| Project/Area Number |
05454657
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KUROIWA Toshihiko Tokyo Medical and Dental University, MRI,Associate Prof., 難治疾患研究所, 助教授 (80129832)
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| Co-Investigator(Kenkyū-buntansha) |
OKEDA Riki Tokyo Medical and Dental University, MRI,Professor, 難治疾患研究所, 教授 (70013977)
HORIKAWA Junsei Tokyo Medical and Dental University, MRI,Associate Prof., 難治疾患研究所, 助教授 (50114781)
TANIGUCHI Ikuo Tokyo Medical and Dental University, MRI,Professor, 難治疾患研究所, 教授 (60014255)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,800,000 (Direct Cost: ¥6,800,000)
Fiscal Year 1995: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1994: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1993: ¥5,400,000 (Direct Cost: ¥5,400,000)
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| Keywords | Cerebral Cortex / Cerebral Ischemia / Delayd Neuronal Death / Succinate Dehydrogenase / 神経細胞壊死 / エネルギー代謝障害 / ATP / エネルギー代謝不全 / 脳梗塞 |
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| Research Abstract |
Various mechanisms such as impaired protein synthesis and microcirculatory disturbance have been proposed to explain the mechanism of delayd neuronal death. We examined regional differences in the activity of a mitochondrial respiratory enzyme, succinate dehydrogenase, in normal and postischemic gerbils using the quantitative imaging method.
Gerbils of either sex were subjected to 5 min.of bilateral common carotid artery occlusion or permanent occlusion of left common carotid artery, and sacrificed at various time intervals after the ischemia. Some gerbils were sacrificed after a sham procedure. Coronal sections of the forebrain at 1.5mm posterior to the bregma were prepared for quantitative imaging of succinic dehydrogenase (SDH) activity. Histological change was evaluated using the same section after immersion fixation in buffered formalin and staining with HE and KB.In the control gerbils, SD activity in the pyramidal cell layr of the CA 1 sector was lower than in any other area of the hippocampus. In gerbils after transient ischemia, SD activity remained lowest in the Pyramidal cell layr of the CA 1 sector during the examination period. Histologically, selective neuronal necrosis was observed in the Pyramidal layr of the CA 1 sector at 2d and thereafter. After permanent occlusion of the common carotid artery, SDH activity decreased initially at the head of caudate and middle layr of cortex. The area of reduced SDH activity gradually enlarged to the whole area supplied by the ipsilateral carotid artery. Ischemic change by histological examination corresponded to the area of reduced SDH activity. These results showed that poor reserves of respiratory enzyme is the primary factor determining its selective vulnerability.
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