| Project/Area Number |
05555229
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
工業物理化学
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
MATSUNAGA Tadashi Tokyo University of Agriculture and Technology, Department of Biotechnology, Professor, 工学部, 教授 (10134834)
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Kenji Asahi Denka Kogyo K.K., Research and Development Division, Researcher, 研究企画部, 研究員
CHIBA Tomoyuki Tokyo Medical College Hospital, Associate Professor, 医学部, 講師
NAKAMURA Noriyuki Tokyo University of Agriculture and Technology, Department of Biotechonology, As, 工学部, 講師 (20198229)
SODE Koji Tokyo University of Agriculture and Technology, Department of Biotechnology, Ass, 工学部, 助教授 (10187883)
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| Project Period (FY) |
1993 – 1995
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| Project Status |
Completed (Fiscal Year 1995)
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| Budget Amount *help |
¥19,100,000 (Direct Cost: ¥19,100,000)
Fiscal Year 1995: ¥5,500,000 (Direct Cost: ¥5,500,000)
Fiscal Year 1994: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1993: ¥7,600,000 (Direct Cost: ¥7,600,000)
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| Keywords | allergy / allergen / IgE antibody / blood / chemical mediator / biosensing / 食物アレルギー / 白血球 / サイクリックボルタメトリー / メディエーター |
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| Research Abstract |
Many immunoassay methods have been developed for sensing or detecting allergens. The skin tests including prick test and scratch test are typical methods. However, the these tests are dangerous because of anaphylaxis by serum antibodies. Therefore, a safe, simple and rapid method for detecting allergic reaction is required in the clinical and food industry fields. We have investigated the electrochemical method of detection of foods which cause an allergic reaction. A sample containing protein fraction of food was added to cells and incubated. When the potential was applied in the range 0-1.0V vs.saturated calomel electrode, an anodic peak current appeared at around 0.3V.This peak current, attributed to serotonin, increased with time, and the maximum current was obtained 20-25 min of allergen addition. Then, a relationship between applied potential and current difference of whole blood with and without serotonin was examined using whole blood and interdigitated array electrode. The current difference was maximized around 0.35V.The current increased linearly with the increasing concentration of serotonin in the range of 50 to 800 ng/ml. The response of the RBL-1 cells was specific to protein fraction of wheat.
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