DEVELOPMENT OF BIOLOGICAL SPECIMEN PREPARATION TECHNIQUE FOR ATOMIC FORCE MICROSCOPE

• Project/Area Number: 05507002
• Research Category: Grant-in-Aid for Developmental Scientific Research (A)
• Allocation Type: Single-year Grants
• Research Field: Bacteriology (including Mycology)
• Research Institution: KYUSHU UNIVERSITY
• Principal Investigator: AMAKO Kazunobu KYUSHU UNIVERSITY , Fuculty of Medicine, PROFESSOR., 医学部, 教授 (20078752)
• Co-Investigator(Kenkyū-buntansha): FUJITA Koichi NISSEI SANGYO CO., LTD.HEAD, 係長 ; MORIYA Tetsuhiro KYUSHU UNIVERSITY,Fuculty of Medicine, Research Associate., 医学部, 助手 (10140790) ; UMEDA Akiko KYUSHU UNIVERSITY,Fuculty of Medicine, Assistant Professor., 医学部, 講師 (30078604)
• Project Period (FY): 1993 – 1994
• Project Status: Completed (Fiscal Year 1994)
• Budget Amount: ¥34,000,000 (Direct Cost: ¥34,000,000); Fiscal Year 1994: ¥4,000,000 (Direct Cost: ¥4,000,000); Fiscal Year 1993: ¥30,000,000 (Direct Cost: ¥30,000,000)
• Keywords: AFM / STM / PROBE MICROSCOPE / BIOLOGICAL SPECIMENS / PLASMA COATING TECHNIQUE / PHAGE / 細菌ベン毛 / レプリカ法

Research Abstract

The most important problem to be solved in the observation of biological specimens by AFM is to stabilize the specimens aginst the scanning force of the probe of the microscope. Without any specific treatment the biological specimens such as phage particles or the bacterial flagella has disappeared from the image after 1st or 2nd scanning. To stabilize the specimen we tried to use the thin membrane of methane or butane. After coating the specimen with these thin membrane we could have good image even after 5-8 scanning with the probe. The thichness of the membrane can be controlled less than 1nm. In such thin membrane we could see the fine structure of bacterial surface layr, the arrangement of protein subunits of S-layr. Our conclusion is that the coating of the membrane is the useful technique for the observation of biological specimens by AFM.

Research Products

• [Publications] K.Amako: "Imaging of the surface structure of Epon thin sections created with a glass Knife and a diamond Knife by an atomic force microscope" Journal of Electron Microscope. 42. 121-123 (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 天児 和暢: "原子間力顕微鏡による生物の観察" 日本電子顕微鏡学会第59回学術講演会. (1993)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 天児 和暢: "原子間力顕微鏡による細菌の観察" 第63回日本細菌学会総会. (1992)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] K.Amako: "Imaging of the surface structure of epon thin section created by atomic force microscope." J.electron Micscope. 42. 121-123 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K.Amako: "Obervation of biological specimen by AFM." Abstract of Japanese Society of Electron Microcopy. (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K.Amako: "Obervation of bacterial specimen by AFM." Abstract of Japanese Society of bacteriologist.(1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] K.Amako: "Imaging of the surface structure of Epon thin sections created with a glass knife and a diamond knife by an atomic force microscope" Journal of Electron microscopy. 42. 121-123 (1993)
• [Publications] 天児和暢: "原子間力顕微鏡による生物の観察" 日本電子顕微鏡学会第59回学術講演会. (1993)
• [Publications] 天児和暢: "原子間力顕微鏡による細菌の観察" 第63回日本細菌学会総会. (1992)
• [Publications] K.Amako: "Imaging of the surface structure of Epon thin section created with a glass knife and a diamond knife by an atomic force microscope" Journal of Electron Microscope22GD01:42. 121-123 (1993)