| Project/Area Number |
05555116
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
計測・制御工学
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| Research Institution | Toyota Physical and Chemical Research Institute |
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Principal Investigator |
IGARASHI Isemi Toyota Physical and Chemical Research Institute Research Scientest, 研究員 (90072678)
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| Co-Investigator(Kenkyū-buntansha) |
HOTTA Nigishi The Third Department of Internal Medicine, Nagoya University School of Medicine,, 医学部第3内科, 教授 (60023793)
SHICHIRI Motosuke Department of Metabolic Medicine, Kumamoto University Medical School, Professor, 医学部代謝内科, 教授 (00028515)
FUJITA Hiroyuki The University of Tokyo, Institute of Industrial Science, Professor, 生産技術研究所・第3部, 教授 (90134642)
SAITO Masao Tokyo Denki University Dept.Information and Communication Engineering, Professor, 工学部・情報通信工学科, 教授 (60010708)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥9,600,000 (Direct Cost: ¥9,600,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1993: ¥3,800,000 (Direct Cost: ¥3,800,000)
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| Keywords | Micro Gulucose Sensor / Autonomous Active Sensor / Micro Actuator / Medical Applications of Micromachine Technology / Medical Application of Ultrasonic Micro-cleaning System / Platinum Black Sensor / マイクロマシンの医用応用 / グルコースセンサ / 自律センサ / 超音波洗浄 / 生体埋込み型センサ |
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| Research Abstract |
The key point of the development of the implantable artificial pancreas is that of a small implantable glucose sensor which can measure blood glucose levels directly for a long time. We have proposed an in situ ultrasonic cleaning method to remove the adhered materials on the sensor surface from blood or body fluid. We have developed a micro glucose sensor fabricated in a needle using a newly developed electrodeposition method of Pt black with physical stability. Pt black was electrodeposited on a Pt write (0.127 mmphi) sealed in a 23-G needle (ID = 0.45 mmphi) under irradiation of ultrasonic wave (38 kHz, 30 W) from a conventional Pt-electroplating solution for 80 s at -80 mV vs.Ag-AgCl. Pt black tightly adhered on the surface of a Pt wire, while that deposited without the irradiation of ultrasonic wave was easily removed from the surface by sonication. GOD was absorbed to the Pt black surface from 0.75 ml of 5300 U/ml solution (pH 7.45) at 6ﾟC for 30 min and air-dried. GOD was fixed with glutaraldehyde and then with Nafion solution at 6ﾟC.The activity of GOD immobilized on the Pt black was found to be 5.5 mU/sensor on the first day and it gradually decreased with time but kept more than 74% of the initial value after 26 days storing in PBSS at 6ﾟC.The sensor responds to the stepwise change of glucose concentrations up to about 50 mg/dl within a few minutes and the output currents varied with oxygen partial pressures.
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