• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of Damaged DNA Markers for Prevention by Food Factors

Research Project

Project/Area Number 05556021
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 食品科学・栄養科学
Research InstitutionNagoya University

Principal Investigator

OSAWA Toshihiko  Nagoya Univ.Sch.Agr.Prof., 農学部, 教授 (00115536)

Co-Investigator(Kenkyū-buntansha) YAMASHITA Kanahe  Sugiyama Univ.Prof., 生活科学部, 教授 (40080113)
TOYOKUNI Shinya  Kyoto Univ.Sch.Med.Asc.Prof., 医学部, 講師 (90252460)
NAKAYAMA Tsutomu  Univ.Shizuoka Asc.Prof., 食品栄養科学部, 助教授 (50150199)
UCHIDA Koji  Nagoya Univ.Sch.Agr.Ass.Prof, 農学部, 助手 (40203533)
川岸 舜朗  名古屋大学, 農学部, 教授 (50023445)
Project Period (FY) 1993 – 1995
Project Status Completed (Fiscal Year 1995)
Budget Amount *help
¥13,400,000 (Direct Cost: ¥13,400,000)
Fiscal Year 1995: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1994: ¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 1993: ¥7,900,000 (Direct Cost: ¥7,900,000)
KeywordsCancer / Free radicals / Markers for diseases / 8-hydroxydeoxy-guanosine / Oxidized materials / Monoclonal antibody / 酸化的障害物質 / 脂質過酸化 / 酸素ラジカル / 4-ヒドロキシノネナ-ル / 8-ヒドロキシデオキシグアノシン / セサミノール配糖体 / ゴマ種子 / リグナン化合物 / セサミノール / モノクローナル抗体 / DNA傷害マーカー
Research Abstract

Free radical has been associated with important pathophysiological events in a variety of diseases, drug toxicities, and traumatic or ischemic injuries. It has been postulated that free radicals generated during the process may be responsible for these effects because of their ability to damage cellular membranes, proteins, and DNA.We have developed a unique immunochemical procedure for quantifying 8-oxodeoxyguanosine (8-OH-dG) generated in the oxidized DNA.Using this method, we found that sesamisol glucoside decreased the level of 8-OH-dG and considerably inhibits the hepatotoxicity of rats treated with carbon tetrachloride. Furthermore, we developed another immunochemical procedure for quantifying a cytotoxic aldehyde 4-hydroxy-2-nonenal (HNE) originated from the peroxidation of polyunsaturated fatty acide. We characterize the specificity of this assay and found that considerable amount of HNE is generated in the kidney of rats treated with a renal carcinogen ferric nitrilotriacetate. Recently, we have obtained a direct evidence that oxidatively modified low density lipoproteins (LDL) also possess a considerable amount of HNE that is capable of reacting with other molecules.

Report

(4 results)
  • 1995 Annual Research Report   Final Research Report Summary
  • 1994 Annual Research Report
  • 1993 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] Uchida,K.: "Identification of Oxidized Histidin Generated at the Active Site of Cu,Zn-Superoxide Distutase Exposed to H_2O_2" J.Biol.Chem.269. 2405-2410 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyokuni,S.: "Formation of 4-Hydroxy-2-nonenal-modified Proteins in the Renal Proximal Tubules of Rats Treated with a Renal Carcinogen,Ferric Nitrilotriacetate." Proc.Natl.Acad.Sci.U.S.A.91. 2616-2620 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Uchida,K.: "Acute Nephrotoxicity of Carcinogenic Iron Chelate:Selective Inhibition of Proteolytic Conversion of a2u-globulin to Kidney Fatty Acid-binding Protein" FEBS Lett. 357. 165-167 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyokuni,S.: "The Monoclonal Antibody Specific for the 4-Hydroxy-2-nonenal Histidine Aclduct" FEBS Lett.359. 189-191 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Uchida,K.: "4-Hydroxy-2-nonenal-trapping Elisa:Direct Evidence for the Release of a Cytotoxic Aldehyde from Oxidized Low Density Lipoproteins" Biochem.Biophys.Res.Comm.212. 1068-1073 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Uchida,K.: "A Renal Carcinogen Ferric Nitrilotriacetate Mediates a Temporary Accumlation of Aldehyde-modified Proteins within Cytosolic Compartment of Rat Kidney." Arch.Biochem.Biophys.317. 405-411 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 内田 浩二: "老化と蛋白質カルボニル" メディカルレビュー社, 80 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] 内田 浩二: "食品成分による発癌予防酵素群の誘導" シ-エムシ-出版, 346 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Uchida, K.: "Identification of Oxidized Histidin Generated at the Active Site of Cu, Zn-superoxide Dismutase Exposed to H_2O_2." J.Biol.Chem.269. 2405-2410 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyokuni, S.: "Formation of 4-Hydroxy-2-nonenal-modified Proteins in the Renal Proximal Tubules of Rats Treated with a Renal Carcinogen, Ferric Nitrilotriacetate." Proc.Natl.Acad.Sci.U.S.A. 91. 2616-2620 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Uchida, K.: "Acute Nephrotoxicity of Carcinogenic Iron Chelate : Selective Inhibition of Proteolytic Conversion of a_2v-globulin to Kidney Fatty Acid-binding Protein." FEBS Lett.357. 165-167 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Toyokuni, S.: "The Monoclonal Antibody Specitic for the 4-Hydroxy-2-nonenal Histidine Adduct." FEBS Lett.359. 189-191 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Uchida, K.: "4-Hydroxy-2-nonenal-trapping Elisa : Direct Evidence for the Release of a Cytotoxic Aldehyde from Oxidized Low Density Liporoteins." Biochem.Biophys.Res.Comm.212. 1068-1073 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Uchida, K.: "A Renal Carcinogen Ferric Nitrilotriacetate Mediates a Temporary Accumulation of Aldehyde-modified Proteins within Cytsolic Compartment of Rat Kidney" Arch.Biochem.Biophys.317. 405-411 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1995 Final Research Report Summary
  • [Publications] Vchida,K: "Identification of Oxidized Histidin Generated at the Active Site of Cu,Zn-superoxide Dismutase Exposed to H_2O_2." J.Biol.Chem.269. 2405-2410 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Toyokuni,S.: "Fromation of 4-Hydroxy-2-nonenal-modified Proteins in the Renal Proximal Tubules of Rats Treated with a Renal Carcinogen,Ferric Nitrilotriacetate," Proc.Natl.Acad.Sci.U.S.A. 91. 2616-2620 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] Uchida,K.: "Acute Nephrotoxicity of Carcinogenic Iron Chelate:Selective Inhibition of Proteolytic Conversion of azu-globulin to Kidney Fatty Acid-binding Protein." FEBS Lett.357. 165-167 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Toyokuni,S.: "The Monoclonal Antibody Specific for the 4-Hydroxy-2-nonenal Histidine Adduct." FEBS Lett.359. 189-191 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Uchida,K.: "4-Hydroxy-2-nonenal-trapping.Elisa:Direct Evidence for the Release of a Cytocoxic Aldehyde from Oxidized Low Density Lipoproteins." Biochem.Biophys Res.Comm.212. 1068-1073 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] Uchida,K.: "A Renal Carcinogen Ferric Nitrilotriacetate Mediates a Temporary Accumulation of Aldehyde-modified Proteins within Cytosolic Compartment of Rat Kidney." Arch.Biochem.Biophys.317. 405-411 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] 内田浩二: "老化と蛋白質カルボニル" メディカルレビュー社, 80 (1994)

    • Related Report
      1995 Annual Research Report
  • [Publications] 内田浩二: "食品成分による発癌予防酵素群の誘導" シ-エムシ-出版, 346 (1995)

    • Related Report
      1995 Annual Research Report
  • [Publications] T.Osawa: "Plant antioxidants:Protective vole against oxygen radical species" Cosmetics a Toiletries magazine. 109. 77-81 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] K.Uchida: "Michael addition-type 4-hgdroxy-2-nonenal adducts in modified low-density lipoproteins:Markers for Atherosclerosis" Biochemistry. 33. 12487-12494 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] S.Toyokuni: "The mouoclonal antibody specific for the 4-hgdroxy-2-nonenal histidine adduct" F EBS Lett.359. 189-191 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 大澤俊彦: "抗酸化物による老年病の予防" 老年医学. 32. 1087-1093 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] H.Katsuzaki: "Sesaminol glucosides in sesame seeds" Phytochemistry. 35. 773-776 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] M.Shiraki: "Antioxidative and antimutagenic effects of theaflaving from black tea." Mutation Research. 323. 29-34 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] K.Uchida: "Immunochemical detection of 4-hydroxy-2-nonenal-modified proteins in oxidized hepatocytes" Proc.Natl.Acad.Sci.U.S.A.90. 8742-8746 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] T.Nakayama: "The protective role of gallic acid esters in bacterial cytotoxicity and SOS responses induced by hydrogen peroxide" Mutation Research. 303. 29-34 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] S.Toyokuni: "Induction of oxidative single- and double-strand breaks in DNA by ferric citrate" Free Rad.Biol.Med.15. 117-123 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 山下 かなへ: "セサミンとトコフェロール" 化学と生物. 31. 704-706 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] M-T,Huang: "Food Phytochemicals for Cancer Prevention I" American Chemical Society, 427 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] M-T,Huang: "Food Phytochemicals for Cancer Prevention II" American Chemical Society, 370 (1994)

    • Related Report
      1993 Annual Research Report

URL: 

Published: 1993-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi