imaging method.
Gerbils of either sex were subjected to 5 min.of bilateral common carotid artery occlusion or permanent occlusion of left common carotid artery, and sacrificed at various time intervals after the ischemia. Some gerbils were sacrificed after a sham procedure. Coronal sections of the forebrain at 1.5mm posterior to the bregma were prepared for quantitative imaging of succinic dehydrogenase (SDH) activity. Histological change was evaluated using the same section after immersion fixation in buffered formalin and staining with HE and KB.In the control gerbils, SD activity in the pyramidal cell layr of the CA 1 sector was lower than in any other area of the hippocampus. In gerbils after transient ischemia, SD activity remained lowest in the Pyramidal cell layr of the CA 1 sector during the examination period. Histologically, selective neuronal necrosis was observed in the Pyramidal layr of the CA 1 sector at 2d and thereafter. After permanent occlusion of the common carotid artery, SDH activity decreased initially at the head of caudate and middle layr of cortex. The area of reduced SDH activity gradually enlarged to the whole area supplied by the ipsilateral carotid artery. Ischemic change by histological examination corresponded to the area of reduced SDH activity. These results showed that poor reserves of respiratory enzyme is the primary factor determining its selective vulnerability.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (56 results)

All Other

All Publications (56 results)

  • [Publications] Kuroiwa T,: "Regional tissue compliance of edematous brain after cryogenic injury in cats." Intracranial Pressure VIII. 8. 122-129 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,: "Microradiographic examination of edema fluid distribution in the cat brain after cryogenic injury." Intracranial Pressure VIII. 8. 130-134 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 谷口郁雄、: "光学的計測による脳梗塞急性期の神経活動の画像化" 病態生理. 12. 801-807 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,Okeda R.: "Neuropathology of cerebral ischemia and advances in experimental studies on its pathogenesis." Pathology International. 44. 171-181 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,: "Is the swelling in brain edema isotropic or anisotropic?" Acta Neurochir 〔Suppl〕. 60. 155-157 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,: "Biomechanical Characteristics of brain edema : the difference between vasogenic-type and cytotoxic-type edema." Acta Neurochir 〔Suppl〕. 60. 158-161 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,: "Swelling of the white matter is anisotropic in vasogenic brain edema." J Cereb Blood Flow Metabol. 13. 91 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 黒岩俊彦: "虚血脳に於けるコハク酸脱水素酵素定量的画像化." 神経病理学. 13. 67 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 黒岩俊彦、: "浮腫脳のコンプライアンスの変化." Jap J Cereb Blood Flow Metabol. 5. 110 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 上木雅人、: "脳虚血発症後早期のエネルギー代謝障害は斑状に出現する" Jap J Cereb Blood Flow Metabol. 5. 134 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,: "Recruitment of penumbra tissue into infarction process in experimental cerebral ischemia." Folia Neuropathologica. 32. 181 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 黒岩俊彦: "実験的脳虚血に於けるエネルギー代謝障害進展の様式" 神経病理学. 14. 316 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ijima T,: "Blood-brain barrier opening following transient reflex sympathetic hypertension." Acta Neurochir 〔Suppl〕. 60. 142-144 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Czernicki Z,: "Effect of acetazolamide on early ischemic cerebral edema in gerbils." Acta Neurochir 〔Suppl〕. 60. 329-331 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,: "Regional tissue compliance of edematous brain in water intoxication edema-Comparison to vasogenic edema." Intracranial Pressure IX. 9. 175-178 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Piao Y,: "Prevention by Minaprine of ischemic neuronal damage and memory impairment in Mongolian gerbils." Jpn J Psychopharmacol. 15. 157-163 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,: "Quantitative imaging of succinic dehydrogenase activity in gerbil brain after unilateral common carotid artery occlusion." J Cereb Blood Flow Metabo L. 13. 738 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,: "Swelling of the white matter is anisotropic in vasogenic brain edema." J Cereb Blood Flow Metabol. 13. 91 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 黒岩俊彦: "虚血脳に於けるコハク酸脱水素酵素定量的画像化." 神経病理学. 13. 67 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 黒岩俊彦、: "浮腫脳のコンプライアンスの変化." Jap J Cereb Blood Flow Metabol. 5. 110 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 上木雅人、: "脳虚血発症後早期のエネルギー代謝障害は斑状に出現する" Jap J Cereb Blood Flow Metabol. 5. 134 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,: "Recruitment of penumbra tissue into infarction process in experimental cerebral ischemia." Folia Neuropathologica. 32. 181 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 黒岩俊彦、: "実験的脳虚血に於けるエネルギー代謝障害進展の様式" 神経病理学. 14. 316 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 黒岩俊彦: "脳梗塞病巣辺縁部ISCHEMIC PENUMBRAにおけるエネルギー代謝障害の経時的な進展様式の検討" 難治疾患研究所年報. 22. 193-194 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 黒岩俊彦: "一過性全脳虚血後の海馬錐体神経細胞の選択的脆弱性と細胞内呼吸酵素活性の相関" 難治疾患研究所年報. 23(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T, Terakado et al.: "The pyramidal cell layer of sector CA 1 shows the lowest hippocampal succinate dehydrogenase activity in normal and postischemic gerbils" Neuroscience letters. 206. 1-4 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ito U,Baethmann A,Hossmann KA,Kuroiwa T,et al: "Brain Edema IX.I" Springer-Verlag, 589 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,Taniguchi I and Okada R: "Regional tissue compliance of edematous brain after cryogenic injury in cats" Intracranial Pressure VIII Springer-Verlag. 127-129 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,Yokofujita J,Kaneko H,Okeda R: "Microradiographic examination of edema fluid distribution in the cat brain after cryogenic injury" Intracranial Pressure VIII Springer-Verlag. 130-134 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,Okeda R: "Neuropathology of cerebral ischemia and advances in experimental studies on its pathogenesis" Pathology International. 44. 171-181 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,Ueki M,et al: "Is the swelling in brain edema iso tropic or anisotropic?" Acta Neurochir [Suppl] (Brain Edema IX). 60. 155-157 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,Ueki M,Chen O,Suemasu H,Taniguchi, I,Okeda R: "Biomechanical Characteristics of brain edema : the difference between vasogenic-type and cytotoxic-type edema" Acta Neurochir [Suppl] (Brain Edema IX). 60. 158-161 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ito U,Baethmann A,Hossmann KA,Kuroiwa T,Marmarou A,Reulen H (Editors): Brain Edema IX.Springer-Verlag. 1-589 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ijima T,Kubota Y,Kuroiwa T,Sankawa H: "Blood-brain barrier opening following transient reflex sympathetic hypertension" Acta Neurochir [Suppl] (Brain Edema IX). 60. 142-144 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Czernicki Z,Kuroiwa T,Ohno K,Endo S,Ito U: "Effect of acetazolamide on early ischemic cerebral edema in gerbils" Acta Neurochir [Suppl] (Brain Edema IX). 60. 329-331 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,Ueki M,Taniguchi I,Ichiki H,Okeda R: "Regional tissue compliance of edematous brain in water intoxication edema-Comparison to vasogenic edema" Intracranial Pressure IX.Springer-Verlag. 175-178 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Piao Y,Mineshita S,Kuroiwa T,Koike Y: "Prevention by Minaprine of ischemic meuronal damage and memory impairment in Mongolian gerbils" Jpn J Psychopharmacol. 15. 157-163 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,Terakado M,Yamaguchi T,Endo S,Ueki M,Okeda R: "The pyramidal cell layr of sector CA 1 shows the lowest hippocampal succinate dehydrogenase activity in normal and postischemic gerbils" Neuroscience letters. 206. 1-4 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,Czernicki Z,Ueki M,Kaneko H,Okeda R: "Quantitative imaging of succinic dehydrogenase activity in gerbil brain after unilateral common carotid artery occlusion" J Cereb Blood Flow Metabol. 13. S738 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroiwa T,Ueki M,Okeda R: "Recruitment of penumbra tissue into infarction process in experimental cerebral ischemia" Folia Neuropathologica. 32 (3). 181 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Piao Y,Kuroiwa T,他: "Prevention by Minaprine of ischemic neuronal damage and memory impairment in Mongolian gerbils." Jpn J Psychopharmacology. 15. 157-163 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 黒岩俊彦他: "大脳海馬Sommer's sectorにおけるコハク酸脱水素酵素活性の定量的画像化と遅発性神経細胞壊死" 日本病理学会会誌. 84. 273- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 黒岩俊彦他: "スナネズミ全脳虚血の虚血時間による反応性高血流および遅発性低血流の出現時間・強度の変化" Jap J Cereb Blood Flow Metabol. 7. 158- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 黒岩俊彦他: "一過性全脳虚血後の海馬錐体神経細胞の選択的脆弱性と細胞内呼吸酵素活性の相関" 神経病理学. 15. 68- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kuroiwa T: "Review article : Neuropathology of cerebral ischemia and hypoxia : Recent advances in experimental studies on its pathogenesis." Pathology International. 44. 171-181 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kuroiwa T: "Is the swelling in brain edema isotropic or anisotropic?" Acta Neurochir [Suppl] (Brain Edema IX). 60. 155-157 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kuroiwa T: "Biomechanical Characteristics of brain edema : the difference between vasogenic-type and cytotoxic-type edema." Acta Neurochir [Suppl] (Brain Edema IX). 60. 158-161 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Ijima T: "Blood-brain barrier opning following transient reflex sympathetic hypertension." Acta Neurochir [Suppl] (Brain Edema IX). 60. 142-144 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Czernicki Z: "Effect of acetazolamide on early ischemic cerebral edema in gerbils." Acta Neurochir [Suppl] (Brain Edema IX). 60. 329-331 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kuroiwa T: "Regional tissue compliance of edematous brain in water intoxication edema" Intracranial Pressure. TX. 175-178 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kuroiwa T,: "Regional tissue compliance of edematous brain after cryogenic injury in cats." Intracranial Pressure VII Springer-Verlag. 8. pp127-129 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kuroiwa T,: "Microradiographic examination of edema fluid distribution in the cat brain after cryogenic injury." Intracranial Pressure VII Springer-Verlag. 8. pp130-134 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kuroiwa T,: "Quantitative imaging of succinic dehydrogenase activity in gerbil brain after unilateral common carotid artery occlusion." J Cereb Blood Flow Metabol. 13:. S738 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Kuroiwa T,: "Swelling of the white matter is anisotropic in vasogenic brain edema." J Cereb Blood Flow Metabol. 13:. S91 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 黒岩俊彦: "脳虚血病巣に於けるエネルギー代謝障害の定量的画像化法の開発" 難治疾患研究所年報. 21巻. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 黒岩俊彦: "血管原性脳浮腫液の脳内分布に関する研究" 難治疾患研究所年報. 21巻. (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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