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Characterization and Application of Polysaccharide-coated O/W emulsion

Research Project

Project/Area Number 05555256
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 高分子構造・物性(含繊維)
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SUNAMOTO Junzo  Kyoto University, Graduate School of Engineering Professor, 工学研究科, 教授 (80037811)

Co-Investigator(Kenkyū-buntansha) YAMAGUCHI Shigehiko  Nippon Oil Fat Company, Research Associate, 筑波研究所, 研究員
SATO Tadashi  Nippon Oil Fat Company, director, 筑波研究所, 所長
OKUMURA Yukihisa  Kyoto University, Graduate School of Engineering, Assistant Professor, 工学研究科, 助手 (40243042)
AKIYOSHI Kazunari  Kyoto University, Graduate School of Engineering, Associate Professor, 工学研究科, 助教授 (90201285)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥7,600,000 (Direct Cost: ¥7,600,000)
Fiscal Year 1995: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1994: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1993: ¥5,200,000 (Direct Cost: ¥5,200,000)
KeywordsPolysaccharide / Amphiphilic / pullulan / cholesterol / O / W emulsion / antitumor Agent / alpha-linolenicacid / Drug Carrier / α-リノレン酸 / 多糖 / 両観媒性
Research Abstract

We developed a liposome as cated with a naturally occurring polysaccharide which was partly substituted by cholesterol groups in order to make the liposome mrte physicochemically stable and more cell specific. Furthermore, we reported that O/W-lecithin emulsion is stabilized by coating with the hydrophobized polysaccharide. In this work, we employed a new and improved formulation for a very lipofilic and liquid drug, selectively cytotoxic alpha-linolenic acid (ALA). This methodology involves the colloidal stabilization of a O/W-emulsion by coating the surface of oil droplets of ALA with or without TriC_8 with a hydrophobized polysaccharide such as cholesterol-bearing pullulan, CHP and studied its in vivo antitumor activity.
The O/W-emulsion was prepared by ultrasonication of a mixture of CHP and ALA in the presence or absence of trioctanoylglyceride (TriC_8). Colloidal stability of the CHP/ALA emulsion was largely improved by adding TriC_8. Intraperitoneal injection of the CHP/ALA-emulsion effectively prolonged the survial, especially, of C3H/He mice which received intraperitoneal transplantation of MM46 mammary tumore cells. The growth of the same tumor cells subcutaneously transplanted in C3H/He mice also was significantly suppressed without any loss of body weight when CHP/ALA/TriC_8-emulsion was intravenously injected. By using such the colloidally stable O/W-emulsion, a systemic administration of a very lipophilic and liquid drug became possible.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] K.Akiyoshi: "Self-assembly of Hydrophobiged polysaccharide Structure of Hydrogel Naroparticle and Complexation with Organic Compounds" Proc.Japan Acad.71(B). 15-19 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Z.Guo: "Artificial Cell wall for Plant Protoplast.Coating of Plasma Membrane with Hydrophobiged Polysacharides" Chem.Lett.1995. 415-416 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Akiyoshi: "Stabiligation of Insulin upon Supramoleular Complexation with Hydrophobijed Polysaccharide Naroparticle" Chem.Lett. 1995. 707-708 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 三井佳和: "コレステローループルラン分子集合体と牛血清アルブミンとの相互作用に関する熱的研究" 熱測定. 22. 125-130 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 秋吉一成: "両親媒性多糖のヒドロゲルナノ微粒子形成と蛋白質の包接・安定化" 生物物理. 35. 33-36 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Akiyoshi: "Hydrogel Naroparticle formed by self-assembly of Hydrophobged Polysaccharide Stabilization of Adriamycin upon Complexation" Eur.J.Pharmaceutics and Biopharmaceutics. (in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Akiyoshi: "Sell-Assembly of Hydrophobized Polysaccharide. Structure of Hydrogel Nanoparticle and Complexation with Organic Compounds." Proc.Japan Acad.71 (B). 15-19 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Z.Guo: "Artificial Cell Wall for Plant Protoplast. Coating of Plasma Membrane with Hydrophobized Polysaccharides" Chem.Lett. 415-416 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Akiyoshi: "Stablization of Insulin upon Supramolecular Complexation with Hydrophobized Polysaccharide Nanoparticle" Chem.Lett. 707-708 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Mitsui: "Thermodynamic Characterization of Interaction between Self-Aggregates of Cholesterol-bearing Pullulan and Bovine Serum Albumin" Netsu Sokutei. 22. 125-130 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Akiyoshi: "Chracteistics of hydrogel nanoparticle of amphiphilic polysaccharide ; Complexation with globular proteins and their stabilization" Biophysics. 35. 33-36 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K.Akiyoshi: "Hydrogel nanoparticle formed by self-assembly of hydrophobized polysaccharides. Stabilization of adriamycin upon complexation" Eur.J.of Pharmaceutics and Biopharmaceutics. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] K. Akiyoshi: "Self-assembly of Hydrophobized Polysaccharide. Sfructure of Hydrogel Naroparticle and Complexation with Organic Compounds" Proc. Japan Acad.71(B). 15-19 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Akiyoshi: "Artificial Cell Wall for Plant Protoplast. Coating of Plasma Membrane with Hydrophobized Polysaccharides." Chem. Lett.1995. 415-416 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Akiyoshi: "Stabilization of Insulin upon Supramoleular Complexation with Hydrophobized Polysaccharide Naroparticle" Chem. Lett.1995. 707-708 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 秋吉一成: "コレステローループルラン分子集合体と牛血清アルブミンとの相互作用に関する熱的研究" 熱測定. 22. 125-130 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 秋吉一成: "両親媒性多糖のヒドロゲルナノ微粒子形成と蛋白質の包接・安定化" 生物物理. 35. 33-36 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] K. Akiyoshi: "Hydrogel Naroparticle formed by self-assembly of Hydrophobized Polysaccbaride Stabilization of Adriamycin upon Complexation" Eur. J. Pharmaceutics and Biopharmaceutics. (in press). (1996)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Nishikawa: "Suptamoleular Aoenbly belwren Nenoparticle of Hydrophobized Iulysacclarnde and Soluble Prbins" Macromalecules. 27. 7654-7659 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] E.Kang: "Partclion of Palysaccbaride-coated liposomes in agueous two-prase systems" Int.J.Biol.Macronol. 16. 348-353 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Deguchi: "Solution progerty of hydrophobized pullulan corjugated with poly (echylene oxide)-ply(propylene oxide)-paly (echylene oxide)block copalymer" Macromol.Rapid.Commun.15. 705-711 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Akiyoshi: "Selt-assembly of Hydrophobizul Pelysaccharide.Structure of Hydrogel Naroparicle and Complexation with Orgaric Compounds" Proc,Japan Acad.,Ser.B. 71. in press (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] E.Kato: "Interaclion between Ganglioside-containing Liposome and rol T-Lymphocyte" Biochem.Biophys.Res.Commun.203. 1750-1755 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 福井洋樹: "Anticancer Activity of Polysaccahride-polyunsaturated Fatty Acid Complex" J.Bioactive and Biocompatible polym.8. 305-316 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 秋吉一成: "Self-aggregates of Hydrophobized Polysaccharides in Water.Formation and Characteristics of Nanoparticles" Macromolecules. 26. 3062-3068 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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