| Project/Area Number |
05557006
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental physiology (including Physical medicine and Nutritional physiology)
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| Research Institution | Osaka University |
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Principal Investigator |
KANOSUE Kazuyuki Osaka Univ., Sch.Allied Health Sci., Professor, 医学部, 教授 (50127213)
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| Co-Investigator(Kenkyū-buntansha) |
SHIONO Satoru Mitsubishi Electric Corp., Research Group Manager, 中央研究所, グループマネージャー
FUZIWARA Motoko Osaka Univ.Med.Sch., Research Associate, 医学部, 助手 (30220198)
KOSAKA Jun Osaka Univ.Med.Sch., Research Associate (40243216)
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| Project Period (FY) |
1992 – 1994
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| Project Status |
Completed (Fiscal Year 1994)
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| Budget Amount *help |
¥15,600,000 (Direct Cost: ¥15,600,000)
Fiscal Year 1994: ¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1993: ¥13,600,000 (Direct Cost: ¥13,600,000)
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| Keywords | hypothalamus / culture / optical recording |
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| Research Abstract |
Homeostatic functions such as body temperature regulation, eating, drinkig, and reproduction are controlled by the central nervous system including the hypothalamus. There is almost no knowledge concerning thermoregulatory "network" , which makes it difficult to link electrophysiological and pharmachological data directly with responses observed in whole animals. Present study was planed to develop a system which analyzes the network by simultaneously recording activities of many neurons from hypothalamic culture preparation. Neurons taken from the preoptic area of new-born rats (three days old) were dissociated and cultrured. Neurons survived for 7-10 days and at around day 7 long processes were observed. In ordere to make optical recordings from single cultured neurons, we tried to find out conditions for making the neurons generate action potentials. No spontaneous activity was observed not only at 37ﾟCbut also in a wide temperature range of 34-42ﾟC.Application of glutamate (1 mM-0.1 M) or K+ (10-100 mM) also failed to generate action potentials. Signal to noise ratio of the plesently-available optical recording system requires voltage change of several tens mV.So without action potentials optical recordings can not be used for analyzing neuronal networks of the hypothalamus.
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