| Project/Area Number |
05454685
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
神経・脳内生理学
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| Research Institution | Tokyo Metropolitan Institute of Gerontology (TMIG) |
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Principal Investigator |
SATO Akio Tokyo Metropoliten Institute of Gerontology Dept.Auton.Nerv.Syst., vice-president, 自律神経部門, 副所長 (60072980)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Atsuko Tokyo Metropoliten Institute of Gerontology Dept.Cetral Nerv.Syst., researcher, 中枢神経部門, 研究員 (60214863)
HOTTA Harumi Tokyo Metropoliten Institute of Gerontology Dept.Auton.Nerv.Syst., researcher, 自律神経部門, 研究員 (70199511)
永井 信夫 (財)東京都老人総合研究所, 自律神経部門, 助手 (90260281)
柏木 仁美 (財)東京都老人総合研究所, 自律神経部門, 助手 (00260282)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥6,400,000 (Direct Cost: ¥6,400,000)
Fiscal Year 1995: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1994: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1993: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | natural kiler cell / splenic sympathetic nerve / splenic blood flow / somatic stimulation |
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| Research Abstract |
The effects of non-noxious and noxious mechanical stimulation of the skin on cytotoxic activity of splenic natural killer (NK) cells and splenic blood flow were examined in anesthetized rats.Bilateral brushing of the body surface between the lateral chest and hindlimb for 30 min did not significantly influence cytotoxic activity of splenic NK cells, splenic blood flow or splenic sympathetic efferent nerve activity.Bilateral pinching stimulation of the skin of the hindpaws for 30 min reduced cytotoxic activity of splenic NK cells and splenic blood flow to 67 <plus-minus> 8 and 82 <plus-minus> 3% of the control values, respectively.Pinching hindpaws for 30 min increased splenic sympathetic efferent nerve activity ton 143 <plus-minus> 15% of control.Electrical stimulation of the splanchnic nerve produced a decrease in the cytotoxic activity of splenic NK cells and splenic blood flow.The hindpaw pinching-induced suppression of the splenic sympathetic nerve or the spinal cord at the cervical level.These results indicate that the supersession of cytotoxic activity of NK cells and splenic blood flow following noxious pinching stimulation of hindpaws is a supra-spinal reflex response mediated via the splenic sympathetic nerve.On the other hand, pinching of abdomen for 30 min reduced cytotoxic activity of splenic NK cells both in the central nervous system intact and spinalized rats.Therefore, the abdominal stimulation suppressed the NK activity via spinal reflex pa
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