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Continuous Production of Silkworm Polyvoltine Strain Eggs by Ideal Analogs of Diapause Hormone

Research Project

Project/Area Number 05556008
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 蚕糸・昆虫利用学
Research InstitutionNagoya University

Principal Investigator

YAMASHITA Okitsugu  Nagoya Univ.Fac.Agric.Professor, 農学部, 教授 (50023411)

Co-Investigator(Kenkyū-buntansha) TAKEDA Reiji  Shionogi Pharmaceutica Co.Ltd.Aburahi Lab.Derctor, 油日ラボラトリーズ, 研究員
FURUSAWA Toshiharu  Kyoto Institute of Technology Department of Applied Biology Professor, 繊維学部, 教授 (70127166)
SATO Yukihiro  Nagoya Univ. Radioisotope Research Center Assist.Professor, アイソトープ総合センター, 助手 (60222022)
IMAI Kunio  Mie Univ.Fac.Bioresorces Assoc.Professor, 生物資源学部, 助教授 (80109313)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥15,100,000 (Direct Cost: ¥15,100,000)
Fiscal Year 1994: ¥5,100,000 (Direct Cost: ¥5,100,000)
Fiscal Year 1993: ¥10,000,000 (Direct Cost: ¥10,000,000)
KeywordsDiapause hormone / Diapause hormone analog / Diapause hormone gene / gene expression / neurosecretory cell / diapause egg / Bombyx mori / 修飾ペプチド / 構造活性相関 / 多化性系統
Research Abstract

A neuropeptide hormone, diapause hormone (DH) is a 24 amino acid peptide amide which is responsible for induction of embryonic diapause of the silkworm, Bombyx mori. DH or its analog is a novel chemical agent to be useful for long term preservation of eggs of the polyvoltine strain of the silkworm. In order to develop some active analog of DH,we chemically synthesized more than 100 species of DH fragment analogs, and tested their diapause egg inducing activity. The result led con-clusions that the amidation of C terminus and the C-terminal pentapeptide sequence, FXPRL, are essential for expression of biological activity of DH.Thus, the C-terminal hexa peptide amide seemed to be an agonist of DH.
To obtain a new DH-like peptide which is lipophilic and permeable through integment of silkworm pupae, we extracted DH active substances from male adult heads by using an organic solvent. The active materials were purified, and one of them was isolated. The isolated substance was a peptide with a MW of about 10K,and its N-terminal amino acid sequencing showed high content of Val, Ala andPro. The peptide was weak in biological activity, but acted as a protectant against DH degradation.
DH gene was organized with 6 exons interrupted by 5 introns, and was expressed in 12 neurosecretory cells of suboesophageal ganglion. The incubation temperarture of eggs strictly affected the expression of the gene, which indicates that the environmental regulation of diapause is mediated through DH gene expression.
The chemically synthesized DH or its fragments was injected into pupae, by which treatment diapause eggs were obtained. These eggs accumulated much amounts of sorbitol and alanine as the natural diapause eggs. Thus, the DH agents are promising chemical regulators for long term preservation of eggs of polyvoltine strains of the silkworm.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (46 results)

All Other

All Publications (46 results)

  • [Publications] Ikeda,M. et al.: "Induction of embryonic diapause and stimulation of ovary trehalase activity in the silkworm, Bombyx mori, by synthetic diapause hormone." J. Insect Physiol.39. 889-895 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Nachman,R.J. et al.: "Silkworm diapause induction activity of myotropic pyrokinin insect neuropeptides." Peptide. 14. 1043-1048 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato,Y. et al.: "Precursor polyprotein for multipele neuropeptides secreted from the suboesophageal ganglion of the silkworm Bombyx mori: Characterization of the cDNA encoding the diapause hormone precursor and identification ofadditional peptides." Proc. Natl. Acad. Sci.USA,. 90. 3251-3255 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Su,Z.-H. et al.: "Purification, cDNA colning and Northern blot analysis of trehalase of pupal midgut of the silkworm, Bombyx mori." Biochim. Biophys. Acta,. 1173. 217-224 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 古沢 寿治ら: "天蚕卵の休眠に伴うリン脂質構成脂肪酸の変化." 日蚕雑,. 63. 57-63 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Furusawa et al.: "Low temperature-induced accumulation of alanine in the hemolymph of diapausing pre-larvae and its utilization during development of Antheraea yamamai." Int. J. Wild Silkmoth & Silk,. 1. 31-35 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Saito,H. et al.: "The core and complementary sequence responsible for biological activity of the diapause hormone of the silkworm, Bombyx mori." Peptide,. 15. 1173-1178 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato,Y. et al.: "Neurosecretory cells expressing the gene for common precursor for diapause hormone and pheromone biosynthesis-activating neuropeptide in the suboesophageal ganglion of the silkworm, Bombyx mori." Gen. Comp. Endocrinol.96. 27-36 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shiomi,K. et al.: "Induction of non-diapause eggs by injection of anti-diapause hormone rabbit serum into the diapause type of the silkworm, Bombyx mori." J. Insect Physiol.40. 693-699 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Su,Z.-H. et al.: "Molecular characterization of ovary trehalase of the silkworm, Bombyx mori and its transcriptional activation by diapause hormone." Biochim. Biophys. Acta. 1218. 366-374 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamashita,O.: "Diapause hormone of the silkworm, Bomyx mori: chemical identification and gene expression." Sericologia. 33. 411-419 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Xu,W.-H. et al.: "Stage-dependent and temperature-controlled expression of the gene encoding the precurosr protein of diapause hormone and pheromone biosynthesis activating neuropeptide in the silkworm, Bombyx mori." J. Biol. Chem.,. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Xu,W.H.et al: "Molecular characterization of the silkworm,Bombyx mori andits transcriptional activation by diapause hormone." Biochim.Biophys.Acta in press.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 山下 興亜(小林ら編): "『シルクのはなし』" 技報堂出版, 6 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 山下 興亜(竹田ら編): "『昆虫の季節適応と休眠』" 文一総合出版, 10 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Furusawa, T.et al.: "Changes in amino acid pools during embryonic development of the Japanese oak silkworm, Anthereae yamamai (Lepidoptera : Sturniidae)." Appl.Entomol.Zool.28. 234-237 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Furusawa, T.et al.: "Stage-dependent limited degradation of vitellin and its localization in the Japanese oak silkworm, Anthereae yamamai, during embryonic development." Comp.Biochem.Physiol.104B. 787-794 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Furusawa, T.et al.: "Double-stranded ribonuclease activity in the digestive juice and midgut homogenate of the silkworm, Bombyx mori." Comp.Biochem.Physiol.104B. 795-801 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Furusawa, T.et al.: "Effect of imidazole compund, KK-42, on changes in trehalose content and trehalase activity in the silkland of the silkworm, Bombyx mori." Appl.Entomol.Zool.28. 169-176 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato, Y.et al.: "Precursor polyprotein for multiple neuropeptides secreted from the suboesophageal ganglion of the silkworm Bombyx mori : characterization of the cDNA encoding the diapause hormone precursor and identification of additional peptides." Proc.Natl.Acad.Sci.USA. 90. 3251-3255 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Furusawa et al.: "Low temperature-induced accumulation of alanine in the hemolymph of diapausing pre-larvae and its utilization during development of Anthereae yamamai." Int.J.Wild Silkmoth&Silk. 1. 31-35 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Saito, H.et al.: "The core and complementary sequence responsible for biological activity of the diapause hormone of the silkworm, Bombyx mori." Peptide. 15. 1173-1178 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Sato, Y.et al.: "Neurosecretory cells expressing the gene for common precursor for diapause hormone and pheromone biosythesis-activating neuropeptide in the suboesophageal ganglion of the silkworm, Bombyx mori." Gen.Comp.Endocrinol.96. 27-36 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shiomi, K.et al.: "Induction of non-diapause eggs by injection of antidiapause hormone rabbit serum into the diapause type of the silkworm, Bombyx mori." J.Insect Physiol.40. 693-699 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Su, Z.-H.et al.: "Molecular characterization of ovary trehalase of the silkworm, Bombyx mori and its transcriptional activation by diapause hormone." Biochim.Biophys.Acta.1218. 366-374 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Yamashita, O.: "Diapause hormone of the silkworm, Bomyx mori : chemical identification and gene expression." Sericologia. 33. 411-419 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Xu, W.-H.et al.: "Stage-dependent and temperature-controlled expression of the gene encoding the precurosr protein of diapause hormone and pheromone biosynthesis activating neuropeptide in the silkworm, Bombyx mori." J.Biol.Chem.(in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Xu, W.-H.et al.: "Molecular characterization of the gene encoding the precursor protein of diapause hormone and pheromone biosynthesis activating neuropeptide of the silkworm, Bombyx mori." Biochim.Biophys.Acta. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 古沢寿治ら: "天蚕卵の休眠に伴うリン脂質構成脂肪酸の変化." 日蚕雑,. 63. 57-63 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Furusawa et al.: "Low temperature-induced accumulation of alanine in the hemolymph of diapausing pre-larvae and its utilization during development of Antheraca yamamai." Int.J.Wild Silkmoth & Silk,. 1. 31-35 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Saito,H.et al.: "The core and complementary sequence responsible for biological activity of the diapause hormone of the silkworm,Bombyx mori." Peptide,. 15. 1173-1178 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Sato,Y.et al.: "Neurosecretory cells expressing the gene for common precursor for diapause hormone and pheromone biosynthesis-activating neuropeptide in the suboesophageal ganglion of the silkworm,Bombyx mori." Gen.Comp.Endocrinol.96. 27-36 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shiomi,K.et al.: "Induction of non-diapause eggs by injection of anti-diapause hormone rabbit serum into the diapause type of the silkworm,Bombyx mori." J.Insect Physiol.40. 693-699 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Su,Z.-H.et al.: "Molecular characterization of ovary trehalase of the silkworm,Bombyx mori and its transcriptional activation by diapause hormone." Biochim.Biophys.Acta. 1218. 366-374 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Yamashita,O.: "Diapause hormone of the silkworm,Bomyx mori.chemical identification and gene expression." Sericologia. 33. 411-419 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Xu,W.-H.et al.: "Stage-dependent and temperature-controlled expression of the gene encoding the precurosr protein of diapause hormone and pheromone biosynthesis activating neuropeptide in the silkworm,Bombyx mori." J.Biol.Chem.(in press.).

    • Related Report
      1994 Annual Research Report
  • [Publications] Xu,W.-H.et al.: "Molecular characterization of the gene encoding the precursor protein of diapause hormone and pheromone biosynthesis activating neuropeptide of the silkworm,Bombyx mori." Biochim.Biophys.Acta. (in press.).

    • Related Report
      1994 Annual Research Report
  • [Publications] Ikeda,M.et al.: "Induction of embryonic diapause and stimulation of ovary trehalase activity in the silkworm,Bombyx mori,by synthetic diapause hormone." J.Insect Physiol.39. 889-895 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Nachman,R.J.et al.: "Silkworm diapause induction activity of myotropic pyrokinin insect neuropeptides." Peptide. 14. 1043-1048 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Sato,Y.et al.: "Precursor polyprotein for multipele neuropeptides secreted from the suboesophageal ganglion of the silkworm Bombyx mori:Characterization of the cDNA encoding the diapause hormone precursor and identification ofadditional peptides." Proc.Natl.Acad.Sci.USA. 90. 3251-3255 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Su,Z.-H.,Sato,Y.,and Yamashita,O.: "Purification,cDNA cloning and Northern blot analysis of trehalase of pupal midgut of the silkworm,Bombyx mori." Biochim.Biophys.Acta. 1173. 217-224 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Saito,H.et al.: "The core and complementary sequence responsible for biological activity of the diapause hormone of the silkworm,Bombyx mori." Peptide. in press. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shiomi,K.et al.: "Induction of non-diapause eggs by anti-diapause hormone rabbit serum injected into the diapause type of the silkworm,Bombyx mori." J.Insect Physiol. in press. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] Su,Z.-H.et al.: "Molecular characterization of ovary trehalase of the silkworm,Bombyx mori and its transcriptional activation by diapause hormone." Biochim.Biophys.Acta. in press. (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 山下興亜: "シルクのはなし(編者:小林勝利)(催眠薬で休眠:卵休眠とホルモンの正体)" 技報堂出版(東京), 179(58-63) (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 山下興亜: "昆虫の季節適応と休眠(編者:竹田真木生・田中誠二)(カイコの卵休眠と休眠ホルモン)" 文一総合出版(東京), 450(338-347) (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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