• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Biochemical production of liquid alkanes from biomasses

Research Project

Project/Area Number 05556017
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用微生物学・応用生物化学
Research InstitutionFUKUYAMA Univ.

Principal Investigator

FUKUI Sakuzo  Univ.FUKUYAMA,Dept.Biotech., Prof., 工学部, 教授 (60013299)

Co-Investigator(Kenkyū-buntansha) MIYAKAWA Tokichi  HIROSHIMA,Univ., Dept.Biotech., Prof., 工学部, 教授 (10116676)
MATSUZAKI Hiroaki  FUKUYAMA,Univ., Dept.Biotech., Res.Assoc., 工学部, 助手 (90222299)
HATANO Takushi  FUKUYAMA,Univ., Dept.Biochem., Assoc.Prof., 工学部, 助教授 (60198752)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥11,900,000 (Direct Cost: ¥11,900,000)
Fiscal Year 1995: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1994: ¥4,400,000 (Direct Cost: ¥4,400,000)
Fiscal Year 1993: ¥6,100,000 (Direct Cost: ¥6,100,000)
KeywordsLiquid Alkanes / Penicillium decumbens / Secretive Production of Lipids / Fatty Acid Metabolism / n-Undecane-2-one / Agro-Petrol / バイオリアクター / アルカン醗酵 / ペニシリウム・デカンベンス / 植物石油
Research Abstract

(1) For production of liquid alkanes, agro-petrol, from middle-chain fatty acids in biomass, we established a novel fermentation process in which Penicillium decumbens IFO 7091 was used as microorganism. When parm-kernel oil was used as substrate in the process, the solid substrate was converted into liquid product. The product consisted of n-undecane-2-one, n-nonane-2-one and other n-alkane-2-ones, these are all liquid alkanes. The bioconversion formula was demonstrated from stoichiometric analysis of the substrate consumed and the product formed as follows :
Triacylglycerol*3 CH_3 (CH_2) _<n+2>COOH*3 CH_3 (CH_2) _nCOCH_3 + 3 CO_2 fatty acids n-alkane-2-ones
Lauric acid is a major constituent of parm-kernel oil, so n-undecane-2-one is the major product.
(2) n-Alkane-2-ones accumulated were then oxidized to monohydroxy-n-alkane-2-ones. The oxidation was subterminal hydroxylation, such as omega2, omega3, omega4 and omega5 hydroxylations depending on alkane chain length. Further, oxidation … More of monohydroxy-n-alkane-2-ones took place with release of 2-C or 3-C unit to give keto-ol or diol compounds. These findings indicated that fatty acds are metabolized by another oxidation system rather than so-called beta-oxidation.
(3) In order to establish an attractive bioreactor system for "agro-petrol" production, characterization and primary selection of catalytic units for conversion of fatty acids to n-alkanes were carried out. The catalytic unit was found to specifically concentrate in membrane-wall fraction of P.decumbens. For full expression of the activity, Ca^<2+> ions was required. Immobilized catalytic unit (wall-membrane fraction) in Ca-alginate-gel and polyacrylamide-gel was demonstrated as active reacter for long-term (7 days), however, gel barrier depresses substrate-product diffusion with subsequent decrease in reaction rate.
(4) For commercial supplyment of agro-petrol, raw materials, triacylglycerol and fatty acids, must be low price. Recently, We achieved the breedings of a triacylglycerol-producible yeast with secretive fashion by using glucose, an abundant biomass. Less

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] 福井作蔵: "最近における酵母との交際-n-液体アルカンの生産" 日本イ-スト工業技術報告. 63. 61-66 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T. Yagi 他: "Extracellular production of palmitoleie Triglyceride by a yeast,Trichospron sp." J. Ferment. Bioeng.,. 77. 164-168 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 福井作蔵: "燃料脂質を分泌生産する微生物の選択・育種" Bio-Industry. 12. 36-46 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y. Nojima 他: "Extracellular formation of triglycerides from glucose by a mutant strain of Trichosporon" J. Ferment. Bioeng.,. 78. 88-90 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 福井作蔵: "発酵法による液体燃料の生産は可能か-脂質の分泌生産" 油化学. 40. 835-842 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Fukui: "Recent Studies on Yeast Engineering-Production of Liquid n-Alkanes by Fungi." Techical Rep.Japan Yeast Ind.Assoc.63. 61-66 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] T.Yagi et al.: "Extracellular Production of Palmitoleic Triglycerides by a Yeast, Trichosporon." J.Ferment.Bioeng.77. 164-168 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Y.Nojima et al.: "Extraformation of Triglycerides from Glucose by a Mutant Strain of Trichosporon." J.Ferment.Bioeng.80. 88-90 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Fukui: "Breedings of Biofuel-Secretive Microorganisms." Bio-Industry. 12. 36-46 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] S.Fukui: "Production of liquid alkanes by fermentation processes." Yukagaku. 40. 835-842 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 福井作蔵: "最近における酵母との交際-n-液体アルカンの生産" 日本イ-スト工業会技術報告. 63. 61-66 (1993)

    • Related Report
      1995 Annual Research Report
  • [Publications] T. Yagi: "Extracellular Production of Palmitoleic Triglyceride by a Yeast, Trichosporon SP," J. Fermentation and Bioengineering. 77. 164-168 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 福井作蔵: "燃料脂質を分泌生産する微生物の選択・育種" Bio-Industry. 12(3). 36-46 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Y. Nojima: "Extracelluler Formation of Triglycerides from glucose by a Mutant Strain of Trichosporon" J. Fermentation and Bioengineering. 78. 印刷中 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 福井作蔵: "発酵法による液体燃料の生産は可能か-脂質の分泌生産" 油化学. 40. 835-842 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 福井 作蔵: "最近における酵母との交際-n-液体アルカンの生産" 日本イ-スト工業会技術報告. 63. 1-6 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.Yagi: "Extracellular Production of Palmitoleic Triglyceride by a Yeast, Trichcsporon sp." J.Fermentation and Bioengineering. 77. 164-168 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 福井 作蔵: "燃料脂質を分泌生産する微生物の選択・育種" Bio-Industry. 12(3). 36-46 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] Y.Nojima: "Extracelluler Formation of Triglycerides from glucose by a Mutant Strain of Thichosporon" J.Fermentation and Bioengineering. 78(印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] T.yagi: "Extracellular Production of Palmitoleic Triglycericie by a Yeast,Trichesporon sp." J.Fermention and Bioengineeing. 77. 164-168 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 福井 作蔵: "最近における酵母菌との交際(つきあい)-n-液体アルカンの生産" 日本イースト工業会技術報告. 63. 予定 (1994)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi