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GENE TRANSFORMATION EXPERIMENT OF THE SELF-INCOMPATIBILITY RELATED GENES

Research Project

Project/Area Number 05556002
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Breeding science
Research InstitutionTOHOKU UNIVERSITY

Principal Investigator

HINATA Kokichi  TOHOKU UNIVERSITY,FACULTY OF AGRICULTURE,PROFESSOR, 農学部, 教授 (00005589)

Co-Investigator(Kenkyū-buntansha) WATANABE Masao  TOHOKU UNIVERSITY,FAC.OF AGRICULTURE,ASSIS.RESEARCHER., 農学部, 助手 (90240522)
TORIYAMA Kinya  TOHOKU UNIVERSITY,FAC.OF AGRICULTURE,ASSOC.PROFESSOR., 農学部, 助教授 (20183882)
NAKANISHI Tetsu  KOBE UNIVERSITY,DEPARTMENT OF NATURAL SCIENCE,PROFESSOR., 大学院・自然科学研究科, 教授 (80031227)
SAKIYAMA Fumio  OSAKA UNIVERSITY,INSTITUTE FOR PROTEIN RESEARCH,PROFESSOR., 蛋白質研究所, 教授 (40029947)
ISOGAI Akira  NARA ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY,GRADUATE SCHOOL OF BIOLOGICAL, バイオサイエンス研究科, 教授 (20011992)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥16,600,000 (Direct Cost: ¥16,600,000)
Fiscal Year 1995: ¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1994: ¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 1993: ¥9,700,000 (Direct Cost: ¥9,700,000)
KeywordsSELF-INCOMPATIBILITY / BRASSICA CAMPESTRIS / PYRUS PILIFOLIA / SLG / SRK / S-RNASE / GENE-MANIPULATION / TRANSGENIC PLANT / S遺伝子 / RNase / アブラナ科 / ニホンナシ / クローニング / 二十世紀ナシ
Research Abstract

Objectives : To understand the molecular mechanism of self-incompatibility and to assess a possibility of molecular breeding of this character, molecular characterization and gene manipulation was conducted on the S-related genes in sporophytic self-incompatible plant : Brassica campestris and in gemetophytic self-incompatible plant : Pyrus pilifolia.
Results : In B.campestris, SLG8, SLG9, SLG12, SRK8, SRK9, SRK12, SRA1, SRA2, SRA3, SRA4 and SRBs were isolated from cDNA or genomic DNA libraries and sequenced. Dendrograms constructed on the basis of synonymous and nonsynonymous substitutions indicated that SRA differentiated first, followed by SRB and SLG/SRK.When the SLG8 genomic clone was introduced into B.campestris some of transformed plants changed to self-compatible. Furthermore, the antisense SLG gene was transformed into the same species. Analysis of a transformant showed that the self-incompatibility was broken down by the action of the introduced antisense gene. These results i … More ndicated the self-incompatibility could be controlled, at least partly, by gene manipulation. The genes encoding 3 RNases were cloned from the style of a self-incompatible cultivar, Nijisseiki (S2S4), and its self-compatible mutant, Osa-Nijisseiki (S2S4sm, sm means stylar part mutant), of Japanese pear. For Nijisseiki, cDNAs coding for two S-RNases (S2-RNase and S4-RNase) and an RNase unrelated to self-incompatibility (non-S-RNase) were cloned from the stylar cDNA library. However, the cDNA coding for S4-RNase was neither amplified by PCR nor cloned from the library of Osa-Nijisseiki, suggesting that the mutation of self-incompatible Nijisseiki to self-compatible Osa-Nijisseiki is due to a failure of expression of S4-RNase. The S3-gene promoter of Petunia inflata was used in constructing the vectors for the Japanese pear S-genes. The binary vectors of the Agrobacterium were transformed in the petunia resulting in shoot regeneration. In vitro cultures of the Japanese pear were also extended. Less

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (68 results)

All Other

All Publications (68 results)

  • [Publications] Yamakawa, S., Watanabe, M., Isogai, A., Takayama, S., Satoh, S., Hinata, K. and Suzuki, A.: "The cDNA sequence of NS3-glycoprotein from Brassica campestris and its homology to related proteins." Plant Cell Physiol.34. 173-175 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nou, I. S., Watanabe, M., Isuzugawa, K., Isogai, A. and Hinata, K.: "Isolation of S-allele from an wild population of Brassica compestris L. at Balcesme, Turkye and their characterization by S-glycoprotein." Sex. Plant Reprod.6. 71-78 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nou, I. S., Watanabe, M., Isogai, A. and Hinata, K.: "Comparision of S-alleles and S-glycoproteins between wild populations of Brassica campestris in Turkey and Japan." Sex. Plant Reprod.6. 79-86 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hinata, K., Watanabe, M., Toriyama, k. and Isogai, A.: "A review of recent studies on homomorphic self-incompatibility." Inter. Rev. Cytol.143. 257-296 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wong, K. C., Watanabe, M. and Hinata, K.: "Protein profiles in pin and thrum floral organs of distylous Averrhoa carambola L." Sex. Plant Reprod. 7. 7. 107-115 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wong, K. C., Watanabe, M. and Hinata, K.: "Fluorescnese and scanning electron microscopic studies on self-incompatibility in distylous Averrhoa carambola L." Sex. Plant Reprod.7. 116-121 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamakawa, S., Shiba, H., Watanabe, M., Shiozawa, H., Takayama, S., Hinata, K., Isogai, A. and Suzuki, A.: "The sequence of S-glycoproteins involved in self-incompatibility of Brassica campestris and their distribution among Brassicaceae." Biosci. Biotech. Biochem.58. 921-925 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Watanabe, M., Takasaki, T., Toriyama, K., Yamakawa, S., Isogai, A., Suzuki, A. and Hinata, K.: "A high degree of homology exists between the protein encoded by SLG and the S recept or domain encoded by SRK in self-incompatible Brassica campestris L." Plant Cell Physiol.35. 1221-1229 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hinata, K., Watanabe, M., Isogai, A., Yamakawa, S., Satta, Y. and Takahata, N.: "Genetic studies of self-incompatible Brassica campestris." “Pollen-pistil interactions and pollen tube growth (Current Topics in Plant Physiology : An American Society of Plant Physiologists Series.). 12. 103-110 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsushita, M., Watanabe, M., Yamakawa, S., Isogai, A. and Hinata, K.: "Base sequence variation of the S-glycoprotein within the same S12-allele in Brassica campestris." "Pollen-pistil interactions and pollen tube growth (Current Topics in Plant Physiology : An American Society of Plant Physiologists Series.). 12. 313-315 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamakawa, S., Watanabe, M., Hinata, K., Suzuki, A. and Isogai, A.: "The sequences of S-receptor kinase (SRK) involved in self-incompatibility and their homologies to S-locus glycoprotein of Brassica campestris." Biosci. Biotech. Biochem.59. 161-162 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hinata, K., Watanabe, M., Yamakawa, S., Satta, Y. and Isogai, A.: "Evolutionary aspects of the S-related genes of the Brassica self-incompatibility system : synonymous and nonsynonyomous base substitutions." Genetics. 140. 1099-1104 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzuki, G., Watanabe, M., Toriyama, K., Isogai, A. and Hinata, K.: "Molecular cloning of members of the S-multigene family in self-incompatible Brassica campestris L." Plant Cell Physiol.36. 1273-1280 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shinba, H., Hinata, K., Suzuki, A. and Isogai, A.: "Breakdown of self-incompatibility in Brassica by the antisense RNA of SLG gene." Proc. Japan Academy,. 71B. 81-83 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroda, S., Norioka, S., Mitta, M., Kato, I., and Sakiyama, F.: "Primary structure of a novel stylar RNaseunassociated with self-incompatibility in tobacco plant, Nicotiana alata." J. Protein Chem.13. 438-439 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ishimizu, T., Miyagi, M., Norioka, S., Ying-Hong Liu, Clarke, A. E. and Sakiyama, F.: "Identification of histidine 31 and cysteine 95 in the active site of self-incompatibitlity associated S6-RNase in Nicotiana alata." J. Biochem.118. 1007-1013 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Norioka N., Ohnishi Y., Norioka S., Ishimizu T., Nakanishi, T. & Sakiyama F.: "Nucleotide Sequences of cDNAs Encodinf S2-and S4-RNases from Japanese Pear (Pyurs pyrifolia Nakai)" Plant Physiol.108. 1343 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 渡辺正夫・鳥山欽哉: "花粉学事典(日本花粉学会編,分担執筆)" 朝倉書店, (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hinata, K., Isogai, A. and Isuzugawa, K.,: "Genetic control of self-incompatibility and reproductive development in flowering plants" eds. by Williams, E. G., Clarke, A. E. and Knox, R. B.," Kluwer Academic Publishers,102-115 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamakawa, S., Watanabe, M., Isogai, A., Takayama, S., Satoh, S., Hinata, K.and Suzuki, A.: "The cDNA sequence of NS3-glycoprotein from Brassica campestris and its homology to related proteins" Plant Cell Physiol. 34(1). 173-175 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nou, I.S., Watanabe, M., Isuzugawa, K., Isogai, A.and Hinata, K.: "Isolation of S-allele from a wild population of Brassica campestris L.at Balcesme, Turkey and their characterization by S-glycoprotein." Sex. Plant Reprod. 6(1). 71-78 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Nou, I.S., Watanabe, M., Isogai, A.and Hinata, K.: "Comparison of S-alleles S-glycoproteins between wild populations of Brassica campestris in Turkey and Japan." Sex. Plant Reprod. 6(2). 79-86 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hinata, K., Watanabe, M., Toriyama, K.and Isogai, A.: "A review of recent studies on homomorphic self-incompatibility." Intern. Rev. Cytol. 143. 257-296 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wong, K.C., Watanabe, M.and Hinata, K.: "Protein profiles in pin and thrum floral organs of distylous Averrhoa carambola L." Sex. Plant Reprod. 7(2). 107-115 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Wong, K.C., Watanabe, and Hinata, K.: "Fluorescnese and scanning electron microscopic studies on self-incompatibility in distylous Averrhoa carambola L." Sex. Plant Reprod. 7(2). 116-121 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamakawa, S., Shiba, H., Watanabe, M., Shiozawa, H., Takayama, S., Hinata, K., Isogai, A.and Suzuki, A.: "The sequence of S-glycoproteins involved in self-incompatibility of Brassica campestris and their distribution among Brassicaceae." Biosci. Biotech. Biochem. 58(5). 921-925 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Watanabe, M., Takasaki, T., Toriyama, K., Yamakawa, S., Isogai, A., Suzuki, A.and Hinata, K.: "A high degree of homology exists between the protein encoded by SLG and the S receptor domain encoded by SRK in self-incompatible Brassica campestris L." Plant Cell Physiol. 35(8). 1221-1229 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hinata, K., Watanabe, M., Isogai, A., Yamakawa, S., Satta, Y.and Takahara, N.: "Genetic studies of self-incompatible Brassica campestris" In "Pollen-pistil interactions and pollen tube growth (Current Topics in Plant Physiology : An American Society of Plant Physiologists Series.)". 12. 103-110 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Matsushita, M., Watanabe, M., Yamakawa, S., Isogai, A., and Hinata, K.: "Base sequence variation of the S-glycoprotein within the same S12-allele in Brassica campestris." In "Pollen-pistil interactions and pollen tube growth (Current Topics in Plant Physiology : An American Society of Plant Physiologists Series.)". 12. 313-315 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamakawa, S., Watanabe, M., Hinata, K., Suzuki, A.and Isogai, A.: "The sequences of S-receptor kinase (SRK) involved in self-incompatibility and their homologies to S-locus glycoprotein of Brassica campestris" Biosci. Biotech. Biochem.59(1). 161-162 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hinata, K., Watanabe, M., Yamakawa, S., Satta, Y., and Isogai, A.: "Evolutionary aspects of the S-related genes of the Brassica self-incompatibility system : synonymous and nonsynonyomous base substitutions." Genetics. 140. 1099-1104 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Suzuki, G., Watanabe, M., Toriyama, K., Isogai, A.and Hinata, K.: "Molecular cloning of members of the S-multigene family in self-incompatible Brassica campestris L." Plant Cell Physiol. 36(7). 1273-1280 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Hinata, K., Isogai, A.and Isuzugawa, K.: "Manipulation of sporophytic self-incompatibility in plant breeding" In "Genetic control of self-incompatibility and reproductive development in flowering plants" eds. by Williams, E.G., Clarke, A.E.and Knox, R.B., Kluwer Academic Publishers. 540. 102-115 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Shiba, H., Hinata, K., Suzuki, A.and Isogai, A.: "Breakdown of self-incompatibility in Brassica by the antisense RNA of SLG gene." Proc. Japan Academy. 71, Sr. B. 81-83 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Kuroda, S., Norioka, S., Mitta, M., Kato, I., and Sakiyama, F.: "Primary structure of a novel stylar RNaseunassociated with self-incompatibility in tobacco plant, Nicotiana alata" J.Protein Chem. 13. 438-439 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Ishimizu, T., Miyagi, M., Norioka, S., Ying-Hong Liu, Clarke, A.E.and Sakiyama, F.: "Identification of histidine 31 and cysteine 95 in the active site of self-incompatibility associated S6-RNase in Nicotiana alata" J.Biochem. 118. 1007-1013 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Norioka, N., Ohnishi, Y., Norioka, S., Ishimizu, T., Nakanishi, T.and Sakiyama F.: "Nucleotide Sequences of cDNAs Encodinf S2-and S4-RNases from Japanese Pear (Pyrus pyrifolia Nakai)" Plant Physiol. 108. 1343 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Yamakawa, S,, Watanabe, M., Isogai A., Takayama, S., Satoh, S., Hinata, K. and Suzuki, A.: "The cDNA sequence of NS3-glycoprotein from Brassica campestris and its homology to related proteins." Plant Cell Physiol.34. 173-175 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nou, I. S., Watanabe, M., Isuzugawa, K., Isogai, A., and Hinata, K.: "Isolation of S-allele from a wild population of Brassica campestris L. at Balcesme, Turkey and their characterization by S-glycoprotein." Sex. Plant Reprod.6. 71-78 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Nou, I. S., Watanabe, M., Isogai, A., and Hinata, K.: "Comparison of S-alleles and S-glycoproteins between wild populations of Brassica cam pestris in Turkey and Japan." Sex. Plant Reprod. c. 6. 79-86 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hinata, K., Watanabe, M., Toriyama, K,, and Isogai, A.: "A review of recent studies on homomorphic self-incompatibility." Inter. Rev. Cytol.143. 257-296 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] Wong, K. C., Watanabe, M. and Hinata, K.: "Protein profiles in pin and thrum floral organs of distylous Averrhoa carambola L." Sex. Plant Reprod. 7. 7. 107-115 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Wong, K. C., Watanabe, M. and Hinata, K.: "Fluorescnese and scanning electron microscopic studies on self-incompatibility in disty lous Averrhoa carambola L." Sex. Plant Reprod.7. 116-121 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamakawa, S., Shiba, H., Watanabe, M., Shiozawa, H., Takayama, S., Hinata, K., Isogai, A. and Suzuki, A.: "The sequence of S-glycoproteins involved in self-incompatibility of Brassica campestris and their distribution among Brassicaceae." Biosci. Biotech. Biochem.58. 921-925 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Watanabe, M., Takasaki, T., Toriyama, K., Yamakawa, S., Isogai, A., Suzuki, A. and Hinata, K.: "A high degree of homology exists between the protein encoded by SLG and the S recept or domain encoded by SRK in self-incompatible Brassica campestris L." Plant Cell Physiol.35. 1221-1229 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hinata, K., Watanabe, M., Isogai, A., Yamakawa, S., Satta, Y. and Takahata, N.: "Genetic studies of self-incompatible Brassica campestris." “Pollen-pistil interactions and pollen tube growth (Current Topics in Plant Physiology: An American Society of Plant Physiologists Series. ). 12. 103-110 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Matsushita, M., Watanabe, M., Yamakawa, S., Isogai, A. and Hinata, K.: "Base sequence variation of the S-glycoprotein within the same S12-allele in Brassica campestris." “Pollen-pistil interactions and pollen tube growth (Current Topics in Plant Physiology: An American Society of Plant Physiologists Series. ). 12. 313-315 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Yamakawa, S., Watanabe, M., Hinata, K., Suzuki, A. and Isogai, A.: "The sequences of S-receptor kinase(SRK)involved in self-incompatibility and their homologies to S-locus glycoprotein of Brassica campestris." Biosci. Biotech. Biochem.59. 161-162 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hinata, K., Watanabe, M., Yamakawa, S., Satta, Y. and Isogai, A.: "Evolutionary aspects of the S-related genes of the Brassica self-incompatibility system:synonymous and nonsynonyomous base substitutions." Genetics. 140. 1099-1104 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Suzuki,G.,Watanabe, M., Toriyama, K., Isogai, A. and Hinata, K.: "Molecular cloning of members of the S-multigene family in self-incompatible Brassica campestris L." Plant Cell Physiol.36. 1273-1280 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Shinba, H., Hinata, K., Suzuki, A. and Isogai. A.: "Breakdown of self-incompatibility in Brassica by the antisense RNA of SLG gene." Proc. Japan Academy,. 71B. 81-83 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Kuroda, S., Norioka, S., Mitta, M., Kato, I., and Sakiyama, F.: "Primary structure of a novel stylar RNaseunassociated with self-incompatibility in tobacco plant, Nicotiana alata." J. Protein Chem.13. 438-439 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Ishimizu, T., Miyagi, M., Norioka, S., Ying-Hong Liu, Clarke, A. E. and Sakiyama, F.: "Identification of histidine 31 and cysteine 95 in the active site of self-incompatibility a ssociated S6-RNase in Nicotiana alata." J. Biochem.118. 1007-1013 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Norioka, N., Ohnishi, Y., Norioka, S., Ishimizu, T., Nakanishi, T. & Sakiyama F.: "Nucleotide Sequences of cDNAs Encodinf S2-and S4-RNases from Japanese Pear(Pyrus pynfolia Nakai)" Plant Physiol.108. 1343- (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 渡辺正夫・鳥山欽哉: "花粉学事典(日本花粉学会編,分担執筆)" 朝倉書店, (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Hinata, K., Isogai, A. and Isuzugawa, K.,: "Genetic control of self-incompatibility and reproductive development in flowering plants" eds. by Williams, E. G., Clarke, A. E. and Knox, R. B.," Kluwer Academic Publishers,102-115 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] MASAO WATANABE: "A high degree of homology exists between the protein encoded by SLG and the S-receptor domain encoded by SRK in celf-incompatible B.comper Trial," Plant and Cell Physiology. 35. 1221-1229 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Seyei YAMAKAWA: "The sequence of S-glycoproteins insolved in self-incompatibility of Brossica campustri and their distribution among Brossicaceae" Bioscience Biotechnology and Biochemistry. 58. 921-925 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Seyei YAMAKAWA: "The sequences of S-receptorkinases(SRK) insolved in self-incompatibility and their hemologies the Slecus glycoprotein" Bioscience Biotechnology and Biochemistry. 59. 161-162 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] SATOSHI KURODA: "Primary structive of a novel stylar RNase an associated with self-incompatibility in tabacco plant N.alata" Journal of Protein Chemistry. 438-439 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 崎山文夫: "雌芯のリボヌクレアーゼと自家不和合性" 蛋白質・核酸・酵素. 40. 293-303 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Kokichi HINATA: "A Review of receut studies on homomrphic self-incompatibility" International Review of Cytology. 143. 257-296 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.YAMAKAWA: "S-glycoproteins in volved in self-incompatibility of Brassica campestris and their distribution among Brassicaceae" Biosci.Biotech.Biochem.(印刷中). (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] F.SAKIYAMA: "RNase in the style of self-incompotible and self-compotible Japanese pear." Abstracts of XVth Intem.Botanical Congress. 176 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 小野享: "Brassica campestrisの自家不和合性系統yellow sarsonの自家和合性の遺伝分析" 育種学雑誌(別冊2). 43. 306 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 渡辺正夫: "自家不和合性Brassica campestrisにおけるS-レセプタープロテインキナーゼ(SRK)のクローニングと遺伝子発現" 育種学雑誌(別冊2). 43. 308 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.TAKASAKI: "High frequency shoot regeueration system for Agrobacterium-mediated trausformation in Brassica campestris" Abstracts of XVth Intern.Botamical Congress. 536 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.WATANABE: "Moleculeir characterizatin of SRK and SLG geues of self-incompatible Brassica campestris" Abstracts of XVth Intern.Botamical Congress. 449 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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