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Development of the assay system for netralizing antibodies agaist hepatitis C virus

Research Project

Project/Area Number 05557021
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Virology
Research InstitutionThe University of Tokyo

Principal Investigator

IWAMOTO Aikichi  University of Tokyo, Institute of Medical Science, Professor, 医科学研究所, 教授 (10133076)

Co-Investigator(Kenkyū-buntansha) SHUIMIZU Yohko  University of Tokyo, Faculty of Medicine, As sociate Professor, 医学部(医), 助教授 (00142357)
YOSHIKURA Hiroshi  University of Tokyo, Faculty of Medicine, Professor, 医学部(医), 教授 (60012754)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥12,500,000 (Direct Cost: ¥12,500,000)
Fiscal Year 1994: ¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1993: ¥9,500,000 (Direct Cost: ¥9,500,000)
Keywordshepatitis C virus / HCV / neutralizing antibodies
Research Abstract

We found that HPB-Ma cells are suitable for culturing HCV in vitro. Efficiency of HCV replication are different among subclones of HPB-Ma cells and we established HPB-Ma clone 10 which supports HCV replication very efficiently. We measured the infectivity in vitro of the samples whose Chimpanzee infective titer had been titrated previously and found the correlation between the infectivity of hepatitis C virus in vitro and in vivo. When we innoculated a patient's plasma which contained 6HCV quasispecies, the same two were recovered from the plasma of the infected chimpanzee and culture in vitro. The result confirmed further the correlation between the infectivity in vitro and in vivo.
We established the method to measure the efficiency of adsorption of HCV to the cells and found the correlation between the adsorption eficiency and the infectivity. HCV in the sample with a low adsorption eficiency was present as immune complex with antibodies.
We made a chimeric env gene between HCV and Moloney murine leukemia virus, however, the product stayd in the rough endplasmic reticulum and was not expressed on the cell surface.

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Shimizu, Y. K. , et al.: "Correlation between the infectirty of hepatitis C virus in vivo and its infectivity in vitro." Proc. Natl. Acad. Sci. USA. 90. 6037-6041 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hijikata, M. , et al.: "Equilibrium centrifugation studies of hepatitis C virus:evidence for circulating immune complexes." J. Virol.67. 1953-1958 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimizu, Y. K. , et al.: "Neutralizing antibodies against hepatitis C virus and the emergence of neutralization escape mutant viruses." J. Virol.68. 1494-1500 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimizu, Y. K. , et al.: "Evidence for multicycle infection of hepatitis C virus and inhibition by interferons alpha and beta." J.Virol.68. 8406-8408 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimizu, Y. K. , et al.: "Useful markers for prediciting in vivo infectivity of hepatitis C virus." In“ Viralhcptatitis and liver diseases". 115-117 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimizu, Y.K., Purcell, R.H., and Yoshikura, H.: "Correlation between the infectivity of hepatitis C virus in vivo and its infectivity in vitro." Proc.Natl.Acad.Sci.USA.90. 6037-6041 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Hijikata, M., Shimizu, Y.K., Kato, H., Iwamoto, A., Shih, J.W., Alter.H.J., Purcell, R.H., and Yoshikura, H.: "Equilibrium centrifugation studies of hepatitis C virus : evidence for circulating immune complexes." J.Virol. 67. 1953-1958 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimizu, Y.K., Hijikata, M., Iwamoto, A., Alter, H.J., Purcell, R.H., and Yoshikura, H.: "Neutralizing antibodies agaist hepatitis C virus and the emergence of neutralization escape mutant viruses" J.Virol. 68. 1494-1500 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimizu, Y.K., and Yoshikura, H: "Evidence for multicycle infection of hepatitis C virus and inhibition by interferons alpha and beta." J.Virol. 68. 8406-8408 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimizu, Y.K., Hijikata, M., Iwamoto, A., Alter, H.J., Purcell, R.H., and Yoshikura, H.: "Useful markers for predicting in vivo infectivity of hepatitis C virus." In "Viral hepatitis and liver diseases". 115-117 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimizu,Y.K.,et al.: "Neutralizing antibodiesn against hepatitis C virus and the emergence of neutralization escape mutant viruses." J.Virol.68. 1494-1500 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Shimizu,Y.K.,et al.: "Evidence for multicycle infection of hepatitis C virus and inhibition by interferons alpha and beta." J.Virol.68. 8406-8408 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Matano,T.,et al.: "Interaction between the dominant negative mutant and the wild-type envelope proteins of Friend murine leukemia virus." J.Virol.68. 6079-6082 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] Hijikata,M.et al.: "Equilibrium centrifugation studies of hepatitis C virus:evidence for circulating immune complexes." Journal of Virology. 67. 1953-1958 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shimizu,Y.K.et al.: "Correlation between the infectivity of hepatitis C virus in vivo and its injectivity in vitro" Proc.Natl.Acad.Sci.USA. 90. 6037-6041 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Shimizu,Y.K.et al: "Neutralizing antibodies against hepatitis C virus and the emergence of neutralization escape mutant vineses" Journal of Virology. (in press). (1994)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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