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Molecular and Cellular Biological Study on the Actions of Intracellular Calcium Ion in Cultured Cells

Research Project

Project/Area Number 05454668
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Neurochemistry/Neuropharmacology
Research InstitutionKumamoto University

Principal Investigator

MIYAMOTO Eishichi  Kumamoto University, Pharmacology, Professor, 医学部, 教授 (50109659)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Hideyuki  Kumamoto University, Pharmacology, Assistant Professor, 医学部, 講師 (60191433)
FUKUNAGA Kohji  Kumamoto University, Pharmacology, Associate Professor, 医学部, 助教授 (90136721)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥6,600,000 (Direct Cost: ¥6,600,000)
Fiscal Year 1994: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1993: ¥4,700,000 (Direct Cost: ¥4,700,000)
KeywordsCalcium mobilization / CaM kinase II / Neuron / Glia / Enzyme activation / Glutamate receptor / Long-term potentiation / Culturesd cell / プロテインホスファターゼ / 蛋白質燐酸化反応
Research Abstract

Nerve impulses, various neurotransmitters, and growth hormones exert their effects through elevation of the intracellular concentration of calcium ion (Ca^<2+>). Ca^<2+> is involved in several types of neuronal functions including biosynthesis of neurotransmitters, stimulus-secretion coupling of neurotransmitters and hormones, microtubule assembly-diassembly cycle, synaptic plasticity, gene expression and many metabolic reactions. These include the short-term and long-term events with respect to time course. Although the precise molecular mechanisms mediating the actions of Ca^<2+> in the brain diverse, accumulating evidence suggests the effects of Ca^<2+> are mediated through Ca^<2+>-binding proteins. Furthermore, many of the functions are ultimately conducted by protein kinases and protein phosphatases in a Ca^<2+>-dependent manner. In an attempt to elucidate the functions in the central nervous system through calcium signaling pathways, we have been examining the dynamic state of activation of Ca^<2+>/calmodulin-dependent protein kinase II (CaM kinase II) in primary cultures of neurons and glias and established cell systems. We found that CaM kinase II is activated through elevation of intracellular Ca^<2+> in response to the extracellular stimuli. Firthermore, with concomitant activation of the enzyme, phosphorylation of endogenous substrates, activation of the enzyme and long-term potentiation were observed.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (36 results)

All Other

All Publications (36 results)

  • [Publications] F.Arakane: "Glycolipids isolated from Aplysia kurodai can activate cyclic adenosine 3',5'-monophosphate-dependent protein kinase from rat brain." J.Neurochem.62. 86-93 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Yano: "Activation of Ca^<2+>/calmodulin-dependent protein kinase II and phosphorylation of intermediate filament proteins by stimulation of glutamate receptors in cultured rat cortical astrocytes." J.Biol.Chem.269. 5428-5439 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] N.Todutomi: "Brakykinin-evoked non-specific cationic current in neuroblastoma-glioma hybrid (NG108-15)cells and its down-regulation through differentiation." Brain Res.657. 202-206 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] N.Yanagihara: "Occurrence and activation of Ca^<2+>/calmodulin-dependent protein kinase II and its endogenous substrates in bovine adrenal medullary cells." Mol.Pharmacol.46. 423-430 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Goto: "Cellular localization of type II calcium/calmodulin-dependent protein kinase in the rat basal ganglia and intrastriatal grafts derived from the fetal striatal primordia,in comparison with that of Ca^<2+>/calmodulin-regulated protein phosphatase,calcineurin." Neuroscience. 62. 695-705 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Tsutsui: "Correlation of activation of Ca^<2+>/calmodulin-dependent protein kinase II with catecholamine secretion and tyrosine hydroxylase activation incultuted bovine adrenal medullary cells." Mol.Pharmacol.46. 1041-1047 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Fujimoto, H.Yasue, K.Nakao, H.Yamamoto, Y.Hitoshi, M.Jougasaki, K.Okumura, H.Ogawa, K.Takatsu and E.Miyamoto: "Novel monoclonal antibodies specific for human cardiac myosin lightchain l : Useful tools for analysis of normal and pathological hearts" J.Histochem.Cytochem.41. 35-42 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Fukunaga, T.Kobayashi, S.Tamura and E.Miyamoto: "Dephosphorylation of autophosphorylated Ca^<2+>/calmodulin-dependent prtein kinase II by protein phosphatase 2C" J.Biol.Chem.268. 133-137 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] K.Fukunaga, L.Stoppini, E.Miyamoto and Muller: "Long-term potentiation is associated with an increased activity of Ca^<2+>/calmodulin-dependent protein kinase II" J.Biol.Chem.268. 7863-7867 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Hisanaga, S.Yasugawa, T.Yamakawa, E.Miyamoto, M.Ikebe, M.Uchiyama and T.Kishimoto: "Dephosphorylation of microtubule-binding sites at the neuro-filaments-H tail domain by alkaline, acid, and protein phosphatases" J.Biochem. 113. 705-709 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Nagano, H.Yamamoto, K.Harada, E.Miyamoto and M.Futatsuka: "Comparativestudy of modification and degradation of neurofilament proteins in rats subchronically treated with allyl chloride, acrylamide of 2,5^hexanedione" Environmental Res.63. 229-240 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Goto, S.Nagahiro, K.Korematsu, Y.Ushio, E.Miyamoto and W.Hofer: "Cellularcolocalization of calcium/calmodulin-dependent protein kinase II and calcineurin in the rat cerebral cortex and hippocampus" Neurosci.Lett.149. 189-192 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Ohgushi, K.Kugiyama, K.Fukunaga, T.Murohara, S.Sugiyama, E.Miyamoto andH.Yasue: "Protein kinase C inhibitors prevent impairment of endothelium-dependent relaxation by oxidatively modified LDL" Arteriosclerosis and Thrombosis. 13. 1525-1532 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.Arakane, K.Fukunaga, S.Araki, S.Abe, M.Satake, K.Miyazaki, H.Okamura andE.Miyamoto: "Glycolipids isolated from Aplysia kurodai can activate cyclic adenosine 3', 5'-monophosphate-dependent protein kinase from rat brain" J.Neurochem.62. 86-93 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Ysno, K.Fukunaga, Y.Ushio and E.Miyamoto: "Activation of Ca^<2+>/calmodulin-dependent protein kinase II and phosphorylation of intermediate filament proteins by stimulation of glutamate receptors in cultured rat cortical astrocytes" J.Biol.Chem.269. 5428-5439 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] W.-J.Wang, K.Fukunaga, S.Goto and E.Miyamoto: "Purification of Ca^<2+>/calmodulin-dependent protein kinase IIfrom rat stomach and its localization in the mucosa" Biomedical Res.15. 231-240 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Goto, K.Yamada, Y.Ushio, K.Fukunaga and E.Miyamoto: "Neostriatal mosaic and type II calcium/calmodulin-dependent protein kinase : an immunohistochemical study on the adult rat striatum" Neurosci.Lett.173. 177-180 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Y.Oishi, H.Yamamoto and E.Miyamoto: "Changes in fiber-type composition and myosin heavy-chain IId isoform is rat soleus muscle during recovery period after hindlimb suspension" Eur.J.Appl.Physiol.68. 102-106 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] N.Tokutomi, Y.Tokutomi, K.Fukunaga, E.Miyamoto and K.Nishi: "Bradykinin-evoked non-specific cationic current in neuroblastoma-glioma hybrid (NG108-15) cellsand its down-regulation through differentiation" Brain Res.657. 202-206 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] N.Yanagihara, Y.Toyohira, H.Yamamoto, Y.Ohta, M.Tsutsui, E.Miyamoto and F.Izumi: "Occurrence and activation of Ca^<2+>/calmodulin-dependent protein kinase II and its endogenous substrates in bovine adrenal medullary cells" Mol.Pharmacol.46. 423-430 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] S.Goto, K.Yamada, T.Oyama, K.Korematsu, S.Nagahiro, Y.Ushio, K.Fukunaga, E.Miyamoto and W.Hofer: "Cellular localization of type II calcium/calmodulin-dependent protein kinase in the rat basal ganglia and intrastriatal grafts derived from the fetal striatal primordia, in comparison with that of Ca^<2+>/calmodulin-regulated protein phosphatase, calcineurin" Neuroscience. 62. 695-705 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] M.Tsutsui, N.Yanagihara, E.Miyamoto, A.Kuroiwa and F.Izumi: "Correlation of activation of Ca^<2+>/calmodulin-dependent protein kinase II with catecholamine secretion and tyrosine hydroxylase activation cultured bovine adrenal medullary cells" Mol.Pharmacol.46. 1041-1047 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] F.Arakane: "Glycolipids isolated from Aplysia kurodai can activate cyclic adenosine 3',5'-monophosphate-dependent protein kinase from rat brain." J.Neurochem.62. 86-93 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Yano: "Activation of Ca^<2+>/calmodulin-dependent protein kinase II and phosphorylation of intermediate filament proteins by stimulation of glutamate receptors in cultured rat cortical astrocytes." J.Biol.Chem.269. 5428-5439 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Tokutomi: "Bradykinin-evoked non-specificcationic current in neuroblastoma-glioma hybrid(NG108-15)cells and its down-regulation through differentiation." Brain Res.657. 202-206 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] N.Yanagihara: "Occurrence and activation of Ca^<2+>/calmodulin-dependent protein kinase II and its endogenous substrates in bovine adrenal medullary cells." Mol.Pharmacol.46. 423-430 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Goto: "Cellular localization of type II calcium/calmodulin-dependent protein kinase in the rat basal ganglia and intrastriatal grafts derived from the fetal striatal primordia,in comparison with that of Ca^<2+>/calmodulin-regulated protein phosphatase,calcineurin." Neuroscience. 62. 695-705 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] M.Tsutsui: "Correlation of activation of Ca^<2+>/calmodulin-dependent protein kinase II with catecholamine secretion and tyrosine hydroxylase activation in cultured bovine adrenal medullary cells." Mol.Pharmacol.46. 1041-1047 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Fukunaga: "Dephosphorylation of autophosphorylated Ca^<2+>/calmodulin-dependent protein kinase II by protein phosphatase 2C" J.Biol.Chem.268. 133-137 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Fukunaga: "Long-term protentiation is associated with an increased activity of Ca^<2+>/calmodulin-dependent protein kinase II" J.Biol.Chem.268. 7863-7867 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Hisanaga: "Dephosphorylation of microtubule-biniding sites at the neurofilaments-H tail domain by alkaline,acid,and protein phocphatases" J.Biochem.113. 705-709 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Nagano: "Comparative study of modification and degradation of neurofilament proteins in rats subchronically treated with allyl chloride,acrylamide or 2,5-hexanedione" Env.Res.63. 229-240 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Goto: "Cellular colocalization of calcium/calmodulin-dependent protein kinase II and calcineurin in the rat cerebral cortex and hippocampus" Neurosci.Lett.149. 189-192 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Ohgushi: "Protein kinase Cinhibitors prevent impairment of emdothelium-dependent relaxation by oxidatively modified LDL" Arteriosclerosis and Thrombosis. 13. 1525-1532 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 宮本英七: "生体における情報伝達(田中千賀子、西塚泰美・編集)3.1cyclic nucleotide-dependent protein kinase" 南江堂、東京, 7 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 宮本英七: "レセプター基礎と臨床(井村裕夫、岡哲雄、芳賀達也、岸本英爾・編集)3.5タンパク質リン酸化反応" 朝倉書店、東京, 8 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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