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Preparation of specimens for external quality control of urinary biological monitoring

Research Project

Project/Area Number 05557026
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Public health/Health science
Research InstitutionKawasaki University of Medical Welfare

Principal Investigator

OGATA Masana  Kawasaki University of Medical Welfare Faculty of Medical Welfare Professor, 医療福祉学部, 教授 (70032844)

Co-Investigator(Kenkyū-buntansha) SHIMADA Yoshihiro  Chugoku Junior College Department of Home Economics Assistant professor, 生活学科, 助教授 (80196493)
HUJII Toshiko  Kawasaki University of Medical Welfare Faculty of Medical Profession Assistant p, 医療技術学部, 助教授 (70099638)
TAGUCHI Toyohiro  Kawasaki University of Medical Welfare Faculty of Medical Welfare Instructor, 医療福祉学部, 講師 (30197248)
Project Period (FY) 1993 – 1994
Project Status Completed (Fiscal Year 1994)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1994: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1993: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsBiological monitoring / Quality control / Organic solvent / Solvent metabolites / Artificial prepared Urine / Human urine / Freez dry / Preservation / 溶剤代謝産物 / 空間ガス法 / 人口尿 / 自然尿 / 尿中代謝産物 / 凍結保存 / 常温保存
Research Abstract

Urines from normal human subjects who had not been exposed to organic solvents and artficial urines containing major components of human urine were prepared as specimens for the survey of quality control. Various concentrations of the hippuric acid, methylhippuric acid and mandelic acid were spiked into the normal and artificial urines. Some of specimens were freeze-dried and used for quality control studies.
Thereafter, the specimens were kept at 30゚C,4゚C and -20゚C for 1,2 and 4 weeks, and the changes in concentrations of above described three acids during storage were analyzed by high performance liquid chromatography.
In the case of artificial urines spiked with the three acids, the average concentration of each acid after storage was 97 per cent or more of that before storage under the condition at 30゚C for 1 week, 1゚C for 2 week and -20゚C for 4 weeks. The three acids were stable when they were kept at lower temperature and the mandelic acid was the most stable one among them. The cr … More eatinine was less stable compared to the three acids under the storing condition at 30゚C and 4゚C.
In the case of normal urine spiked with three acids at high and low concentrations, the average concentration of each acid after storage was 99 per cent or more of that before storage under the condition at 30゚C for 1 week and 4゚C and -20゚C for 4 weeks.
When artificial and normal urines spiked with three acids at high and low concentrations were freezedried, the average concentration of each acid after storage at 25゚C was approximately 100 per cent of that before storage in dried artificial urine and was 99 per cent or more of those before storage in dried human urine. The hippuric acid and methylhippuric acid in the urine of workers exposed to low concentrations of mixed toluene and xylenes could be kept without change for 6 months under the freezing condition at -20゚C.
Urinaly metabolites of solvents contained in the specimens prepared for external quality control were proved to be stable after mailing at low temperature. Less

Report

(3 results)
  • 1994 Annual Research Report   Final Research Report Summary
  • 1993 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Ogata, M: "Japanese experience in biological monitoring" Int.Arch.Occup.Environ Health. 65. 15-21 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 田口豊郁: "生物学的モニタリングの為の尿試料の保存-馬尿酸, メチル馬尿酸, 測定に対する冷凍保存の有効性-" 医学と生物学. 128. 131-134 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 嶋田義弘: "有機溶剤の尿中代謝産物を人工尿に添加して作成した精度管理用試料の保存" 川崎医療福祉学会誌. 4. 101-106 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 嶋田義弘: "有機溶剤の尿中代謝産物をヒト尿に添加して作成した精度管理用試料保存" 医学と生物学. 130. 19-21 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 藤井俊子: "有機溶剤の尿中代謝産物を人工尿、人尿に添加後、凍結乾燥して作製した精度管理用試料の保存" 医学と生物学. 130. 23-26 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 嶋田義弘: "外部精度管理用試料を検査所に郵送後の有機溶剤尿中代謝物の濃度変動" 川崎医療福祉学会誌. 5(印刷中). (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] 緒方正名: "最新内科学体系(75)環境因子による疾患" 中山書店, 17 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ogata M: "Japanese experience in biological monitoring" Int.Arch.Occup.Environ.Health. 65. 15-21 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Taguchi T: "Preservation of urine samples for biological monitoring : usefullness of freezing method for huppuric acid methylhippuric acid and creatinine measuring" Medicine and Biology. 128 (3) (in Japanese). 131-134 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada Y: "Preparation and preservation on solvent metabolites spiked in artificcially prepared urines for quality controls" Kawasaki Medical Welfare Journal. 4 (2) (in Japanese). 101-106 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada Y: "Preservation of human urine with solvent metabolites spiked for quality control" Medicine and Biology. 130 (1) (in Japanese). 19-21 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Fujii T: "Preservation of lyophilized human urine with solvent metabolites spiked for quality control" Medicine and Biology. 130 (1) (in Japanese). 23-26 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Shimada Y: "Change in the concentration of urinary metabolites of solvents contained in the specimens prepared for external quality controls after mailing" Kawasaki Medical Welfare Journal. 5 (1) (in press) (in Japanese). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ogata M: "Biological monitoring" Environmental Deasease 1, Integrated Handbook of Internal Medicine, Vol.75 : 33-50 Shunnichi Araki (ed.) Nakayama Shoten, TOKYO. (in Japanese).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1994 Final Research Report Summary
  • [Publications] Ogata,M: "Japanese experience in biological monitoring" Int.Arch.Occup.Environ Health. 65. 15-21 (1993)

    • Related Report
      1994 Annual Research Report
  • [Publications] 田口 豊郁: "生物学的モニタリングの為の尿試料の保存 -馬尿酸,メチル馬尿酸,測定に対する冷凍保存の有効性-" 医学と生物学. 128. 131-134 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 嶋田 義弘: "有機溶剤の尿中代謝産物を人工尿に添加して作製した精度管理用試料の保存" 川崎医療福祉学会誌. 4. 101-106 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 嶋田 義弘: "有機溶剤の尿中代謝産物をヒト尿に添加して作製した精度管理用試料の保存" 医学と生物学. 130. 19-21 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 緒方 正名: "精度管理用試料の保存と利用" 川崎医療福祉学会誌. 5(印刷中). (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 藤井 俊子: "有機溶剤の尿中代謝産物を人工尿、ヒト尿に添加後、凍結乾燥して作製した精度管理用試料の保存" 医学と生物学. 130. 23-26 (1995)

    • Related Report
      1994 Annual Research Report
  • [Publications] 緒方 正名: "最新内科学体系(75) 環境因子による疾患" 中山書店, 17 (1994)

    • Related Report
      1994 Annual Research Report
  • [Publications] 田口 豊郁: "高速液体クロマトグラフによる尿中クレゾール異性体の高感度同時分析" 医学と生物学. 126. 187-191 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] 田口 豊郁: "生物学的モニタリングのための尿試料の保存-馬尿酸,メチル馬尿酸,クレアチニン測定に対する冷凍保存方法の有効性について" 医学と生物学. 128. 131-134 (1994)

    • Related Report
      1993 Annual Research Report
  • [Publications] 緒方 正名: "生物学的モニタリング" 川崎医療福祉学会誌. 3. 11-21 (1993)

    • Related Report
      1993 Annual Research Report
  • [Publications] Ogata,M: "Japanese experience in biological monitoring" Int Arch Occup Environ Health. 65. 15-21 (1993)

    • Related Report
      1993 Annual Research Report

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Published: 1993-04-01   Modified: 2016-04-21  

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